##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR523127_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4886615 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29641295661721 34.0 31.0 34.0 30.0 34.0 2 32.6033583574724 34.0 31.0 34.0 30.0 35.0 3 32.4902614591082 34.0 31.0 34.0 30.0 35.0 4 35.09884408736927 37.0 35.0 37.0 33.0 37.0 5 35.108504762499194 37.0 35.0 37.0 33.0 37.0 6 35.27817272283575 37.0 35.0 37.0 33.0 37.0 7 35.154971283802794 37.0 35.0 37.0 33.0 37.0 8 35.29591629379438 37.0 35.0 37.0 33.0 37.0 9 36.60433776755484 39.0 35.0 39.0 33.0 39.0 10-11 36.704868503043514 39.0 35.5 39.0 34.0 39.0 12-13 36.76305274714706 39.0 37.0 39.0 33.0 39.0 14-15 37.911336886576905 40.0 37.5 41.0 33.5 41.0 16-17 37.80085427233371 40.0 36.5 41.0 33.5 41.0 18-19 37.82725045865082 40.0 36.5 41.0 34.0 41.0 20-21 37.851656514785795 40.0 36.0 41.0 34.0 41.0 22-23 37.75673078398851 40.0 36.5 41.0 33.5 41.0 24-25 37.75978484083563 40.0 37.0 41.0 33.0 41.0 26-27 37.57968143592241 39.0 36.5 41.0 33.5 41.0 28-29 37.40011951013125 39.0 37.0 41.0 33.0 41.0 30-31 37.313322616985374 39.0 36.5 41.0 33.0 41.0 32-33 37.13106997379576 39.0 36.0 41.0 32.5 41.0 34-35 37.0266885154652 38.5 35.5 41.0 32.0 41.0 36-37 36.90267229974123 38.0 36.0 41.0 32.0 41.0 38-39 36.65710466242993 38.0 35.5 41.0 31.5 41.0 40-41 36.61914945212585 38.0 35.5 41.0 31.0 41.0 42-43 36.572742788208195 38.0 35.5 40.0 31.0 41.0 44-45 36.47099004116346 38.0 35.5 40.0 31.0 41.0 46-47 36.17677318552822 38.0 35.0 40.0 30.0 41.0 48-49 36.2800281994796 38.0 36.0 40.0 30.5 41.0 50-51 35.24810415799075 37.5 34.0 39.0 28.5 40.0 52-53 35.391835657198286 37.5 35.0 39.5 29.0 40.0 54-55 35.96676063082522 38.0 35.0 40.0 30.0 41.0 56-57 36.10174241269263 38.0 35.0 41.0 30.5 41.0 58-59 36.09525366741599 38.0 35.0 41.0 30.0 41.0 60-61 35.80582642585921 37.5 35.0 40.0 29.5 41.0 62-63 35.599754738198115 37.5 35.0 40.0 29.0 41.0 64-65 35.35667409853242 37.0 35.0 40.0 29.0 41.0 66-67 35.107416585918884 37.0 35.0 39.0 28.5 41.0 68-69 34.756544970291294 37.0 34.5 39.0 28.0 41.0 70-71 34.34798188521093 36.0 34.0 38.5 28.0 40.5 72-73 34.0794061942674 36.0 34.0 38.0 26.5 40.0 74-75 33.816192087979104 36.0 34.0 37.0 26.0 39.0 76-77 33.46945492943479 35.0 34.0 37.0 26.0 39.0 78-79 33.174430152569826 35.0 33.5 37.0 25.5 38.5 80-81 32.94773212131506 35.0 33.0 36.0 25.0 38.5 82-83 32.715212575576345 35.0 33.0 36.0 25.0 38.0 84-85 32.425603817775695 35.0 33.0 36.0 24.5 37.0 86-87 32.33567561594273 35.0 33.0 36.0 24.0 37.0 88-89 32.036715190372064 35.0 32.0 36.0 22.0 37.0 90-91 31.9363989182696 35.0 32.5 35.5 22.5 37.0 92-93 31.693216981489233 35.0 32.0 35.0 20.5 36.0 94-95 31.600157573289486 35.0 32.0 35.0 20.0 36.0 96-97 31.324155882957836 35.0 31.0 35.0 19.0 36.0 98-99 31.280080075880747 35.0 32.0 35.0 18.5 36.5 100 30.04236040694837 34.0 29.0 35.