##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR523135_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5034320 Sequences flagged as poor quality 0 Sequence length 100 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.361307386101796 33.0 31.0 34.0 28.0 34.0 2 31.692886030288104 34.0 31.0 34.0 27.0 35.0 3 31.692742614692747 34.0 31.0 34.0 27.0 36.0 4 33.99925988018243 35.0 33.0 37.0 31.0 37.0 5 34.07390928665639 35.0 34.0 37.0 31.0 37.0 6 34.294581591952834 35.0 35.0 37.0 30.0 37.0 7 34.16138624481559 35.0 34.0 37.0 30.0 37.0 8 34.24940468623369 35.0 34.0 37.0 30.0 37.0 9 35.43397718063214 37.0 35.0 39.0 30.0 39.0 10-11 35.37865123790303 37.0 34.5 39.0 30.0 39.0 12-13 35.39359953280682 37.0 35.0 39.0 30.0 39.0 14-15 36.18623120898155 38.0 35.0 40.0 30.0 41.0 16-17 36.327886785107026 38.0 36.0 40.0 30.0 41.0 18-19 36.12821801156859 38.0 35.0 40.0 29.5 41.0 20-21 36.19969459629105 38.0 35.0 40.0 29.0 41.0 22-23 36.05504497131688 38.0 35.0 40.0 29.5 41.0 24-25 36.024646526243856 38.0 35.0 40.0 29.0 41.0 26-27 35.91310435172973 37.0 36.0 40.0 28.5 41.0 28-29 35.67304889240255 37.5 34.5 40.0 29.0 41.0 30-31 35.52138104053775 37.0 35.0 40.0 28.0 41.0 32-33 35.52735990163518 37.5 34.5 40.0 27.0 41.0 34-35 35.3974801959351 37.5 34.5 40.0 27.0 41.0 36-37 35.253293990052285 37.5 34.5 40.0 27.0 41.0 38-39 35.11084903224268 36.5 34.5 40.0 27.0 41.0 40-41 35.08191324746937 37.0 33.5 40.0 26.5 41.0 42-43 34.899926405155014 36.0 34.0 40.0 26.0 41.0 44-45 34.84250315832128 37.0 33.5 40.0 26.0 41.0 46-47 34.69921369718254 36.5 33.5 40.0 25.0 41.0 48-49 34.60931863687648 37.0 33.0 39.5 25.5 41.0 50-51 33.550967955950355 36.0 31.5 38.0 24.5 40.0 52-53 33.775221877036024 36.0 32.5 38.5 25.0 39.5 54-55 34.68004050199431 37.0 33.0 39.0 26.0 40.5 56-57 34.814775183142906 36.5 34.0 40.0 26.0 41.0 58-59 34.712184167871726 37.0 33.0 39.0 26.0 41.0 60-61 34.418586323475665 36.5 34.0 39.0 26.0 41.0 62-63 34.18743713152918 36.0 33.0 39.0 26.0 41.0 64-65 33.772193066789555 35.5 33.0 38.0 25.5 40.0 66-67 33.55224280538384 35.5 33.0 37.0 25.5 40.0 68-69 33.1943938804049 35.0 32.5 37.0 24.5 39.0 70-71 32.96532908118674 35.0 32.0 37.0 24.5 39.0 72-73 32.636653510305266 35.0 32.0 36.5 24.0 38.5 74-75 32.27008434108281 35.0 32.0 36.0 24.0 37.5 76-77 32.16049516121343 35.0 31.0 36.5 22.5 38.0 78-79 31.907658432519188 35.0 31.0 36.0 23.0 37.0 80-81 31.668522958413448 34.5 31.0 35.5 21.0 37.5 82-83 31.390414892180075 34.0 30.5 35.0 20.5 37.0 84-85 31.185284805097808 34.0 30.0 35.0 20.0 36.5 86-87 30.962573793481543 34.0 30.0 35.0 20.0 37.0 88-89 30.828036557072256 34.0 30.0 35.0 19.0 36.5 90-91 30.622086398957556 34.0 29.5 35.0 18.5 36.0 92-93 30.364317623830033 34.0 29.0 35.0 18.0 35.5 94-95 30.27693620985555 34.0 29.0 35.0 18.0 36.0 96-97 30.080591917081154 34.0 29.0 35.0 17.0 35.0 98-99 29.989576447265968 34.0 29.0 35.0 17.5 36.0 100 28.594573050580813 33.0 25.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08065705062328021 1101 2 0.08509243274139067 1101 3 0.17581242319320012 1101 4 0.16811620615226985 1101 5 0.15254552556989154 1101 6 0.17480454047072413 1101 7 0.17521206988814697 1101 8 0.22115473624960913 1101 9 0.27602628770948456 1101 10-11 0.17780317554611003 1101 12-13 0.08570399657097738 1101 14-15 0.02350886320792256 1101 16-17 0.10849072865841691 1101 18-19 0.13557286346910047 1101 20-21 0.01232604134295201 1101 22-23 0.07972875152835002 1101 24-25 -0.03548645396282524 1101 26-27 -0.10705406355942415 1101 28-29 -0.15210390133929508 1101 30-31 -0.16688361752231629 