##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR523130_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14537771
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.143618371757267	33.0	31.0	34.0	27.0	34.0
2	31.489408039237926	34.0	31.0	34.0	27.0	35.0
3	31.444949435508374	33.0	31.0	34.0	26.0	36.0
4	33.73387398934816	35.0	33.0	37.0	30.0	37.0
5	33.817151817840575	35.0	34.0	37.0	30.0	37.0
6	34.047418754910915	35.0	35.0	37.0	30.0	37.0
7	33.91696698207724	35.0	34.0	37.0	30.0	37.0
8	34.00354284023321	35.0	34.0	37.0	30.0	37.0
9	35.15717113717089	37.0	34.0	39.0	29.0	39.0
10-11	35.121175660285196	37.0	34.5	39.0	30.0	39.0
12-13	35.14891859969455	37.0	35.0	39.0	29.5	39.0
14-15	35.89502617010544	38.0	35.0	40.0	30.0	41.0
16-17	36.05613625362513	38.0	36.0	40.0	29.0	41.0
18-19	35.909246334943646	38.0	35.0	40.0	28.5	41.0
20-21	36.00260669259407	38.0	35.0	40.0	28.5	41.0
22-23	35.85618256058649	38.0	35.0	40.0	28.5	41.0
24-25	35.8244687235753	37.5	35.0	40.0	27.5	41.0
26-27	35.70807330092075	37.0	35.5	40.0	27.0	41.0
28-29	35.47610782285675	37.5	34.5	40.0	27.0	41.0
30-31	35.304753699862246	37.0	34.5	40.0	27.0	41.0
32-33	35.28776890900262	37.5	34.0	40.0	26.5	41.0
34-35	35.14226207717813	37.5	34.0	40.0	26.0	41.0
36-37	34.96975640213345	37.5	34.0	40.0	26.0	41.0
38-39	34.806896050295464	36.5	34.0	40.0	25.0	41.0
40-41	34.75802256755867	37.0	33.0	40.0	24.5	41.0
42-43	34.554514443789216	36.0	34.0	40.0	24.0	41.0
44-45	34.46674032766096	37.0	33.0	40.0	24.0	41.0
46-47	34.26376268411437	36.5	33.0	40.0	23.0	41.0
48-49	34.128401114586275	37.0	33.0	40.0	22.5	41.0
50-51	33.12029536715085	36.0	31.5	38.5	21.5	40.0
52-53	33.34952301147129	36.5	32.0	38.5	22.0	39.5
54-55	34.20483050668497	37.0	33.0	39.5	23.0	40.5
56-57	34.343524292685586	37.0	33.0	40.0	23.5	41.0
58-59	34.26645260817494	37.0	33.0	40.0	23.0	41.0
60-61	34.01361790607377	36.5	33.0	39.0	23.0	41.0
62-63	33.839499019485174	36.0	32.5	39.0	22.5	41.0
64-65	33.44486565375118	36.0	33.0	39.0	22.0	41.0
66-67	33.24567387256272	36.0	32.5	38.0	21.5	40.5
68-69	32.90257705256191	35.0	32.0	37.5	20.5	40.0
70-71	32.685753957742214	35.0	31.5	37.0	20.0	40.0
72-73	32.35930700793127	35.0	31.0	37.0	20.0	39.0
74-75	31.991350359006205	35.0	31.0	36.5	20.0	39.0
76-77	31.867787193786448	35.0	31.0	37.0	19.0	38.5
78-79	31.595139722588833	35.0	30.5	36.0	18.0	38.0
80-81	31.329085421692227	35.0	30.0	36.0	18.0	37.5
82-83	31.021921998908912	34.0	30.0	35.5	18.0	37.0
84-85	30.786146652055532	34.0	29.5	35.0	17.5	37.0
86-87	30.54850334346304	34.0	29.0	35.0	17.0	37.0
88-89	30.387483198077614	34.0	29.0	35.0	17.5	36.5
90-91	30.17043307395611	34.0	29.0	35.0	17.0	36.0
92-93	29.919144172789625	34.0	29.0	35.0	16.5	36.0
94-95	29.823425234858906	34.0	29.0	35.0	16.0	36.0
96-97	29.635577145904968	34.0	27.5	35.0	16.0	36.0
98-99	29.551787581466236	34.0	27.0	35.0	15.0	36.0
100	28.29897409994971	33.0	25.0	35.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.06435100563518503
1101	2	0.04869335344758241
1101	3	0.1082513967070291
1101	4	0.09735644553801137
1101	5	0.05015600001328124
1101	6	0.16279721134353764
1101	7	0.13543670792006424
1101	8	0.10190110046971057
1101	9	0.17941746632806854
1101	10-11	0.11570814566945131
1101	12-13	0.11982274713857777
1101	14-15	-0.03190326582944181
1101	16-17	0.10844475716870505
1101	18-19	0.039723487198905616
1101	20-21	0.07586709526838575
1101	22-23	0.09922434366222177
1101	24-25	-0.027096336069575955
1101	26-27	-0.010659570848645217
1101	28-29	-0.04595165147230773
1101	30-31	-0.08128023704636433
1101	32-33	-0.13929424667742296
1101	34-35	-0.1383838831016675
1101	36-37	-0.12316920873065129