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.020010013121186887 1101 2 0.038994699594908866 1101 3 0.05097488160320296 1101 4 0.026847062964812096 1101 5 0.05302187109634815 1101 6 0.003957640439352872 1101 7 0.07442072034529446 1101 8 0.07684722371828911 1101 9 -0.034900606812819035 1101 10-11 0.009428622105872364 1101 12-13 -0.02851907116530583 1101 14-15 -0.04589497803731035 1101 16-17 -0.10595934811686192 1101 18-19 -0.1008709440544564 1101 20-21 -0.013382173469878467 1101 22-23 -0.10652564450682434 1101 24-25 -0.16632827545720374 1101 26-27 -0.1386578575820394 1101 28-29 -0.08100553257134635 1101 30-31 -0.06091400021270488 1101 32-33 -0.10083584361166231 1101 34-35 -0.22312600878625943 1101 36-37 -0.06668408643734836 1101 38-39 -0.15890592083226096 1101 40-41 -0.1702405419962929 1101 42-43 -0.20431968254762722 1101 44-45 -0.3051571013919201 1101 46-47 -0.16116917459335411 1101 48-49 -0.19187920035460593 1101 50-51 -0.31635576599141757 1101 52-53 -0.25636243470240316 1101 54-55 -0.1752207094549192 1101 56-57 -0.14400572116015553 1101 58-59 -0.08681192108397795 1101 60-61 -0.12363074390236051 1101 62-63 -0.13243153925105844 1101 64-65 -0.031607129949996704 1101 66-67 -0.3336751217440792 1101 68-69 -0.21452830721330685 1101 70-71 -0.1581562034863353 1101 72-73 -0.2105870817005311 1101 74-75 -0.31735906763181987 1101 76-77 -0.2502333468568452 1101 78-79 -0.4898967563925183 1101 80-81 -0.2285174269171648 1101 82-83 -0.2379554283879628 1101 84-85 -0.4153681505718154 1101 86-87 -0.790986915517621 1101 88-89 -0.3221908559231039 1101 90-91 -0.44421698683513 1101 92-93 -0.24813973647240672 1101 94-95 -0.5394383936087443 1101 96-97 -0.5613030042784892 1101 98-99 -0.4547371127032953 1101 100 -0.5508856432326006 1102 1 -0.023463569559616815 1102 2 -0.048582246211950064 1102 3 0.03357768810310091 1102 4 -0.038884391316329925 1102 5 0.045114840179991234 1102 6 0.06154912344980801 1102 7 0.1644314614838578 1102 8 -0.014877785879718886 1102 9 0.037987292798511874 1102 10-11 0.07625705290748641 1102 12-13 0.025437679575574634 1102 14-15 0.03923566267807388 1102 16-17 -0.0014838737165803195 1102 18-19 -0.014895247498095898 1102 20-21 0.016759014267108796 1102 22-23 -0.0578777769894927 1102 24-25 0.03406384080592062 1102 26-27 -0.045532186260878404 1102 28-29 -0.2894198558149128 1102 30-31 -0.10629207534891094 1102 32-33 -0.046150507206967006 1102 34-35 -0.19499199043573157 1102 36-37 -0.16265249282336924 1102 38-39 -0.2726450880273532 1102 40-41 -0.6328415367378781 1102 42-43 -0.05122567385981114 1102 44-45 -0.19330130009866764 1102 46-47 -0.2024462053803049 1102 48-49 -0.45824520141577807 1102 50-51 -0.47337438827863565 1102 52-53 -0.4592539146932566 1102 54-55 -0.19636565443354925 1102 56-57 -0.11517331311767265 1102 58-59 -0.10021763847556286 1102 60-61 -0.5845826072095051 1102 62-63 -0.2325661270113315 1102 64-65 -0.15437359861736155 1102 66-67 -0.19537282555610602 1102 68-69 -0.36070916382988827 1102 70-71 -0.13145002323364707 1102 72-73 -0.42770388403338444 1102 74-75 -0.2879783870146113 1102 76-77 -0.47823881664146484 1102 78-79 -0.3338922873886858 1102 80-81 -0.2943606062941768 1102 82-83 -0.36596305502771287 1102 84-85 -0.40521497072029433 1102 86-87 -0.41626827726967974 1102 88-89 -0.3388698166640296 1102 90-91 -0.2683073331829249 1102 92-93 -0.40026359172154713 1102 94-95 -0.543190458797536 1102 96-97 -0.35909438462263665 1102 98-99 -0.48454873297601253 1102 100 -0.8973818320695806 1103 1 -0.17416200388075964 1103 2 -0.15627950904502086 1103 3 -0.20181229679014479 1103 4 -0.0654967410435745 1103 5 -0.045182697945598704 1103 6 -0.0306803973445966 1103 7 0.03991231927998484 1103 8 -0.16554331983374482 