FastQCFastQC Report
Mon 6 Jun 2016
ERR523129_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523129_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4295207
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147250.34282398962378297No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT120770.28117387590400184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119640.2785430364590112No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64310.14972503071446847No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG55800.043.4918252
ACCTGGG61000.038.0880933
GTACCTG76400.032.6268121
CCTGGGG57950.025.8889354
GTACATG163900.025.022441
TATAACG3300.024.2285332
TACATGG166950.024.1990812
CATGGGG105050.023.9516074
ACATGGG163400.023.6886273
ATGGGGG65750.022.7456425
GTATACG2501.065564E-820.6940171
GTATCAA281450.020.6709581
ATAACGC3950.020.2415583
CCGTATC12350.020.1670294
GAGTACT135900.020.02431712-13
TGGGGGG69450.019.4246776
TCAACGC308500.018.5681694
CAACGCA310800.018.430335
ATCAACG311750.018.3450643
CGCCGTA17700.018.31929294
Produced by FastQC (version 0.11.5)