FastQCFastQC Report
Mon 6 Jun 2016
ERR523134_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523134_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7047158
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174930.24822772527591974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143540.2036849464706198No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83360.11828881940776693No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103550.049.5282671
GTATCAA179550.039.596411
TCAACGC216650.032.2101364
CAACGCA221100.031.6681235
ATCAACG220800.031.6266943
TACCTGG65650.031.0206552
TATCAAC228900.030.7180182
AACGCAG230650.030.519476
GTACATG232650.027.8690951
TACATGG233300.027.0542552
ACGCAGA266400.026.3883917
ACATGGG239150.025.7612153
CGCAGAG281000.024.9571578
GTACCTG83250.024.4726141
CATGGGG176650.023.728814
ACCTGGG88200.022.4842223
ATGGGGG109200.022.118995
GCAGAGT313450.022.0883889
GAGTACT182100.020.81801812-13
AGAGTAC282400.019.48267610-11
Produced by FastQC (version 0.11.5)