1103 9 -0.15858482839651344 1103 10-11 -0.14486742419535403 1103 12-13 -0.10163541890398164 1103 14-15 -0.19675938868892473 1103 16-17 -0.19225573852374112 1103 18-19 -0.2704912800793764 1103 20-21 -0.24188637317323014 1103 22-23 -0.29432860288662255 1103 24-25 -0.252551819847163 1103 26-27 -0.4392558406717697 1103 28-29 -0.4089468895373969 1103 30-31 -0.33968447267462665 1103 32-33 -0.3941030272771684 1103 34-35 -0.3973817973797793 1103 36-37 -0.44384011513080424 1103 38-39 -0.3069266830528221 1103 40-41 -0.3000618304154372 1103 42-43 -0.2635095481495995 1103 44-45 -0.29840792013559536 1103 46-47 -0.37290614076241724 1103 48-49 -0.4244812649442977 1103 50-51 -0.5013782799220579 1103 52-53 -0.3593572375128389 1103 54-55 -0.29283018359469537 1103 56-57 -0.276036981699221 1103 58-59 -0.23607108770697494 1103 60-61 -0.21917970631103145 1103 62-63 -0.21495437771579162 1103 64-65 -0.2866899972700665 1103 66-67 -0.3175799336281955 1103 68-69 -0.24970404325330975 1103 70-71 -0.24770660379665088 1103 72-73 -0.27686222866969246 1103 74-75 -0.28575519692974183 1103 76-77 -0.43118260962817345 1103 78-79 -0.44564282322730264 1103 80-81 -0.3866798850345816 1103 82-83 -0.4097260266828755 1103 84-85 -0.39997526086497714 1103 86-87 -0.46297589705997666 1103 88-89 -0.3720387395517015 1103 90-91 -0.40929676472333654 1103 92-93 -0.4494091678820453 1103 94-95 -0.51774346096256 1103 96-97 -0.4677454317094565 1103 98-99 -0.4634300264931106 1103 100 -0.5261932318901152 1104 1 0.06841402186556422 1104 2 0.06233838573930228 1104 3 0.10053183876213723 1104 4 0.032466705501583704 1104 5 0.09865027430144835 1104 6 0.048589051629242874 1104 7 0.13475912445908023 1104 8 0.024905780398185584 1104 9 0.0686915001978079 1104 10-11 0.006320565319967386 1104 12-13 0.005862358081813568 1104 14-15 0.019924785191648198 1104 16-17 0.011008039363304079 1104 18-19 -0.05819996539926109 1104 20-21 -0.07871043058617033 1104 22-23 -0.03945003015330428 1104 24-25 -4.045957901013253E-4 1104 26-27 -0.06696728159139553 1104 28-29 -0.23052497729408827 1104 30-31 -0.17938281712638826 1104 32-33 -0.11436117382594801 1104 34-35 -0.18580323174759883 1104 36-37 -0.2741430033298258 1104 38-39 -0.25505274124554944 1104 40-41 -0.014997538298636925 1104 42-43 -0.06533820343926067 1104 44-45 -0.13546318889142128 1104 46-47 -0.22174208968244358 1104 48-49 -0.18982848167690491 1104 50-51 -0.25382870927307977 1104 52-53 -0.20555160688915208 1104 54-55 -0.18571091078719348 1104 56-57 -0.6915590249339942 1104 58-59 -0.07996893043413422 1104 60-61 -0.03563740294227813 1104 62-63 -0.04111788169532815 1104 64-65 -0.09154443059237138 1104 66-67 -0.14093156467973955 1104 68-69 -0.08396068114472399 1104 70-71 -0.11169716776564798 1104 72-73 -0.11824754056383568 1104 74-75 -0.07343282034332077 1104 76-77 -0.10025839398038983 1104 78-79 -0.12227080431058113 1104 80-81 -0.1884809396655669 1104 82-83 -0.0269633525988624 1104 84-85 -0.09730404857527475 1104 86-87 -0.21187749803216605 1104 88-89 -0.14329381474563263 1104 90-91 -0.35194699515310646 1104 92-93 -0.4194081228085622 1104 94-95 -0.1526025792510346 1104 96-97 -0.2028323000931742 1104 98-99 -0.22381964239287555 1104 100 -0.24653210366630063 1105 1 -7.658563514922889E-5 1105 2 -0.04794088739140534 