1101	38-39	-0.19012186659098518
1101	40-41	-0.18035651797978147
1101	42-43	-0.18957415464252136
1101	44-45	-0.14958408974293036
1101	46-47	-0.13706044062953282
1101	48-49	-0.20903981576287833
1101	50-51	-0.29007189827046886
1101	52-53	-0.20589776118312386
1101	54-55	-0.1293613084050591
1101	56-57	-0.12555998595875195
1101	58-59	-0.13882536964938907
1101	60-61	-0.1344935749855054
1101	62-63	-0.21058786541633623
1101	64-65	-0.23416043044618107
1101	66-67	-0.24548944919899185
1101	68-69	-0.1851583552415903
1101	70-71	-0.327338556477784
1101	72-73	-0.24601155812405295
1101	74-75	-0.20944612378903216
1101	76-77	-0.18074262476892144
1101	78-79	-0.3490737602093681
1101	80-81	-0.3204277143462342
1101	82-83	-0.47530889275865107
1101	84-85	-0.4306868211978703
1101	86-87	-0.41606397081536883
1101	88-89	-0.5241756684591508
1101	90-91	-0.4555039607880964
1101	92-93	-0.46149982177806237
1101	94-95	-0.4525650132386083
1101	96-97	-0.45320860372440563
1101	98-99	-0.5056658722326794
1101	100	-0.5061881493825879
1102	1	0.10225959780734328
1102	2	0.03822415450938976
1102	3	0.05915146873113031
1102	4	0.041172045845854655
1102	5	0.06490700895033541
1102	6	0.140271558488422
1102	7	0.06506762495408225
1102	8	0.18789887590546783
1102	9	0.1362321033752636
1102	10-11	0.15334882987020393
1102	12-13	0.11411090421744063
1102	14-15	0.014370280444111927
1102	16-17	0.12074676583699073
1102	18-19	0.09355506719327167
1102	20-21	0.030290687288875517
1102	22-23	0.07231049358946251
1102	24-25	0.011305459841914
1102	26-27	0.03497618118245782
1102	28-29	-6.335179758778509E-4
1102	30-31	-0.08960275530105832
1102	32-33	0.0028367188547093747
1102	34-35	-0.09932196177230423
1102	36-37	-0.4956790512017406
1102	38-39	-0.16080996131008618
1102	40-41	-0.8100346457199308
1102	42-43	-0.7899595118108138
1102	44-45	-0.25532661433235404
1102	46-47	-0.18606678929673848
1102	48-49	-0.24086533402250154
1102	50-51	-0.2828215189270651
1102	52-53	-0.10630129191611104
1102	54-55	8.454487396747368E-4
1102	56-57	0.04825924818025129
1102	58-59	0.032230023305231725
1102	60-61	-0.07270541702487066
1102	62-63	-0.26646439028510827
1102	64-65	-0.09318281611584922
1102	66-67	0.005415988479704481
1102	68-69	0.01845218800073667
1102	70-71	-0.09464169030223957
1102	72-73	-0.06253308758162746
1102	74-75	-0.0511824601959141
1102	76-77	-0.18111841899161263
1102	78-79	-0.2012568100668375
1102	80-81	-0.2255088878382594
1102	82-83	-0.26270415447561746
1102	84-85	-0.3960824714287945
1102	86-87	-0.30775845335287855
1102	88-89	-0.34918893048791944
1102	90-91	-0.3190000987512889
1102	92-93	-0.36717630957664227
1102	94-95	-0.4731950733551038
1102	96-97	-0.6834556980703361
1102	98-99	-0.6124729303478773
1102	100	-0.6048554830305441
1103	1	0.06048099824630171
1103	2	0.056351374261392806
1103	3	0.04073064644475721
1103	4	0.05928956969921728
1103	5	0.14870870557174243
1103	6	0.08969238939834412
1103	7	0.13222669135431886
1103	8	0.09463541202200076
1103	9	0.1159436365394555
1103	10-11	0.05361737498542851
1103	12-13	0.12773940572702713
1103	14-15	0.07143982599733789
1103	16-17	-0.013966374299535289
1103	18-19	0.027287062814302487
1103	20-21	-0.09031433946710621
1103	22-23	-0.04439986494119097
1103	24-25	-0.09158243452480264
1103	26-27	-0.07022273285902259
1103	28-29	-0.26481360218006955
1103	30-31	-0.22686846362868351
1103	32-33	-0.273794842850279
1103	34-35	-0.2746946071043368
1103	36-37	-0.4844114563292692
1103	38-39	-0.2558182848145947
1103	40-41	-0.22115531421683698
1103	42-43	-0.23270674951855597
1103	44-45	-0.2548743699172462
1103	46-47	-0.19249758289888774
1103	48-49	-0.24727619964865255
1103	50-51	-0.36044614618789694
