FastQCFastQC Report
Mon 6 Jun 2016
ERR523133_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523133_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4643976
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178360.38406744565432727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154960.3336795883527391No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89360.19242132172948354No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53190.114535475635533No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94100.051.4966351
TACCTGG50250.047.264182
GTATCAA161200.039.4003451
ATAACGC6150.036.6792263
ACCTGGG63550.036.6792223
TATAACG6100.036.236382
GTACCTG70600.034.2522961
TAACGCA6650.033.9195634
TCAACGC198700.031.6674694
ATCAACG201850.031.0352633
CAACGCA202400.030.9260435
TATCAAC207050.030.3464622
AACGCAG211300.029.9344676
GTACATG156050.027.8874761
ACGCAGA236250.026.7731347
TACATGG161300.026.6203172
CCTGGGG58750.026.3959164
CGCAGAG244200.025.9634518
ACATGGG162150.025.7655143
GCAGAGT262750.023.7724369
Produced by FastQC (version 0.11.5)