1101 32-33 -0.1406385306213238 1101 34-35 -0.21542967213818542 1101 36-37 -0.2729620464822844 1101 38-39 -0.2330484411724001 1101 40-41 -0.1892092105521499 1101 42-43 -0.1766194271644892 1101 44-45 -0.27182462037524147 1101 46-47 -0.29447177333030794 1101 48-49 -0.310159720728052 1101 50-51 -0.41810298938932533 1101 52-53 -0.2935344087973206 1101 54-55 -0.18786222229046956 1101 56-57 -0.3070913565744391 1101 58-59 -0.23947454475676722 1101 60-61 -0.254248753216892 1101 62-63 -0.33472250874774545 1101 64-65 -0.3443646726740397 1101 66-67 -0.30525585467557903 1101 68-69 -0.3087347594035066 1101 70-71 -0.34756715674020455 1101 72-73 -0.328844309746934 1101 74-75 -0.4538494144294347 1101 76-77 -0.2616854416056107 1101 78-79 -0.3783700541652735 1101 80-81 -0.415666790334992 1101 82-83 -0.6730974006190813 1101 84-85 -0.534859244906297 1101 86-87 -0.4770811736546321 1101 88-89 -0.7959528378585823 1101 90-91 -0.7340707628482228 1101 92-93 -0.6383276573487748 1101 94-95 -0.6611239545882235 1101 96-97 -0.7328814131937982 1101 98-99 -0.7322203152323254 1101 100 -0.7044363654104338 1102 1 -0.04080820007620645 1102 2 -0.004984286040361496 1102 3 -0.023487784109704535 1102 4 -0.019450821541056484 1102 5 -0.03642417496273964 1102 6 0.031802372280665736 1102 7 0.06447987804953215 1102 8 0.10513463909659748 1102 9 -0.06453510708825405 1102 10-11 0.011869304814062787 1102 12-13 -0.028476533111948754 1102 14-15 -0.15933143404114247 1102 16-17 -0.02491933966927462 1102 18-19 -0.05581185355069351 1102 20-21 -0.1334606216977292 1102 22-23 -0.0935171408479647 1102 24-25 -0.23284497549109062 1102 26-27 -0.03232423086723202 1102 28-29 -0.06746482896006256 1102 30-31 -0.1990067076150268 1102 32-33 -0.12553231722261415 1102 34-35 -0.17617354763040538 1102 36-37 -0.6557176716063893 1102 38-39 -0.2502761945971912 1102 40-41 -0.8757182384742848 1102 42-43 -0.9681010180615175 1102 44-45 -0.39954418900418176 1102 46-47 -0.4692005698251691 1102 48-49 -0.6146293896460904 1102 50-51 -0.6032217999870397 1102 52-53 -0.36051348543553985 1102 54-55 -0.24269869449108938 1102 56-57 -0.03336371702561536 1102 58-59 -0.14606672645756902 1102 60-61 -0.28276860453737385 1102 62-63 -0.5260645936486839 1102 64-65 -0.22876461608498033 1102 66-67 -0.27179207326161503 1102 68-69 -0.317005601931335 1102 70-71 -0.42847247177696346 1102 72-73 -0.3465188968381554 1102 74-75 -0.263311482099887 1102 76-77 -0.43681769950709537 1102 78-79 -0.40496756911030474 1102 80-81 -0.48665233453631274 1102 82-83 -0.5657166516006633 1102 84-85 -0.6665561618385851 1102 86-87 -0.712294415664438 1102 88-89 -0.64757280550808 1102 90-91 -0.6826537418478829 1102 92-93 -0.5697560511435142 1102 94-95 -0.8193037492054565 1102 96-97 -0.9359512218081498 1102 98-99 -0.8775757510072282 1102 100 -0.823256267758353 1103 1 0.04224483226700215 1103 2 0.07395559464080037 1103 3 0.11543387073186295 1103 4 0.07036478860976558 1103 5 -0.058269060474771095 1103 6 0.07531158666785132 1103 7 0.07333608749183185 1103 8 0.03747044380953213 1103 9 0.11353594050186189 1103 10-11 0.02791472306176246 1103 12-13 0.16853577358417482 1103 14-15 0.11555509053714985 1103 16-17 0.008372024031089609 1103 18-19 0.10972956747769302 1103 20-21 -0.055307464232690506 1103 22-23 7.608219252048798E-4 1103 24-25 -0.11859357292254202 1103 26-27 -0.06200858859570957 1103 28-29 -0.28092889142482846 1103 30-31 -0.26806362252153804 1103 32-33 -0.3150419936373865 1103 34-35 -0.39258986426650466 1103 36-37 -0.4132312995057106 1103 38-39 -0.45855529499630165 1103 40-41 -0.23452911730946369 1103 42-43 -0.32609769027165925 1103 44-45 -0.24670888822464576 1103 46-47 -0.27940876302928785 1103 48-49 -0.3706797812846574 1103 50-51 -0.392605737678835 1103 52-53 -0.29769353877717464 1103 54-55 -0.21214220651338422 1103 56-57 -0.04615464869191044 1103 58-59 -0.11776428217847723 1103 60-61 -0.031352528274744884 1103 62-63 -0.132678735521786 1103 64-65 -0.2077648666690024 1103 66-67 -0.29831420102203055 1103 68-69 -0.3496470851164659 1103 70-71 -0.17177949591514619 1103 72-73 -0.13174324611740218 1103 74-75 -0.14173528307235728 1103 76-77 -0.21304010495204295 1103 78-79 -0.2953157447607708 1103 80-81 -0.3041687745924868 1103 82-83 -0.1985644518005394 1103 84-85 -0.2308688490054891 1103 86-87 -0.9319609006683294 1103 88-89 -0.5688966762101266 1103 90-91 -0.8234021074146618 1103 92-93 -0.4056971813412744 1103 94-95 -0.7152220588141383 1103 96-97 -0.5376817831461409 1103 98-99 -0.5681537279475428 1103 100 -0.5688937020332254 1104 1 0.016345308616006804 1104 2 -0.015498971441356701 1104 3 0.010310046947420659 1104 4 0.051882139551622686 1104 5 -0.05472674912942921 1104 6 0.08433547556307985 1104 7 0.04894666444750584 1104 8 0.04900135439969944 1104 9 -0.031253368958282124 1104 10-11 -0.19696962135760288 1104 12-13 -0.09871044029046772 1104 14-15 -0.17300324712505244 1104 16-17 -0.14115741532317827 1104 18-19 -0.020336509140030046 1104 20-21 -0.16068097308896512 1104 22-23 -0.06546852582412299 1104 24-25 -0.18839677858282755 1104 26-27 -0.0975104237541089 1104 28-29 -0.12292913718209064 1104 30-31 -0.11095254702262736 1104 32-33 -0.142289444567389 1104 34-35 -0.20905016760637096 1104 36-37 -0.20039170672183815 1104 38-39 -0.16122936878003458 1104 40-41 -0.09175750634088331 1104 42-43 -0.329466474833346 1104 44-45 -0.2400385054304337 1104 46-47 -0.1379401533918596 1104 48-49 -0.16623223648399232 1104 50-51 -0.22695195143769809 1104 52-53 -0.30790573677152366 1104 54-55 -0.1417426756682829 1104 56-57 -0.16684238201452928 1104 58-59 -0.20490003097817322 1104 60-61 -0.16273673865222094 1104 62-63 -0.10286723100611539 1104 64-65 -0.20994087682476703 1104 66-67 -0.1279609687931611 1104 68-69 -0.16374743246771573 1104 70-71 -0.04534425121550356 1104 72-73 -0.0405181858895034 1104 74-75 -0.08699277168127395 1104 76-77 -0.20568330527380851 1104 78-79 -0.19354342314688466 1104 80-81 -0.15750519394896756 1104 82-83 -0.2021077967817071 1104 84-85 -0.2002282031699778 1104 86-87 -0.25077698410804317 1104 88-89 -0.15480057916941803 1104 90-91 -0.35874608570864197 1104 92-93 -0.3723482028959708 1104 94-95 -0.4491949977318832 1104 96-97 -0.6524235470117894 1104 98-99 -0.43768250742869697 1104 100 -0.287458396969253 1105 1 0.12640457389992577 1105 2 0.09504719089992619 1105 3 0.06958986633316755 1105 4 0.14242101280827058 1105 5 0.11139162113767043 1105 6 0.20209574581434708 1105 7 0.017664288133644845 1105 8 -0.029724783620544315 1105 9 0.07879579540843906 1105 10-11 0.15405622122313645 1105 12-13 0.2231969627128123 1105 14-15 0.22215461132282854 1105 16-17 0.16701857090409788 1105 18-19 0.12468116828627274 1105 20-21 0.16345495503586704 1105 22-23 -0.040950397059269505 1105 24-25 0.03310500279192752 1105 26-27 0.03566889659705197 1105 28-29 0.01005448816356136 1105 30-31 -0.07090039836866424 1105 32-33 -0.13241514103794572 1105 34-35 -0.031347934104694275 1105 36-37 -0.1008381754740384 1105 38-39 -0.08746655868188213 1105 40-41 0.010657992506466485 1105 42-43 -0.01547252454364667 1105 44-45 -0.18253751504781945 1105 46-47 -0.043753576147601336 1105 48-49 -0.06257793815775159 1105 50-51 -0.1653885035528475 1105 52-53 -0.08645314604229526 1105 54-55 0.11999631466045457 1105 56-57 0.15015757659634943 1105 58-59 0.11124488088598383 1105 60-61 0.07976673836269299 1105 62-63 0.06961093951576203 1105 64-65 0.11641565171829171 1105 66-67 0.2211396636063725 1105 68-69 0.20273453796993834 1105 70-71 0.1310483942656262 1105 72-73 0.016577453864805136 1105 74-75 0.02486839868490165 1105 76-77 0.015264362777529072 1105 78-79 0.07653087690061255 1105 80-81 0.08894872714497026 1105 82-83 0.03464932592521919 1105 84-85 -0.03652403423402717 1105 86-87 0.018085246936561106 1105 88-89 -0.1455458806826151 1105 90-91 -0.08281740081192623 1105 92-93 -0.1561752301911099 1105 94-95 0.009141264593697684 1105 96-97 -0.0902966239778209 1105 98-99 -0.06756285179935162 1105 100 -0.0663457062097379 1106 1 0.18573790485474007 1106 2 0.10910769365214534 1106 3 0.13899994836293672 1106 4 0.12869746263277904 1106 5 -0.02255771118457517 1106 6 0.19496942788421023 1106 7 0.11680558004007224 1106 8 -1.0347798831308168 1106 9 0.1728459451092803 1106 10-11 0.09018309183496598 1106 12-13 0.09444558303572848 1106 14-15 0.08584695557865274 1106 16-17 0.11323222697456714 1106 18-19 0.16796218146468078 1106 20-21 0.15569898459785492 1106 22-23 0.1679068459737394 1106 24-25 0.05044312041128762 1106 26-27 0.007002973380238586 1106 28-29 -0.023954551782289002 1106 30-31 0.11502669678632316 1106 32-33 0.14369138592523 1106 34-35 0.10215737167195726 1106 36-37 0.1038370810575131 1106 38-39 0.1162244565755799 1106 40-41 0.15133263229844118 1106 42-43 0.10500127918753321 1106 44-45 0.004004718233801441 1106 46-47 -0.023970616420392332 1106 48-49 0.02895185816412038 1106 50-51 0.09911991786952967 1106 52-53 0.18105456279695176 1106 54-55 0.1990068084770087 1106 56-57 0.2051651354266184 1106 58-59 0.2654272606541994 1106 60-61 0.230831526344069 1106 62-63 0.23290340445988278 1106 64-65 0.16973062287278395 1106 66-67 0.2058518645087659 1106 68-69 0.21219467110005752 1106 70-71 0.158474858247736 1106 72-73 0.17990575878942394 1106 74-75 0.18824681471345173 1106 76-77 0.16705578203751514 1106 78-79 0.21689933622580782 1106 80-81 0.1870798505515907 1106 82-83 0.28817526644902003 1106 84-85 0.1749727179501832 1106 86-87 0.21360667243367715 1106 88-89 0.15987973692457658 1106 90-91 0.17092084294469956 1106 92-93 0.2283999436003583 1106 94-95 0.17417010361510066 1106 96-97 0.07971423586061022 1106 98-99 0.05665099985037614 1106 100 0.2185423254747505 1107 1 0.08962209445671832 1107 2 -0.04320481088235084 1107 3 0.010161050392223814 1107 4 0.21417578732248188 1107 5 0.11191195818365429 1107 6 0.05060785534566037 1107 7 0.1858615389756082 1107 8 0.1643788068730032 1107 9 0.1603744006076795 1107 10-11 0.14270408538629908 1107 12-13 0.12586496752610543 1107 14-15 0.06045759526027439 1107 16-17 0.15431691331034614 1107 18-19 0.14626749897529834 1107 20-21 0.22686068694785888 1107 22-23 0.10059199485664294 1107 24-25 0.2244965953223641 1107 26-27 0.2198001800247269 1107 28-29 0.09501582393041019 1107 30-31 0.22783322025043162 1107 32-33 0.2027728975912666 1107 34-35 0.1584141966175281 1107 36-37 0.25828192874370615 1107 38-39 0.1669134845571989 1107 40-41 0.16530249043199063 1107 42-43 0.179341541525325 1107 44-45 0.16313005331248576 1107 46-47 0.21328914618673167 1107 48-49 0.10516138889538951 1107 50-51 -0.006588346244257082 1107 52-53 0.11551723368939548 1107 54-55 0.19716656874998506 1107 56-57 0.1331052031972959 1107 58-59 0.32587171757899824 1107 60-61 0.23938706424545586 