1105 3 -0.007626322628190962 1105 4 -0.02430684450679621 1105 5 -0.09546510990294621 1105 6 -0.11875962606166013 1105 7 0.036904999151111895 1105 8 -0.10031038437347206 1105 9 -0.056308162970296394 1105 10-11 -0.15238526150012177 1105 12-13 -0.10760799072271965 1105 14-15 -0.0666941380706092 1105 16-17 -0.05835017740683668 1105 18-19 -0.12243390049246017 1105 20-21 -0.1520138780257696 1105 22-23 -0.14552547863917198 1105 24-25 -0.15794777183145925 1105 26-27 -0.17593878747155145 1105 28-29 -0.14599215848502922 1105 30-31 -0.06466937279493834 1105 32-33 -0.0474010143023591 1105 34-35 -0.15484972802223496 1105 36-37 -0.10645754020455911 1105 38-39 -0.03273892914013743 1105 40-41 -0.10101269619845965 1105 42-43 -0.12597249738617933 1105 44-45 -0.16631959211218827 1105 46-47 -0.07372777177167222 1105 48-49 -0.08965818411729032 1105 50-51 -0.13090799578414902 1105 52-53 -0.12958930139794944 1105 54-55 -0.1869456203614419 1105 56-57 -0.09698594426019724 1105 58-59 -0.08267183215959051 1105 60-61 -0.03518254050674585 1105 62-63 -0.017776407096398827 1105 64-65 -0.0931984610298926 1105 66-67 -0.1355392809641458 1105 68-69 -0.01261181111119214 1105 70-71 0.024174220989927164 1105 72-73 0.0558102244798917 1105 74-75 0.04181848644828534 1105 76-77 -0.08061997953406319 1105 78-79 -0.03852995858841268 1105 80-81 -0.10881991799171686 1105 82-83 -0.07330408059247873 1105 84-85 -0.042501522661289926 1105 86-87 0.0030412046865109232 1105 88-89 0.05529413949540185 1105 90-91 -0.01917048752839179 1105 92-93 -0.10511286108088669 1105 94-95 -0.06350675684925378 1105 96-97 -0.06938800581638915 1105 98-99 0.028552606561667915 1105 100 -0.03083127728989865 1106 1 0.04978898794414022 1106 2 0.02883475060433227 1106 3 0.08699085015441455 1106 4 0.0934644298840368 1106 5 0.07168624608591756 1106 6 0.10408964314791547 1106 7 0.21086602704739477 1106 8 0.01119655926952845 1106 9 -0.0074300351611711335 1106 10-11 0.006681595679864927 1106 12-13 0.041535811397949374 1106 14-15 0.12028927420642788 1106 16-17 -0.03419391947692674 1106 18-19 0.004752608460755425 1106 20-21 -0.012211582584299663 1106 22-23 -0.07389778938835434 1106 24-25 0.02199626940571875 1106 26-27 0.003785711918744994 1106 28-29 -0.05021394666814416 1106 30-31 -0.05556297225148654 1106 32-33 -0.04557841637343785 1106 34-35 0.019840982632246096 1106 36-37 0.07730559662441294 1106 38-39 0.006823304110717743 1106 40-41 -0.04303898185250432 1106 42-43 0.07736926707793401 1106 44-45 0.0019998079354834886 1106 46-47 -0.05916522329471263 1106 48-49 -0.08626256414897426 1106 50-51 -0.10427793533254004 1106 52-53 -0.05787231997510389 1106 54-55 -0.014721575859923064 1106 56-57 0.03619920799488341 1106 58-59 0.07163522030646874 1106 60-61 0.10103817345586918 1106 62-63 0.09375378447175109 1106 64-65 0.06255429715472616 1106 66-67 0.047439949838626205 1106 68-69 0.13804155190476308 1106 70-71 0.01576153678185932 1106 72-73 0.1060777113415341 1106 74-75 0.19786987758485708 1106 76-77 0.09031069664168712 1106 78-79 0.07960861988545531 1106 80-81 0.17468660289805626 1106 82-83 0.19445525460967872 1106 84-85 0.13554128924662479 1106 86-87 0.10556720339723569 1106 88-89 0.1654077618688916 1106 90-91 0.19155831609923268 1106 92-93 0.06449631721747551 1106 94-95 