1103	52-53	-0.19842392385483976
1103	54-55	-0.09461983308457178
1103	56-57	0.08715396577871815
1103	58-59	0.096297505508673
1103	60-61	0.010474513340703595
1103	62-63	-0.017740216394663832
1103	64-65	-0.16562949839157426
1103	66-67	-0.06834754874854099
1103	68-69	-0.14552645832402078
1103	70-71	-0.11133367130703675
1103	72-73	-0.12528110096126
1103	74-75	-0.09968798040165083
1103	76-77	-0.10847113884980075
1103	78-79	-0.18718940158796826
1103	80-81	-0.11888708950106874
1103	82-83	-0.11022477691477306
1103	84-85	-0.09323029550158068
1103	86-87	-0.8273798244896717
1103	88-89	-0.3871461172421391
1103	90-91	-0.5656409055179701
1103	92-93	-0.21207600103706525
1103	94-95	-0.613636635302715
1103	96-97	-0.3256601263078842
1103	98-99	-0.3307095350282623
1103	100	-0.3010660443484561
1104	1	0.04119835487836809
1104	2	-0.015014767552205655
1104	3	0.02799476697123282
1104	4	0.08278742576065667
1104	5	0.0041071102143845906
1104	6	0.04640643609859296
1104	7	0.004718394910085522
1104	8	0.10443678008872581
1104	9	0.022202012237613644
1104	10-11	-0.05046963248749847
1104	12-13	0.004160565070151279
1104	14-15	-0.0908873209072425
1104	16-17	-0.04179568213423579
1104	18-19	0.005797160303828264
1104	20-21	-0.025632605189649382
1104	22-23	-0.021376489178187796
1104	24-25	-0.025288183219757343
1104	26-27	-0.0267381494272243
1104	28-29	-0.04182441817840754
1104	30-31	-0.0869955878138029
1104	32-33	-0.04335491245683443
1104	34-35	-0.13643911162564848
1104	36-37	-0.1161761835084647
1104	38-39	-0.046115052011252544
1104	40-41	-0.05639792396910792
1104	42-43	-0.2534845375633168
1104	44-45	-0.14114975578775812
1104	46-47	-0.09101143234094167
1104	48-49	-0.07475982080580224
1104	50-51	-0.2728803459226583
1104	52-53	-0.1700190794711105
1104	54-55	-0.04757770188187038
1104	56-57	-0.08158134823010954
1104	58-59	-0.10569505657115741
1104	60-61	-0.19051135886787307
1104	62-63	-0.07998057725696839
1104	64-65	-0.139483840977924
1104	66-67	-0.01627230591934392
1104	68-69	-0.11933021649678466
1104	70-71	-0.06201949688789199
1104	72-73	-0.03383951392076057
1104	74-75	-0.06181245792619805
1104	76-77	-0.11595150789446862
1104	78-79	-0.17303763886074464
1104	80-81	-0.09533247232390707
1104	82-83	-0.07986633846192959
1104	84-85	-0.07651008290654815
1104	86-87	-0.1943418936245429
1104	88-89	-0.15463591089856266
1104	90-91	-0.20439821489068422
1104	92-93	-0.15955689874139267
1104	94-95	-0.16186048295074684
1104	96-97	-0.2650264585110129
1104	98-99	-0.30006431897487573
1104	100	-0.14561972110582744
1105	1	0.13278218767505834
1105	2	0.10466164442453518
1105	3	0.08269160125634656
1105	4	0.09459359569147807
1105	5	0.11171757435933927
1105	6	0.21065475165565317
1105	7	0.0633762686801731
1105	8	0.003425022686478485
1105	9	0.13290050312102153
1105	10-11	0.09714739967180464
1105	12-13	0.20288598228105315
1105	14-15	0.1510324413406039
1105	16-17	0.09185195951907588
1105	18-19	0.08464114745795115
1105	20-21	0.07534881578597918
1105	22-23	0.0869784929727544
1105	24-25	0.1249065157289806
1105	26-27	0.113952783849399
1105	28-29	-0.03677773409618368
1105	30-31	-0.026454551686008188
1105	32-33	-0.02490390819850319
1105	34-35	-0.023951829589123008
1105	36-37	-0.02817655798732943
1105	38-39	-0.06724240543766768
1105	40-41	-0.04140995926409374
1105	42-43	-0.09461942486527164
1105	44-45	-0.05581550373927513
1105	46-47	-0.09023477828980475
1105	48-49	0.019077579348987683
1105	50-51	-0.08586666810990806
1105	52-53	0.028214240952429748
1105	54-55	0.10230142110351181
1105	56-57	0.134331266407564
1105	58-59	0.08522561284263475
1105	60-61	0.11684397672024005