1107 62-63 0.2827997523732293 1107 64-65 0.27586381137084715 1107 66-67 0.23086831507379202 1107 68-69 0.23737905348792765 1107 70-71 0.0974577072032119 1107 72-73 0.2073210040684188 1107 74-75 0.17233385268717427 1107 76-77 0.2899741551257051 1107 78-79 0.29343477791711337 1107 80-81 0.2560079056281701 1107 82-83 0.14456774807969808 1107 84-85 0.19407044064537615 1107 86-87 0.23504582366864213 1107 88-89 0.14415328901715085 1107 90-91 0.15757229701035058 1107 92-93 0.2450745822892344 1107 94-95 0.1752628848018709 1107 96-97 0.2239411114373091 1107 98-99 0.3197787600667965 1107 100 0.3051830211611595 1108 1 -0.07619985185869638 1108 2 -0.14024358047867125 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-0.17590629226931043 2316 66-67 -0.12578133738249875 2316 68-69 0.08299364887961502 2316 70-71 0.16904455798022866 2316 72-73 0.032231637205484276 2316 74-75 0.06753470130435346 2316 76-77 -0.06485519287036112 2316 78-79 -0.12904214812027703 2316 80-81 -0.02564880763027233 2316 82-83 0.0890409058931887 2316 84-85 0.20509805350319255 2316 86-87 0.34994245386093326 2316 88-89 0.28902841160867965 2316 90-91 0.39979200947142957 2316 92-93 0.594524231521568 2316 94-95 0.6720439650088927 2316 96-97 0.6214717728937025 2316 98-99 0.6710592363975216 2316 100 0.3120259823166158 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 8885.0 1 805.0 2 513.0 3 424.0 4 327.0 5 317.0 6 360.0 7 594.0 8 1342.0 9 2047.0 10 3447.0 11 5905.0 12 8702.0 13 11505.0 14 13794.0 15 15949.0 16 18332.0 17 21042.0 18 24449.0 19 27853.0 20 31539.0 21 36047.0 22 40893.0 23 47462.0 24 54453.0 25 63821.0 26 74843.0 27 88666.0 28 105677.0 29 126871.0 30 155943.0 31 192461.0 32 247211.0 33 330466.0 34 475793.0 35 732272.0 36 748165.0 37 759976.0 38 506768.0 39 48397.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.341379962004794 15.038104616119119 10.98459304349556 25.635922378380528 2 17.521138418400845 18.32256658972018 37.9132494455619 26.243045546317074 3 20.994502768540453 23.530501764334343 26.835953766669522 28.63904170045568 4 14.147294042356407 13.86056253251407 33.225987506228854 38.766155918900665 5 15.185457487039095 35.0642765205402 31.70456311492068 18.04570287750003 6 35.01383462731342 30.979508341999473 18.35713850439454 15.649518526292564 7 32.507116368304274 28.93272199145996 19.30830931339144 19.251852326844332 8 29.925002489296027 31.740017922931397 18.769172558000598 19.56580702977198 9 29.654687415044094 14.643993302512037 17.622849850039444 38.078469432404425 10-11 28.14046627552348 23.28654661834308 26.13254790087008 22.440439205263356 12-13 27.88760955381123 20.811993104215993 25.91915077587527 25.38124656609751 14-15 25.330982247804467 22.30529683085244 23.524934062363627 28.838786858979464 16-17 25.830257678087854 24.590737255318906 24.17052878174187 25.40847628485137 18-19 25.67355654222887 24.72845962205283 24.667157060607394 24.9308267751109 20-21 26.44241209458475 24.118894756844693 24.20233232906225 25.236360819508302 22-23 26.124568369718837 23.869269039022043 23.955127176370375 26.05103541488874 24-25 25.954814140545064 23.964468734969767 23.909253542568315 26.171463581916854 26-27 25.783748034612913 23.723547480257523 24.41827877439691 26.07442571073265 28-29 25.63446444519894 24.515134649141583 24.133773949545528 25.71662695611395 30-31 26.55215393314803 24.008748735674292 24.096972786135822 25.342124545041848 32-33 26.164390217481348 24.098062548985872 24.171362141805737 25.566185091727046 34-35 26.240811394597408 24.220587453543054 