FastQCFastQC Report
Mon 6 Jun 2016
ERR523130_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523130_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14537771
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT414320.2849955471165421No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344120.2367075392782016No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT203750.14015215950230608No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190410.13097606228630235No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG175400.038.444222
GTACCTG208800.033.0222321
ACCTGGG217150.030.7066463
GTACATG676100.022.3304271
GTATCAA785900.022.0472951
TACATGG689300.021.563922
CATGGGG395350.021.062184
CCTGGGG217900.021.0168784
ACATGGG704050.020.5179373
GAGTACT377150.020.47085412-13
TCAACGC875300.019.5743774
CAACGCA879750.019.4155715
ATCAACG889200.019.2600083
GGTATCA608200.019.2555471
TATCAAC897100.019.1111722
AACGCAG899200.019.1063026
GTACTTT415250.018.5303114-15
ATGGGGG217000.018.156345
AGTACTT407550.017.7620612-13
GTATAGG42800.017.6923311
Produced by FastQC (version 0.11.5)