FastQCFastQC Report
Mon 6 Jun 2016
ERR523127_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523127_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4886615
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT448540.9178951073493615No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173140.3543147966434843No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132190.27051445632610716No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT120330.2462440769326006No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC90360.18491327841460806No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC85820.1756225935540246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84140.17218463087433736No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA68440.14005605107011704No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA62570.12804364575478117No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC57800.11828228743209768No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG182500.030.0112861
TATCACG3650.029.6230472
TACATGG185350.029.4465482
ACATGGG189500.028.1336063
CATGGGG134900.027.9129944
ATGGGGG88150.027.5716655
TATAACG4800.024.484632
TACCTGG33800.024.2006662
TGGGGGG95100.021.3911696
ATAACGC5950.021.3338073
GGGTTAG19500.020.0108261
GAGTACT147050.019.63267912-13
AGAGTAC224250.019.5351510-11
GTACCTG45350.019.3858951
AGTACTT152550.018.53988312-13
TAACGCA6000.018.0203174
GAGTACA135850.017.6494881
GTACTTT165050.017.35032514-15
ACCTGGG45300.017.2278633
AGTACAT130950.016.8368992
Produced by FastQC (version 0.11.5)