24.190573482860977 25.34802766899856 36-37 26.38293123260117 24.048827527269157 24.213026873020432 25.355214367109234 38-39 26.22969523551431 24.38550954718057 23.941818603220767 25.442976614084355 40-41 26.045538156625025 24.12558615965688 24.114045437386903 25.71483024633119 42-43 25.847054402998314 24.098913564877567 23.957416745170917 26.096615286953202 44-45 25.70685980348854 23.982369854186754 24.268606874645922 26.04216346767878 46-47 25.753001148703607 23.782903280430318 24.40659190022097 26.057503670645104 48-49 25.802839555939943 23.902222520456096 24.487986589829852 25.806951333774116 50-51 25.263421759633363 24.115797560441184 24.90985327744225 25.710927402483204 52-53 25.487469452259496 24.186273111934568 24.23868618429647 26.087571251509466 54-55 25.205757567247993 23.90103800398281 24.648367412661703 26.244837016107493 56-57 25.592908567088795 23.868493713762685 24.353295887052955 26.185301832095565 58-59 25.70297943795101 23.91328937819975 24.443580821924353 25.94015036192488 60-61 25.96492985084151 23.94416380046776 24.726128633951795 25.364777714738935 62-63 25.491061843938073 24.112696965460266 24.613059638028478 25.783181552573183 64-65 25.31675782182341 24.057403701589415 24.535180394008943 26.090658082578237 66-67 25.54508907001764 23.854216580968416 24.435034830971503 26.16565951804244 68-69 25.179986862970587 23.862410131047646 24.58231774960854 26.375285256373232 70-71 25.16967821573848 23.605215235176928 24.924890975751463 26.30021557333313 72-73 25.103789020184035 23.871469661690178 24.7644282850419 26.260313033083886 74-75 24.994890916587494 24.070654739496504 24.730951484621958 26.203502859294048 76-77 24.894631838100167 23.511094915648208 24.944536127628304 26.649737118623325 78-79 24.776251542363077 23.68957148448276 25.114590480818926 26.419586492335238 80-81 25.00749050132738 23.787054083660014 24.798114932818493 26.407340482194115 82-83 24.85531242120308 23.803740745033977 24.960613987601224 26.380332846161718 84-85 24.827128414481475 24.03396579318752 24.59896680407422 26.539938988256782 86-87 24.728716779052572 23.922497991573522 24.850621569031464 26.498163660342446 88-89 24.388452630357584 23.68321273077036 25.0285666479592 26.899767990912853 90-91 24.957522274601153 23.484791824039988 25.04408610770983 26.51359979364903 92-93 24.82324584815557 23.573174531152315 25.141342085933477 26.462237534758636 94-95 24.605068744231147 23.3825794832142 25.218703577775415 26.79364819477924 96-97 24.375275801446264 23.3214864651016 25.467984823712648 26.835252909739495 98-99 24.556125461658795 23.35952179666799 25.09798767051129 26.98636507116192 100 24.526763152993343 23.147575076368685 25.188568651140276 27.137093119497695 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2406.0 1 3359.5 2 3172.5 3 1207.0 4 335.5 5 265.0 6 226.0 7 204.5 8 187.5 9 178.5 10 168.0 11 157.0 12 164.0 13 167.5 14 161.5 15 174.0 16 182.5 17 180.5 18 189.5 19 179.5 20 203.5 21 242.0 22 254.0 23 276.0 24 297.0 25 341.5 26 402.0 27 493.5 28 646.0 29 901.5 30 1333.5 31 2147.0 32 3620.0 33 5913.5 34 9428.5 35 14797.5 36 23006.0 37 32667.5 38 43576.0 39 56363.0 40 69329.0 41 83114.5 42 100149.0 43 119564.0 44 138439.0 45 157411.5 46 179703.0 47 207379.0 48 243085.5 49 268472.0 50 285508.5 51 289898.5 52 277962.0 53 274981.0 54 274025.5 55 262683.0 56 243373.5 57 222932.0 58 204482.0 59 183078.0 60 155691.0 61 129081.0 62 106158.5 63 84292.0 64 63987.5 65 48171.5 66 35945.0 67 27798.5 68 21596.0 69 16191.0 70 12469.0 71 9688.0 72 7695.0 73 6037.0 74 4663.0 75 3471.5 76 2413.5 77 1508.0 78 901.0 79 610.5 80 397.5 81 265.0 82 186.5 83 120.0 84 86.5 85 70.0 86 58.5 87 50.0 88 35.5 89 27.5 90 28.5 91 22.5 92 14.0 93 11.5 94 12.0 95 9.5 96 5.5 97 5.0 98 2.