1105	62-63	0.13649489279548277
1105	64-65	0.1364307053821463
1105	66-67	0.18890859015304073
1105	68-69	0.16186931508030966
1105	70-71	0.16287268025342883
1105	72-73	0.10993874404533699
1105	74-75	0.15320194930556497
1105	76-77	0.21963471052400863
1105	78-79	0.17042262255619178
1105	80-81	0.20319353924364592
1105	82-83	0.14013926131810095
1105	84-85	0.14093743823956117
1105	86-87	0.12514105144999021
1105	88-89	0.06171634461277975
1105	90-91	0.025477659684366216
1105	92-93	0.05754827958775621
1105	94-95	0.12663062827849458
1105	96-97	0.10289013419331283
1105	98-99	0.15104325921479145
1105	100	0.02941279859425805
1106	1	0.11495828383592155
1106	2	0.11389690478061709
1106	3	0.17590811290672548
1106	4	0.19074582877490798
1106	5	0.1164439216339872
1106	6	0.1750203170643303
1106	7	0.20031802784752273
1106	8	-0.9885109725183483
1106	9	0.15595278354117426
1106	10-11	0.12336289412878187
1106	12-13	0.13233781572936465
1106	14-15	0.08719325607595607
1106	16-17	0.07524500659285849
1106	18-19	0.15600751959834014
1106	20-21	0.14105701148279337
1106	22-23	0.11632802230587203
1106	24-25	0.1610921945883419
1106	26-27	0.07702258005862461
1106	28-29	0.02459673786466965
1106	30-31	0.04996084230219111
1106	32-33	0.08310858018678147
1106	34-35	0.03911653494677836
1106	36-37	0.1037395822343754
1106	38-39	0.07323826524733335
1106	40-41	0.11649072277477757
1106	42-43	0.11450318272235194
1106	44-45	-0.006650960419527507
1106	46-47	0.07760577709192518
1106	48-49	0.0025909889712636414
1106	50-51	0.12742178474326238
1106	52-53	0.14880297380545215
1106	54-55	0.2055981591702576
1106	56-57	0.16135692639705468
1106	58-59	0.1918929049846838
1106	60-61	0.17162458269779535
1106	62-63	0.1009279074604521
1106	64-65	0.033613153711648636
1106	66-67	0.11849825075324105
1106	68-69	0.13165385453741152
1106	70-71	0.14276768808102247
1106	72-73	0.11322242548021677
1106	74-75	0.18151391820313378
1106	76-77	0.1654418078719324
1106	78-79	0.1705700625079345
1106	80-81	0.13556843115564376
1106	82-83	0.2251707121878468
1106	84-85	0.17104990313663748
1106	86-87	0.08850472910876306
1106	88-89	0.16971146371246348
1106	90-91	0.13905859233889473
1106	92-93	0.11992186078472145
1106	94-95	0.054225539974275705
1106	96-97	0.036990023260553784
1106	98-99	0.14023918468016205
1106	100	0.12683074240979408
1107	1	0.005587263998375391
1107	2	-0.024861319845264518
1107	3	0.008645203971138216
1107	4	0.1636994200804054
1107	5	0.053169010033073505
1107	6	0.08683928258089679
1107	7	0.17193798657226012
1107	8	0.20028434723031552
1107	9	0.21139724954132078
1107	10-11	0.11283955411035862
1107	12-13	0.1345574534377434
1107	14-15	0.09263963456489677
1107	16-17	0.1780088553445509
1107	18-19	0.12392340339874863
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2313	1	-0.00913422396679664
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2314	96-97	0.4014972026596588
2314	98-99	0.46053829181655104
2314	100	0.39897935946142127
2315	1	-0.1114796201846282
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2315	3	-0.13777354737653624
2315	4	-0.1152164042374153
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2315	26-27	-0.15022683116542623
2315	28-29	-0.05034578022831937
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2315	100	0.37385526369402555
2316	1	-0.17559511765938396
2316	2	-0.36448759511036855
2316	3	-0.30167306848337816
2316	4	-0.27826954373636426
2316	5	-0.2652345544978587
2316	6	-0.20412330881436702
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2316	9	-0.16026143205436938
2316	10-11	-0.3177729283280186
2316	12-13	-0.2517904621899518
2316	14-15	-0.3294536302429094
2316	16-17	-0.45183218278298654
