FastQCFastQC Report
Mon 6 Jun 2016
ERR523130_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523130_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14537771
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380770.26191773140462865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341420.2348503082074962No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT217200.1494039216878571No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191590.13178774105053656No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG180050.038.553272
GTACCTG211650.033.2120321
ACCTGGG213550.031.6436673
CCTGGGG209550.021.3489654
GTACATG687000.020.5801851
GTATCAA801350.020.5243841
CATGGGG387400.020.2938214
TACATGG704800.019.8045232
GAGTACT391400.019.27593612-13
ACATGGG716800.018.9333153
GGTATCA634700.018.6905161
TCAACGC896450.018.0903264
TATAACG13000.018.0759222
ATGGGGG208750.018.054025
CAACGCA903900.017.961965
ATCAACG911900.017.7941343
TATCAAC923800.017.6380142
AACGCAG924450.017.6135676
AGTACTT421050.017.42173412-13
GTACTTT431800.017.42114814-15
Produced by FastQC (version 0.11.5)