FastQCFastQC Report
Mon 6 Jun 2016
ERR523135_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523135_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5034320
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT96890.19245896168698057No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA87400.17360835227001858No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG131700.063.4088062
ACCTGGG150200.055.005093
GTACCTG159950.052.367111
CCTGGGG139750.044.4063644
CTGGGGG81000.035.982825
TGGGGGG80650.035.829676
GTACCCG15200.027.5375561
TATAACG3900.026.5246982
ATGGGGG89200.025.3494595
CATGGGG155350.024.5735474
TACCCGG16300.024.5201822
GTACATG286200.021.9541071
TACATGG292750.021.314072
GTATATG13050.021.26281
TACACGG10950.021.0413842
CTGGGGA74750.021.005055
ATAACGC5150.021.0001133
ACATGGG296350.020.4367563
TGGGGGC66400.019.7427776
TATACCG5100.019.356985
Produced by FastQC (version 0.11.5)