FastQCFastQC Report
Mon 6 Jun 2016
ERR523136_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523136_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8834819
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT352710.3992271941281423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313590.35494784895989384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190940.2161221412685421No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145440.16462136915312017No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG158200.055.2883832
GTACCTG184700.047.93791
ACCTGGG180850.047.4736443
CCTGGGG157750.037.5645564
TATAACG9050.036.3532682
GGTATCA258950.035.2819441
GTATCAA383950.029.4044511
TAACGCA11900.028.8275974
CTGGGGG96750.027.6371865
ATAACGC12650.027.4899353
GTACCCG15900.026.9116211
TCAACGC453700.024.5788484
TGGGGGG149700.024.3284766
ATCAACG460850.024.2077083
CAACGCA462700.024.16175
TATCAAC464100.024.102352
AACGCAG475650.023.6423246
GTACATG394400.023.4630131
CATGGGG235600.022.9579244
TACATGG405900.022.3824062
Produced by FastQC (version 0.11.5)