FastQCFastQC Report
Mon 6 Jun 2016
ERR523132_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523132_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7345628
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT658550.8965196712929107No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202720.27597368121554755No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT186230.2535249538909403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171080.23290044091533085No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC155150.2112140718261257No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC140590.19139275770567202No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG108750.14804724660709745No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95600.1301454416150668No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG268800.026.5587161
TACATGG274750.025.4724182
ACATGGG278500.024.3661633
CATGGGG179100.023.0120374
GAGTACT199350.020.82328812-13
ATGGGGG102750.019.163615
AGAGTAC329950.019.05682210-11
GTACTTT216100.018.85811214-15
AGTACTT211250.017.9589812-13
GAGTACA214950.016.7374211
AGTACAT208950.015.8134332
ACTTTTT264550.015.48168116-17
TATACTG33200.015.4288515
CATGGGA98150.015.4175954
TACCTGG48400.015.0521842
TACTTTT251600.014.90823114-15
GTATAGG20450.014.4881121
ATGGGGA77300.014.4083435
GTATATA35200.014.2957081
CATGGGT53650.014.2780514
Produced by FastQC (version 0.11.5)