FastQCFastQC Report
Mon 6 Jun 2016
ERR523131_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523131_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22131359
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1247590.5637204656071956No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT566530.2559851837386036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT483850.2186264295834702No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT335080.15140507187109475No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC287810.1300462389137513No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263930.11925611978911915No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC249160.11258233170407655No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG823200.023.8442691
TACCTGG206150.023.0060772
TACATGG835850.022.9663542
TATAACG16900.022.250412
ACATGGG850900.021.780373
CATGGGG541650.021.0431164
GTACCTG244500.019.8537181
GAGTACT544050.019.57482712-13
GTACTTT569600.018.50260514-15
ACCTGGG259850.017.8349233
AGAGTAC986700.017.6689610-11
ATAACGC23200.017.6257783
AGTACTT571750.017.49211112-13
ATGGGGG284450.016.4084385
TAACGCA22900.016.0094414
TATACTG92600.015.4307325
GAGTACA691350.015.3422691
TACTTTT648700.015.28290914-15
GTATATA90450.015.23702051
ACTTTTT697450.015.17149416-17
Produced by FastQC (version 0.11.5)