FastQCFastQC Report
Mon 6 Jun 2016
ERR523128_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523128_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9015952
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246890.27383686159819837No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT243110.2696442926936612No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199370.22113028108401642No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT121790.13508279547184812RNA PCR Primer, Index 23 (95% over 23bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT118700.13165553676417088No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113480.12586579875314333No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG125300.048.392372
ACCTGGG141650.042.3411523
GTACCTG176900.034.7260061
CCTGGGG128050.030.8702534
GTACATG291200.024.5684261
TATAACG9000.024.5467262
GTATCAA409400.024.0469971
TACATGG296950.023.4586562
GGTATCA305500.022.6947671
TATCACG7750.022.4407962
ACATGGG296950.022.1443523
CATGGGG185200.021.8517384
TAACGCA9950.021.257554
TCAACGC458600.021.2260824
CAACGCA463300.021.0316265
TGGGGGG112850.020.7817366
ATCAACG468150.020.7631913
ATGGGGG112350.020.583915
AACGCAG479000.020.5458476
CTGGGGG80450.020.4492725
Produced by FastQC (version 0.11.5)