FastQCFastQC Report
Mon 6 Jun 2016
ERR523133_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523133_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4643976
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202350.43572576602463065No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170120.3663240292370159No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92980.20021636632058393No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63740.13725307796594988No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91000.049.6974141
TACCTGG47750.044.4977872
GTATCAA163700.041.022971
ACCTGGG56450.036.717493
TATAACG6500.034.7137262
TATCACG4850.033.9234242
TCAACGC198600.033.4378134
GTACCTG65500.032.9060631
ATCAACG203600.032.595323
CAACGCA203650.032.5397575
TATCAAC205500.032.391032
AACGCAG213200.031.5786366
ATAACGC6850.031.562083
ACGCAGA236300.028.412077
GTACATG161600.028.1311531
CGCAGAG243900.027.5062898
TAACGCA7700.027.4660824
TACATGG166800.026.914152
ACATGGG168550.025.4310593
GCAGAGT263650.025.2306049
Produced by FastQC (version 0.11.5)