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2316	52-53	-0.033940425373415906
2316	54-55	-0.12887701912506344
2316	56-57	-0.24174919805118122
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2316	60-61	-0.20855808701276857
2316	62-63	-0.1794655789298858
2316	64-65	-0.39289374169913316
2316	66-67	-0.3571243378907667
2316	68-69	-0.17027393236003974
2316	70-71	-0.08904862965846405
2316	72-73	-0.18976198875684958
2316	74-75	-0.1177169136139149
2316	76-77	-0.23621889028786924
2316	78-79	-0.34884677856806334
2316	80-81	-0.14659268326581198
2316	82-83	-0.09501368107555308
2316	84-85	0.11554570237863615
2316	86-87	0.019315803158011846
2316	88-89	0.09796260398888279
2316	90-91	0.193292639494981
2316	92-93	0.28952283572125026
2316	94-95	0.31770122485212227
2316	96-97	0.3826117021491662
2316	98-99	0.4183378914979663
2316	100	0.08513194146530267
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	26406.0
1	2398.0
2	1507.0
3	1140.0
4	1026.0
5	938.0
6	992.0
7	1646.0
8	3104.0
9	5764.0
10	11349.0
11	23093.0
12	37847.0
13	50132.0
14	57713.0
15	64606.0
16	73420.0
17	85233.0
18	98714.0
19	114308.0
20	132210.0
21	152315.0
22	172424.0
23	187090.0
24	198767.0
25	212039.0
26	231156.0
27	259686.0
28	298417.0
29	349763.0
30	419772.0
31	514663.0
32	648933.0
33	857319.0
34	1224961.0
35	1924897.0
36	1979780.0
37	1991248.0
38	1756143.0
39	364608.0
40	244.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.44511539977856	17.877423052979417	12.94687151106996	23.73059003617207
2	16.17113513177894	19.965696538753637	39.331856904578295	24.531311424889125
3	19.45927124700377	25.124701895071343	29.200630611688783	26.215396246236107
4	13.340449763443102	16.044136587156547	36.637031358408976	33.97838229099138
5	14.05348050554901	36.769105151259865	33.85789460936747	15.319519733823661
6	31.85699173079044	35.208676487150456	17.983035916691477	14.95129586536763
7	28.47425738443039	31.734955355649593	21.51001459006269	18.28077266985733
8	26.739881065109078	33.8096490009654	20.144543700151548	19.305926233773974
9	27.179963527687484	15.808848608726606	19.996487820732078	37.014700042853825
10-11	25.43979419426013	25.42128569007137	27.769148750862154	21.369771364806343
12-13	26.083367471217283	23.13459912469086	27.788189257669988	22.993844146421864
14-15	23.571447704206292	24.608692465625897	25.613173637955654	26.20668619221216
16-17	23.053796259495854	27.463943816037954	26.317942438633953	23.16431748583224
18-19	22.963108473440613	26.676015197638396	27.99261606736349	22.368260261557495
20-21	23.684497360162215	25.803681169869762	27.907155087086288	22.60466638288173
22-23	23.556467474418223	25.578043794976168	27.64234246457029	23.223146266035318
24-25	23.38817078109574	25.7573265716755	27.64163116927321	23.212871477955552
26-27	23.15409735095497	25.761813434241194	27.916048904619196	23.168040310184644
28-29	23.055019986500383	26.0254147942343	27.91815750069179	23.001407718573525
30-31	23.59353764363952	25.758685601398383	27.681974425010946	22.965802329951146
32-33	23.214106104736114	25.980044895072485	27.70775108416314	23.098097916028262
34-35	23.494891415868675	25.967490264110143	27.655170915890736	22.882447404130442
36-37	23.606422595337456	25.83495211608387	27.545407946527263	23.013217342051412
38-39	23.408139389110694	26.073275796083735	27.547353485575943	22.971231329229624
40-41	23.356797737033222	26.03511511827569	27.546191956223232	23.061895188467858
42-43	23.264592658957177	26.061238523759034	27.27953577012321	23.39463304716058
44-45	23.259340154797638	26.037439600744282	27.330851315311772	23.372368929146305
