FastQCFastQC Report
Mon 6 Jun 2016
ERR523135_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523135_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5034320
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT98690.19603441974288485No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA84910.168662301959351No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG137150.064.184342
ACCTGGG155650.056.036183
GTACCTG163650.054.3890651
CCTGGGG141150.046.0781334
CTGGGGG76850.037.7294925
TGGGGGG80000.034.187926
GTACCCG15850.029.0719261
TATAACG3500.028.1993052
TACCCGG16650.027.0984292
CATGGGG156350.023.925164
ATGGGGG90650.023.6912565
GTACATG285000.022.2558421
TACCGTA5500.022.2152047
CTGGGGA75650.021.8040835
TACATGG291650.021.5777282
GTATAAC8500.021.5736031
TACCGGG8850.021.2424142
TGGGGGC60950.021.1259636
GTACCGG12850.020.856851
TGGGGAG64500.020.5460856
Produced by FastQC (version 0.11.5)