0.09285602951834093 1106 96-97 0.12359825277724568 1106 98-99 0.09012751645516204 1106 100 0.0684450248697317 1107 1 -0.05313447227624124 1107 2 -0.007747996823702863 1107 3 -0.01332087794793324 1107 4 0.0815882598890525 1107 5 0.05485583352144374 1107 6 0.023218735406956625 1107 7 0.12304115185398246 1107 8 0.032790597603067795 1107 9 -0.08962137649340463 1107 10-11 -0.04761232329389742 1107 12-13 -0.0026734450085612593 1107 14-15 4.0348195054917824E-4 1107 16-17 0.05230812980319399 1107 18-19 -0.08352608167459152 1107 20-21 -0.034762763323378465 1107 22-23 -0.10389434615346715 1107 24-25 -0.03879111639295019 1107 26-27 -0.07341537385368468 1107 28-29 -0.030653723806260302 1107 30-31 -0.05157527049544086 1107 32-33 -0.059824672603078 1107 34-35 0.014247541524810003 1107 36-37 0.06055440836661319 1107 38-39 -0.0020231182144243576 1107 40-41 -0.05127912142740598 1107 42-43 -0.03342266840456176 1107 44-45 0.11740153433757428 1107 46-47 0.06564539878591091 1107 48-49 0.03342443041959342 1107 50-51 -0.08533273146825593 1107 52-53 -0.009228702476633543 1107 54-55 0.09104785043562202 1107 56-57 0.08691820755998947 1107 58-59 0.07900252546679098 1107 60-61 0.09004899956737944 1107 62-63 0.10069617479447146 1107 64-65 0.0584795960454656 1107 66-67 0.10817457639289074 1107 68-69 0.153918560066856 1107 70-71 0.11727442413511113 1107 72-73 0.058440727410250304 1107 74-75 0.08420833048730714 1107 76-77 0.1452618847286118 1107 78-79 0.09383372969731596 1107 80-81 0.09090035174354227 1107 82-83 0.014685690755612768 1107 84-85 0.06962747777355816 1107 86-87 -0.039960670999029446 1107 88-89 -0.021368827722284323 1107 90-91 -0.030551160828110824 1107 92-93 -0.09372829362435908 1107 94-95 0.002541387909538173 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0.14146200077162518 2316 62-63 0.21784331107141952 2316 64-65 0.1746677259919025 2316 66-67 0.15953275380642395 2316 68-69 0.20439305528969243 2316 70-71 0.25124514260792097 2316 72-73 0.28905108723237305 2316 74-75 0.27288756913947765 2316 76-77 -1.133099645370585 2316 78-79 0.23966776589976035 2316 80-81 -0.8212762698926355 2316 82-83 0.21501580494842187 2316 84-85 -1.362820943348126 2316 86-87 0.2565674484512357 2316 88-89 0.38866861177240253 2316 90-91 -0.4320540121338041 2316 92-93 0.39254678123120357 2316 94-95 0.44784264932530604 2316 96-97 0.5191080048265952 2316 98-99 0.4809791799501788 2316 100 0.5152883198621971 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 10759.0 1 894.0 2 612.0 3 492.0 4 435.0 5 470.0 6 526.0 7 804.0 8 1245.0 9 1688.0 10 2347.0 11 3342.0 12 5004.0 13 6154.0 14 6967.0 15 8190.0 16 9195.0 17 11934.0 18 15388.0 19 19675.0 20 24409.0 21 29821.0 22 36100.0 23 39758.0 24 41042.0 25 40483.0 26 42121.0 27 45683.0 28 52856.0 29 63553.0 30 79352.0 31 102412.0 32 136187.0 33 195305.0 34 319219.0 35 598230.0 36 833189.0 37 846706.0 38 982261.0 39 271367.0 40 440.