46-47	23.394936828434737	26.011929284804864	27.231876378381937	23.361257508378458
48-49	23.39304216085456	26.129441226191663	27.171120750736904	23.30639586221687
50-51	23.16799490299258	26.3561413268044	27.207225035601308	23.268638734601712
52-53	23.204839106501826	26.496535650635106	26.889088924478443	23.40953631838463
54-55	23.062263935699605	26.26378244446501	27.055832875476128	23.618120744359253
56-57	23.28435747331599	26.280508327287073	26.834968812330512	23.600165387066426
58-59	23.334007713730106	26.12804949223363	27.09879173952866	23.439151054507604
60-61	23.41027894310581	26.2788206439405	27.20230793733221	23.108592475621485
62-63	23.076382436098058	26.357954194320172	27.255459612825195	23.31020375675658
64-65	23.05050040522509	26.272127803806917	27.154963991831632	23.52240779913636
66-67	23.176512028573402	26.185146910522377	26.91961503102489	23.718726029879328
68-69	22.965025597818432	26.215652804292784	27.055234348204483	23.76408724968431
70-71	23.11527934341752	26.064297836059076	27.041194943853768	23.779227876669637
72-73	23.040240383205603	26.20235587960831	26.95172499443717	23.805678742748917
74-75	22.942235703373317	26.487807260437762	26.84767737911854	23.722279657070384
76-77	22.920566547295756	26.141077120831756	26.95613011310673	23.982226218765764
78-79	22.94893980079348	26.084060353647825	27.01781634618084	23.949183499377856
80-81	22.981611447259098	26.339822589912508	26.7174519830164	23.961113979811994
82-83	23.014993981666233	26.14034069414818	26.86884860709915	23.975816717086442
84-85	22.963188334194363	26.27261042116625	26.721902572438232	24.042298672201156
86-87	22.862971234876188	26.290996827649682	26.877006128487896	23.969025808986235
88-89	22.768091997475175	26.03974530992926	26.91345228527771	24.278710407317856
90-91	23.088850562680737	25.967335424700355	26.845209466370306	24.098604546248602
92-93	23.03471769512615	26.005724529147045	26.859259031926637	24.100298743800167
94-95	22.859642521834903	25.814094233131357	27.008171423192902	24.31809182184084
96-97	22.787253756085395	25.76049485521426	27.154444558490965	24.297806830209378
98-99	22.90285794054435	25.782793014953832	26.883760977737904	24.430588066763917
100	22.934503516205453	25.51322664451763	26.92802851044514	24.62424132883178
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10625.0
1	12385.0
2	11904.0
3	7154.0
4	3841.5
5	3342.5
6	4497.5
7	7227.0
8	11504.0
9	14306.5
10	13096.5
11	11480.5
12	11906.0
13	12670.5
14	13541.5
15	14281.5
16	15128.0
17	16211.0
18	17286.5
19	18753.0
20	20184.0
21	22143.0
22	25105.5
23	29370.5
24	35215.0
25	42520.0
26	52436.0
27	64608.0
28	78330.5
29	96037.0
30	118137.5
31	138667.5
32	160981.0
33	187730.0
34	213641.5
35	240452.5
36	278441.0
37	313764.0
38	336643.0
39	360061.5
40	379292.0
41	395310.0
42	414347.0
43	440139.0
44	467980.5
45	495537.0
46	522262.0
47	556873.5
48	607193.5
49	631599.5
50	632010.0
51	616293.0
52	587142.5
53	572513.5
54	555719.5
55	525459.0
56	481254.5
57	432423.0
58	389259.5
59	344455.0
60	296716.0
61	251022.5
62	208025.5
63	165507.0
64	125742.0
65	94780.5
66	71379.5
67	54377.0
68	42456.0
69	33031.0
70	25920.0
71	20102.0
72	15638.5
73	12445.5
74	9731.0
75	7177.0
76	5132.5
77	3457.5
78	2250.5
79	1563.0
80	1056.0
81	678.5
82	506.5
83	391.5
84	261.0
85	208.5
86	174.5
87	128.0
88	99.5
89	86.5
90	72.5
91	52.5
92	46.5
93	48.0
94	40.0
95	31.0
96	26.5
97	21.5
98	14.5
99	7.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14666622551696543
2	0.07135894491665881
3	0.31511020499635056
4	0.31288152771150407
5	0.3145186425071629
6	0.26192461003822387
7	0.26259871613055397
8	0.2618902168702478
