FastQCFastQC Report
Mon 6 Jun 2016
ERR523137_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523137_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524086
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG22200.045.1153532
ACCTGGG26400.037.7387353
GTATCAA19800.032.781261
GTACCTG32350.031.5498451
GTACATG56750.031.411241
GGTATCA13700.030.8982351
TACATGG58400.030.113172
CCTGGGG26700.029.3941334
TGGGGGG23250.029.3089646
ACATGGG61200.027.8747253
TCAACGC22950.027.0300394
CATGGGG47850.026.910644
CAACGCA23450.026.4537035
CTGGGGG16350.026.44395
ATCAACG24650.024.9752463
TATCAAC24950.024.8771762
ATGGGGG30100.024.6686845
GTATATA2101.4188117E-924.6368481
AACGCAG25650.024.3679896
ATATCTA2402.6011548E-1023.5169931
Produced by FastQC (version 0.11.5)