FastQCFastQC Report
Mon 6 Jun 2016
ERR523129_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523129_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4295207
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140280.32659659941884056No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT120300.28007963294900573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119050.27716941232401604No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64950.1512150636744632No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT55270.1286783151545432No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG55350.045.2647322
ACCTGGG63400.037.9582183
GTACCTG78150.033.2253071
TATAACG3850.032.9650232
TAACGCA5150.028.2930374
CCTGGGG59450.026.8814164
ATAACGC5450.025.0107423
GTACATG156400.024.0909921
TACATGG159450.023.1417122
ACATGGG157150.022.5219333
ATGGGGG60300.022.0599385
CATGGGG99400.021.2317144
GAGTACT129100.020.85993812-13
TGGGGGG59700.019.838596
GTACTTT144850.018.5266614-15
GTATCAA285600.018.347881
AGTACTT136800.017.9680212-13
CTGGGGG36800.017.7542445
AGAGTAC211450.017.47036610-11
TATCACG3251.1426891E-817.3559442
Produced by FastQC (version 0.11.5)