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.40091416029874 17.75645247408061 12.427630343298112 23.41500302232254 2 16.067379983453062 20.94149042155333 39.269314942016024 23.72181465297759 3 20.736621844000528 25.593669117582124 29.42178781218059 24.247921226236755 4 12.924702489805414 15.90620140026783 38.07832718656567 33.09076892336108 5 13.174084369117042 38.02833389950745 34.01070212847237 14.786879602903138 6 30.55448544347691 35.98828311081274 18.329826079478554 15.127405366231805 7 27.249938876673347 32.540888990801015 22.44432131963158 17.764850812894057 8 25.87999554988663 33.3791960484307 20.68424187761942 20.05656652406325 9 26.888354774328615 16.542829915761995 20.901572877965542 35.66724243194385 10-11 25.55568622235576 25.09479842149032 28.41005458451652 20.939460771637396 12-13 26.658813005974093 23.174531889940102 27.78242903688354 22.384226067202267 14-15 23.387464607599313 24.778297579479432 26.97278290653123 24.861454906390033 16-17 22.475493762188474 27.368430125285688 27.221209031174798 22.934867081351044 18-19 22.201297254492573 27.54828219641826 28.381806334668507 21.868614214420663 20-21 23.541618942758404 25.96386899879551 27.87067005598784 22.623842002458243 22-23 22.75635348445425 25.853998496357576 28.252016709601 23.137631309587167 24-25 23.288823900261157 25.646846492179183 28.191785509066314 22.87254409849335 26-27 22.798359466883177 25.203283652206732 29.230310765210987 22.768046115699104 28-29 22.1815789230575 27.513921379235306 28.123280036049316 22.181219661657877 30-31 23.47315360819685 25.740154653575047 28.137453378246484 22.649238359981616 32-33 23.24129623030958 25.9150822100235 28.3646030552276 22.479018504439324 34-35 23.744293877654908 25.99582027404166 28.12623252704491 22.133653321258524 36-37 23.277085239848308 25.793541312306346 28.709005565894703 22.220367881950644 38-39 22.830673723831378 26.628137270852143 28.369607112045408 22.171581893271075 40-41 22.984070180078547 26.52104133358905 27.96025491654391 22.534633569788497 42-43 22.511065693742324 26.845582405906192 27.150137887514823 23.493214012836656 44-45 22.66431353818906 26.16234636615805 27.778930031236047 23.39441006441684 46-47 23.197967834375753 26.370737833105185 27.53537826618939 22.89591606632967 48-49 23.074708162978087 26.45283095179021 27.58449721007307 22.88796367515862 50-51 22.135235173979456 26.743484507685856 28.544997065495792 22.576283252838895 52-53 22.850402877813067 27.34591805033462 26.9561098189827 22.847569252869608 54-55 22.236295852462266 26.21786924165846 27.857594893821837 23.68824001205744 56-57 23.00449228401732 26.21665041512532 27.29643930560109 23.48241799525627 58-59 23.756044342097468 26.35152983028669 27.155012447230547 22.73741338038529 60-61 23.223342737891407 26.454987298355164 28.310179822526987 22.011490141226442 62-63 22.68813688263675 26.618615517054632 27.968140660748652 22.725106939559964 64-65 22.41661862907295 27.24430183453132 27.333895582782848 23.00518395361288 66-67 22.791876948091776 26.310973059170667 26.964818482627372 23.93233151011019 68-69 22.11314732266828 26.580029321692805 27.548439771187727 23.758383584451188 70-71 23.06710284110646 26.286793490487696 27.37177039295026 23.27433327545558 72-73 22.715691682606863 26.246754628338454 27.86828011423544 23.169273574819243 74-75 22.20765743903745 27.622546530786035 27.115268377320817 23.0545276528557 76-77 22.42962332732729 26.24962838620241 27.080801158285304 24.239947128184998 78-79 22.485236988946337 26.40155821260153 27.577743029001606 23.535461769450528 80-81 23.04580977296102 26.320846554197047 27.11502759786314 23.51831607497879 82-83 