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14639514373341386 2 0.07053584198064485 3 0.30649621001446076 4 0.30468861733064245 5 0.305503027221154 6 0.25548634175022644 7 0.2542150677747938 8 0.2546520682038488 9 0.26484212366317594 10-11 0.24240612436237666 12-13 0.2522882931557787 14-15 0.2262867676270082 16-17 0.234649366746651 18-19 0.24843474391774856 20-21 0.2503118593970983 22-23 0.23999269017464125 24-25 0.24137321425733763 26-27 0.2401615312495034 28-29 0.2495868359579844 30-31 0.23598023168968202 32-33 0.24758060671550478 34-35 0.2315903637432662 36-37 0.23977418996011376 38-39 0.24095607748414882 40-41 0.25764154841170206 42-43 0.24430310349759254 44-45 0.22961392998458582 46-47 0.23243456911757696 48-49 0.24157185081599897 50-51 0.23465929857458406 52-53 0.2517122471356608 54-55 0.26301466732349155 56-57 0.2580984124966232 58-59 0.2549698866977069 60-61 0.23525520825056812 62-63 0.2634318040966804 64-65 0.25696618411225347 66-67 0.2624783486151059 68-69 0.26613326129447473 70-71 0.28703975909358165 72-73 0.29562085842775193 74-75 0.27511163374596764 76-77 0.27232079009677573 78-79 0.27044367461742597 80-81 0.25744291185304075 82-83 0.26819908150455274 84-85 0.27759459072923454 86-87 0.27011592429563475 88-89 0.27893538750019864 90-91 0.27307560901968886 92-93 0.25279481638036516 94-95 0.2746349060051805 96-97 0.2726187449347678 98-99 0.2587241176564064 100 0.28335505093041363 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5034320.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.908195382970774 #Duplication Level Percentage of deduplicated Percentage of total 1 68.1624209940606 34.018634247478865 2 17.45145975869824 17.419417267103274 3 6.383166733677024 9.55716997519297 4 2.843664334985398 5.676886209361476 5 1.3958902044643184 3.483318052879012 6 0.852148752575101 2.5517523863323777 7 0.5459541631912225 1.9073310932685692 8 0.3820894285962587 1.5255515084919795 9 0.2901291722398075 1.3031841072999504 >10 1.5503189570399176 14.063999567809663 >50 0.09706733440782564 3.320800524549873 >100 0.04391163392991747 3.7216160723242173 >500 0.0010778982748305924 0.3644138153230099 >1k 5.928440349904743E-4 0.5902223338459343 >5k 1.0778982454372259E-4 0.4957028387388561 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9689 0.19245896168698057 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 8740 0.17360835227001858 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.986365586613485E-5 0.0 5 0.0 1.986365586613485E-5 0.0 1.986365586613485E-5 0.0 6 0.0 1.986365586613485E-5 0.0 1.986365586613485E-5 5.9590967598404554E-5 7 0.0 1.986365586613485E-5 0.0 1.986365586613485E-5 5.9590967598404554E-5 8 0.0 1.986365586613485E-5 0.0 1.986365586613485E-5 5.9590967598404554E-5 9 0.0 1.986365586613485E-5 0.0 1.986365586613485E-5 7.94546234645394E-5 10-11 0.0 1.986365586613485E-5 0.0 1.986365586613485E-5 7.94546234645394E-5 12-13 0.0 1.986365586613485E-5 0.0 5.9590967598404554E-5 8.938645139760682E-5 14-15 0.0 1.986365586613485E-5 0.0 6.952279553147197E-5 9.931827933067425E-5 16-17 0.0 1.986365586613485E-5 0.0 7.94546234645394E-5 1.589092469290788E-4 18-19 0.0 1.986365586613485E-5 0.0 7.94546234645394E-5 1.7877290279521364E-4 20-21 0.0 1.986365586613485E-5 0.0 7.94546234645394E-5 1.7877290279521364E-4 22-23 0.0 1.986365586613485E-5 0.0 8.938645139760682E-5 