9	0.2727378220498865
10-11	0.24989387988020995
12-13	0.2596752968525918
14-15	0.23294836601842195
16-17	0.24162576229877333
18-19	0.2551766704813276
20-21	0.2569410399984977
22-23	0.24758609830901865
24-25	0.24764456669457788
26-27	0.24644768444901216
28-29	0.25697543316647375
30-31	0.24296021721624314
32-33	0.2549943866910546
34-35	0.23890182339507204
36-37	0.2467812981783796
38-39	0.24827052235174155
40-41	0.26533985161824325
42-43	0.2511182766601565
44-45	0.23651837685433344
46-47	0.23941772091471245
48-49	0.24959809863561613
50-51	0.24173925975309418
52-53	0.25845433938944284
54-55	0.26876885046545307
56-57	0.26449034036923547
58-59	0.26167353991199893
60-61	0.2427985693267558
62-63	0.2703784507267311
64-65	0.2642908599949745
66-67	0.2700001258789948
68-69	0.27482548734603124
70-71	0.2950761846503154
72-73	0.30435202205344963
74-75	0.28306952970988464
76-77	0.28064481136757485
78-79	0.2775803801009109
80-81	0.2639331710480238
82-83	0.2750593608882682
84-85	0.285628381407301
86-87	0.27841613408272836
88-89	0.2869834722255564
90-91	0.28115383025362
92-93	0.2606211089719325
94-95	0.2818141790787597
96-97	0.28039374124134986
98-99	0.26628222442078636
100	0.28911584864007006
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.4537771E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.47435555870755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.27182842930486	32.41161057495898
2	16.05348048753751	15.242572812402594
3	6.492714843298505	9.247123590361543
4	3.128496882516852	5.940934934596527
5	1.7666281308541865	4.193476601209329
6	1.0646891441344737	3.032725859288162
7	0.6836849029363169	2.272025012048342
8	0.4769786746398912	1.8115404155020225
9	0.3554079759752708	1.518548815786554
>10	1.572225935676324	12.755120943883616
>50	0.08168146547794528	2.6345281978948125
>100	0.04600557520181404	4.211445933603384
>500	0.004118924965159163	1.2962543162990412
>1k	0.0018135528158738118	1.5876215712216206
>5k	1.22537332530724E-4	0.35747805592273013
>10k+	1.22537332530724E-4	1.4869923650206818
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41432	0.2849955471165421	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34412	0.2367075392782016	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	20375	0.14015215950230608	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19041	0.13097606228630235	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	6.878633595205207E-6	0.0	0.0	0.0	0.0
3	6.878633595205207E-6	0.0	0.0	1.3757267190410414E-5	0.0
4	6.878633595205207E-6	0.0	0.0	1.3757267190410414E-5	1.3757267190410414E-5
5	6.878633595205207E-6	0.0	0.0	1.3757267190410414E-5	1.3757267190410414E-5
6	2.0635900785615622E-5	0.0	0.0	1.3757267190410414E-5	4.1271801571231243E-5
7	2.0635900785615622E-5	0.0	0.0	1.3757267190410414E-5	4.1271801571231243E-5
8	2.0635900785615622E-5	0.0	0.0	1.3757267190410414E-5	4.1271801571231243E-5
9	4.1271801571231243E-5	0.0	0.0	2.0635900785615622E-5	6.190770235684687E-5
10-11	4.4711118368833845E-5	0.0	0.0	2.4075217583218223E-5	6.534701915444947E-5
12-13	4.815043516643645E-5	0.0	0.0	2.7514534380820828E-5	7.910428634485989E-5
14-15	4.815043516643645E-5	0.0	0.0	4.1271801571231243E-5	1.5476925589211714E-4
16-17	4.815043516643645E-5	0.0	0.0	6.878633595205208E-5	2.0291969105855362E-4
18-19	4.815043516643645E-5	0.0	0.0	7.566496954725728E-5	2.3043422543937443E-4
20-21	4.815043516643645E-5	0.0	0.0	9.974018713047551E-5	2.441914926297849E-4
22-23	4.815043516643645E-5	0.0	0.0	2.957812445938239E-4	3.060991949866317E-4
24-25	4.815043516643645E-5	0.0	0.0	6.809847259253154E-4	3.5768894695067076E-4
