FastQCFastQC Report
Mon 6 Jun 2016
ERR523128_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523128_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9015952
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255640.28354188221055304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193790.21494125079636628No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT116760.12950379505126025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110100.12211688793374233No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG121900.049.30542
ACCTGGG140650.041.3615953
GTACCTG170750.035.6127171
CCTGGGG130350.029.0435524
TATAACG9000.027.6949562
GTACATG296050.025.9569871
ATAACGC9850.025.782493
GTATCAA400250.025.344641
TACATGG301850.024.8350162
TAACGCA11800.023.9130064
CATGGGG189700.023.8243454
ACATGGG302850.023.6038763
GGTATCA293800.023.3865281
TATCACG8150.023.0818022
TCAACGC450150.022.2006954
CAACGCA454250.022.009695
ATCAACG455000.021.8711473
CCGTATC44500.021.85706794
TATCAAC460050.021.7537382
AACGCAG468900.021.4339336
Produced by FastQC (version 0.11.5)