2.1850021452748334E-4 24-25 0.0 1.986365586613485E-5 0.0 1.7877290279521364E-4 2.9795483799202274E-4 26-27 0.0 1.986365586613485E-5 0.0 3.97273117322697E-4 2.9795483799202274E-4 28-29 0.0 4.965913966533712E-5 0.0 0.0013904559106294396 2.9795483799202274E-4 30-31 0.0 7.94546234645394E-5 0.0 0.004568640849211015 3.078866659250902E-4 32-33 0.0 7.94546234645394E-5 0.0 0.013626467924168507 4.568640849211016E-4 34-35 0.0 7.94546234645394E-5 0.0 0.0318910994930795 4.965913966533713E-4 36-37 0.0 7.94546234645394E-5 0.0 0.065401086939249 5.065232245864387E-4 38-39 0.0 8.938645139760682E-5 0.0 0.1381815220327671 5.36318708385641E-4 40-41 0.0 1.191819351968091E-4 0.0 0.24567369575235581 5.36318708385641E-4 42-43 0.0 1.3904559106294394E-4 0.0 0.35322546043954295 5.462505363187084E-4 44-45 0.0 1.3904559106294394E-4 0.0 0.48936102591809816 5.561823642517758E-4 46-47 9.931827933067425E-6 1.3904559106294394E-4 0.0 0.6572287816428037 5.661141921848433E-4 48-49 1.986365586613485E-5 1.3904559106294394E-4 0.0 0.8362797756201434 5.959096759840455E-4 50-51 1.986365586613485E-5 1.3904559106294394E-4 0.0 1.0369821544915698 6.058415039171129E-4 52-53 1.986365586613485E-5 1.3904559106294394E-4 0.0 1.251767865372086 6.455688156493827E-4 54-55 1.986365586613485E-5 1.589092469290788E-4 0.0 1.5003118593970983 6.852961273816523E-4 56-57 1.986365586613485E-5 1.589092469290788E-4 0.0 1.7951083761064055 6.952279553147198E-4 58-59 1.986365586613485E-5 1.589092469290788E-4 0.0 2.1241696991847956 6.952279553147198E-4 60-61 1.986365586613485E-5 1.589092469290788E-4 0.0 2.461822053425289 7.150916111808546E-4 62-63 1.986365586613485E-5 1.589092469290788E-4 0.0 2.8118991244100493 7.548189229131243E-4 64-65 1.986365586613485E-5 1.589092469290788E-4 0.0 3.1679352921546506 7.548189229131243E-4 66-67 1.986365586613485E-5 1.7877290279521364E-4 0.0 3.5867803397479703 7.647507508461917E-4 68-69 1.986365586613485E-5 1.7877290279521364E-4 0.0 4.075396478571088 7.746825787792592E-4 70-71 1.986365586613485E-5 1.7877290279521364E-4 0.0 4.619183921562396 7.746825787792592E-4 72-73 1.986365586613485E-5 1.7877290279521364E-4 0.0 5.113630043382225 8.938645139760683E-4 74-75 1.986365586613485E-5 1.7877290279521364E-4 0.0 5.611492714011028 8.938645139760683E-4 76-77 1.986365586613485E-5 1.7877290279521364E-4 0.0 6.157792909469402 9.037963419091357E-4 78-79 1.986365586613485E-5 2.1850021452748334E-4 0.0 6.776525926043636 9.33591825708338E-4 80-81 1.986365586613485E-5 2.1850021452748334E-4 0.0 7.412719096124204 9.534554815744728E-4 82-83 1.986365586613485E-5 2.1850021452748334E-4 0.0 8.066779624656359 0.0010031146212398099 84-85 1.986365586613485E-5 2.1850021452748334E-4 0.0 8.741240127763035 0.0010229782771059449 86-87 2.9795483799202274E-5 2.1850021452748334E-4 0.0 9.426387277725691 0.0010825692447043495 88 3.97273117322697E-5 2.1850021452748334E-4 0.0 9.978090387579654 0.0011520920402358214 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 13170 0.0 63.408806 2 ACCTGGG 15020 0.0 55.00509 3 GTACCTG 15995 0.0 52.36711 1 CCTGGGG 13975 0.0 44.406364 4 CTGGGGG 8100 0.0 35.98282 5 TGGGGGG 8065 0.0 35.82967 6 GTACCCG 1520 0.0 27.537556 1 TATAACG 390 0.0 26.524698 2 ATGGGGG 8920 0.0 25.349459 5 CATGGGG 15535 0.0 24.573547 4 TACCCGG 1630 0.0 24.520182 2 GTACATG 28620 0.0 21.954107 1 TACATGG 29275 0.0 21.31407 2 GTATATG 1305 0.0 21.2628 1 TACACGG 1095 0.0 21.041384 2 CTGGGGA 7475 0.0 21.00505 5 ATAACGC 515 0.0 21.000113 3 ACATGGG 29635 0.0 20.436756 3 TGGGGGC 6640 0.0 19.742777 6 TATACCG 510 0.0 19.35698 5 >>END_MODULE