FastQCFastQC Report
Mon 6 Jun 2016
ERR523132_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523132_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7345628
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT661380.9003723030896745No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229600.31256687651484666No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT192130.2615569424424978No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184290.25088392714686886No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC156840.2135147600722498No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC133660.18195857454257144No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA114370.15569805604095388No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103610.1410498870892999No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG270950.025.9970571
TACATGG276700.025.0292382
ACATGGG278450.024.1139133
CATGGGG185300.021.9218654
GAGTACT209800.020.59054612-13
AGAGTAC339250.019.74602510-11
GTACTTT227500.018.72188614-15
ATGGGGG105600.018.5214315
AGTACTT224000.018.28900512-13
GAGTACA217350.016.1391531
CATGGGA96500.016.077774
ACTTTTT279250.015.38557116-17
GTATAAT32100.015.3810431
AGTACAT214100.014.9988552
TACTTTT267950.014.93994414-15
GTATATA36400.014.7266861
TATAACG6100.014.6446092
TATAGCG2952.416513E-514.3438465
GTGTAGC37400.014.2071961
TATACTG34100.014.2012955
Produced by FastQC (version 0.11.5)