FastQCFastQC Report
Mon 6 Jun 2016
ERR523131_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523131_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22131359
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1218420.5505400730248874No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT595710.2691700947962572No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT473210.2138187718160462No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT337220.15237202559499397No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC277420.12535154302996035No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262010.11838857252281706No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC242460.10955495322270992No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG824250.025.134671
TACATGG839450.024.1418672
ACATGGG862500.022.8475783
CATGGGG545050.021.9427624
TACCTGG206050.021.8438152
GAGTACT531800.020.13688312-13
GTACCTG247600.018.918861
GTACTTT565600.018.67669514-15
TATAACG14600.018.0394942
AGTACTT563850.017.86699312-13
AGAGTAC968200.017.83372510-11
ACCTGGG257100.016.7197343
ATGGGGG297700.016.6344055
GAGTACA683250.015.6392141
TACTTTT641700.015.63413414-15
ACTTTTT696350.015.41699916-17
GTATAGG53000.015.35171
GTATATA91950.015.2934591
AGTACAT651100.015.0174422
TATACTG93700.014.7057035
Produced by FastQC (version 0.11.5)