FastQCFastQC Report
Mon 6 Jun 2016
ERR523134_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523134_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7047158
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193080.2739827885226924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147920.20990021793182442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88640.12578120144319171No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105800.049.505531
GTATCAA184550.040.569571
TCAACGC219900.033.533064
CAACGCA224700.032.81585
ATCAACG224750.032.749263
TATCAAC231100.031.9768852
AACGCAG234000.031.8046936
TACCTGG67650.031.5479872
GTACATG237300.028.477441
ACGCAGA271750.027.2309577
TACATGG245400.026.7995032
GTACCTG83750.025.9036121
CGCAGAG285650.025.8881368
ACATGGG248500.025.7797623
CATGGGG184800.024.0582854
ACCTGGG89450.023.2247683
GCAGAGT316950.023.0341429
ATGGGGG114150.022.1909525
TATAACG5150.020.9944342
GAGTACT179600.020.92733412-13
Produced by FastQC (version 0.11.5)