26-27	4.815043516643645E-5	0.0	0.0	0.0014169985206122725	3.8176416453388905E-4
28-29	4.815043516643645E-5	0.0	0.0	0.004354175065764896	3.852034813314916E-4
30-31	4.815043516643645E-5	0.0	0.0	0.013265444888353243	3.920821149266968E-4
32-33	4.815043516643645E-5	6.878633595205207E-6	0.0	0.03442412182720446	4.505505004859411E-4
34-35	4.815043516643645E-5	6.878633595205207E-6	0.0	0.06924376508613322	5.193368364379931E-4
36-37	4.815043516643645E-5	6.878633595205207E-6	0.0	0.12263915836891365	5.399727372236088E-4
38-39	5.5029068761641656E-5	6.878633595205207E-6	0.0	0.19937375544022534	5.778052219972375E-4
40-41	5.5029068761641656E-5	1.0317950392807811E-5	0.0	0.2858416190487524	6.121983899732635E-4
42-43	5.5029068761641656E-5	1.3757267190410414E-5	0.0	0.369344791577746	6.293949739612765E-4
44-45	5.8468385559244264E-5	1.3757267190410414E-5	0.0	0.4749077420465627	6.534701915444946E-4
46-47	9.63008703328729E-5	1.3757267190410414E-5	0.0	0.6020283302027525	7.222565274965468E-4
48-49	9.974018713047551E-5	1.3757267190410414E-5	0.0	0.7343938764752864	8.116787642342145E-4
50-51	1.0317950392807811E-4	1.3757267190410414E-5	0.0	0.8705942609771471	8.254360314246248E-4
52-53	1.0317950392807811E-4	1.3757267190410414E-5	0.0	1.0110284444568565	9.320548521503056E-4
54-55	1.1005813752328332E-4	1.3757267190410414E-5	0.0	1.172614426241822	0.0010386736728759862
56-57	1.6508720628492497E-4	1.3757267190410414E-5	0.0	1.3634483580736	0.0010489916232687942
58-59	2.1323764145136142E-4	1.3757267190410414E-5	0.0	1.5769714628191627	0.0010661882072568072
60-61	2.1323764145136142E-4	1.3757267190410414E-5	0.0	1.79292616454063	0.0010868241080424228
62-63	2.1323764145136142E-4	2.0635900785615622E-5	0.0	2.0118042855400597	0.00116248907758968
64-65	2.1667695824896402E-4	2.0635900785615622E-5	0.0	2.239727809717184	0.0011693677111848852
66-67	2.3731285903457963E-4	2.0635900785615622E-5	0.0	2.4982406174921863	0.0011831249783752955
68-69	2.6826671021300307E-4	2.0635900785615622E-5	0.0	2.7953459990530876	0.0011900036119705008
70-71	2.8890261099861873E-4	2.0635900785615622E-5	0.0	3.11422569525961	0.0012278360967441296
72-73	2.9578124459382393E-4	2.0635900785615622E-5	0.0	3.425731496252073	0.0014032412534218622
74-75	3.0953851178423434E-4	2.0635900785615622E-5	0.0	3.737811663149736	0.0014032412534218622
76-77	3.0953851178423434E-4	2.0635900785615622E-5	0.0	4.080054638362373	0.001406680570219465
78-79	3.129778285818369E-4	2.0635900785615622E-5	0.0	4.465123986338758	0.0014101198870170675
80-81	3.1985646217704217E-4	2.0635900785615622E-5	0.0	4.870389002550666	0.0014513916885882986
82-83	3.2329577897464474E-4	2.4075217583218223E-5	0.0	5.285321938280635	0.0015408139253259665
84-85	3.2329577897464474E-4	2.7514534380820828E-5	0.0	5.707198854624963	0.0015408139253259665
86-87	3.5768894695067076E-4	2.7514534380820828E-5	0.0	6.140483984786939	0.0015511318757187743
88	3.7144621414108116E-4	2.7514534380820828E-5	0.0	6.486510208476939	0.001561449826111582
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	17540	0.0	38.44422	2
GTACCTG	20880	0.0	33.022232	1
ACCTGGG	21715	0.0	30.706646	3
GTACATG	67610	0.0	22.330427	1
GTATCAA	78590	0.0	22.047295	1
TACATGG	68930	0.0	21.56392	2
CATGGGG	39535	0.0	21.06218	4
CCTGGGG	21790	0.0	21.016878	4
ACATGGG	70405	0.0	20.517937	3
GAGTACT	37715	0.0	20.470854	12-13
TCAACGC	87530	0.0	19.574377	4
CAACGCA	87975	0.0	19.415571	5
ATCAACG	88920	0.0	19.260008	3
GGTATCA	60820	0.0	19.255547	1
TATCAAC	89710	0.0	19.111172	2
AACGCAG	89920	0.0	19.106302	6
GTACTTT	41525	0.0	18.53031	14-15
ATGGGGG	21700	0.0	18.15634	5
AGTACTT	40755	0.0	17.76206	12-13
GTATAGG	4280	0.0	17.692331	1
>>END_MODULE