FastQCFastQC Report
Mon 6 Jun 2016
ERR523136_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523136_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8834819
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT387860.4390129554436826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316820.358603837837538No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192910.21835195491837467No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165590.18742885394709274No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG158250.053.460612
GTACCTG178800.047.8002471
ACCTGGG176400.047.6139563
CCTGGGG156800.036.4659164
GGTATCA241750.035.6452521
TATAACG9950.035.4474222
GTATCAA369450.032.083931
ATAACGC10950.031.3516333
TAACGCA12350.028.1750284
TCAACGC437700.026.6102564
ATCAACG440200.026.4195543
CTGGGGG100900.026.3756185
CAACGCA442600.026.3568045
TATCAAC446950.026.1570432
AACGCAG459100.025.5694166
CATGGGG235900.024.8364584
GTACATG389400.024.6420561
TACATGG399050.023.7226352
ATGGGGG146250.023.2444785
TGGGGGG161050.023.2267256
Produced by FastQC (version 0.11.5)