22.89340809345467 26.149130875597425 27.74634706529969 23.211113965648213 84-85 22.671311423314748 26.906451817922612 27.21070952634756 23.211527232415083 86-87 22.295019486359344 27.198248922903385 27.0549543364016 23.451777254335674 88-89 21.88352219148762 26.801975569944453 26.88755745541318 24.42694478315474 90-91 22.9832087046627 25.850202046711214 26.990752548532075 24.17583670009401 92-93 23.28130184191767 26.47477423614784 26.990247149413126 23.253676772521363 94-95 22.85225629422571 26.20941625448546 27.064174590964146 23.87415286032468 96-97 21.801855089429893 26.20023978044942 28.02067143660323 23.977233693517455 98-99 22.366078208155795 26.607821842372687 26.819351475465652 24.206748474005863 100 22.238224793588053 25.509554781169857 26.942227659262443 25.309992765979644 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6936.0 1 5424.5 2 3366.5 3 2371.5 4 1576.5 5 1398.0 6 1866.0 7 3094.0 8 4849.5 9 5849.0 10 5584.5 11 5037.0 12 5171.0 13 5551.5 14 5941.0 15 6365.5 16 6585.5 17 6858.5 18 7269.5 19 7607.0 20 8150.0 21 9085.0 22 10402.5 23 12312.0 24 14820.0 25 18068.5 26 22362.5 27 27596.0 28 33492.5 29 40067.0 30 47255.0 31 54577.5 32 62307.0 33 70278.5 34 78584.5 35 86360.5 36 95708.0 37 105702.5 38 113412.5 39 120216.5 40 125945.0 41 131032.5 42 135881.5 43 141791.0 44 148548.0 45 156096.5 46 166269.0 47 199249.5 48 264227.5 49 266451.0 50 215665.5 51 195904.5 52 187816.0 53 180838.0 54 172809.0 55 161388.5 56 146599.5 57 132493.5 58 118525.5 59 100937.0 60 83846.0 61 69893.5 62 57273.5 63 45031.5 64 34053.0 65 26074.0 66 20081.0 67 15942.0 68 12738.0 69 9994.0 70 8064.0 71 6401.5 72 5145.5 73 4322.0 74 3499.5 75 2693.5 76 2032.0 77 1403.5 78 971.5 79 701.0 80 518.5 81 379.0 82 255.0 83 178.5 84 142.0 85 107.0 86 74.5 87 63.5 88 58.0 89 44.0 90 33.0 91 29.5 92 31.5 93 23.5 94 13.5 95 11.5 96 10.0 97 7.0 98 6.5 99 4.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19586155242432646 2 0.17130467614084596 3 0.3535780903549799 4 0.35030384018384914 5 0.3617432517192371 6 0.2932909590790353 7 0.3134275976314893 8 0.3034615986731101 9 0.3154944680520156 10-11 0.289996244844335 12-13 0.29830465465357925 14-15 0.28493138911086713 16-17 0.279784677123121 18-19 0.30103660714011643 20-21 0.303748095563084 22-23 0.29562386232596594 24-25 0.29982922738951195 26-27 0.29006786906682847 28-29 0.3173976259639853 30-31 0.30069895009121855 32-33 0.30088312666334466 34-35 0.302141666572873 36-37 0.3102556677782064 38-39 0.3160163016730395 40-41 0.3402969130983308 42-43 0.3121383616266066 44-45 0.2971279709983291 46-47 0.3022132907953665 48-49 0.32309686766810974 50-51 0.3123634663258718 52-53 0.33823004267780454 54-55 0.3395602068098264 56-57 0.34421578127190294 58-59 0.33263312129152794 60-61 0.31505449068526986 62-63 0.3367873261961501 64-65 0.33351307602501934 66-67 0.3205183956583443 68-69 0.3306890352524191 70-71 0.3510917066312775 72-73 0.35552217639408873 74-75 0.34382696406408114 76-77 0.32674970301527745 78-79 0.33600969178050655 80-81 0.3467533251545292 82-83 0.3514498277437449 84-85 0.3457301219760509 86-87 0.34638497201027707 88-89 0.336132476161924 90-91 0.34684541344059233 92-93 0.3282231155922863 94-95 0.34774583223765326 96-97 0.3597070773940652 98-99 0.34961829405426864 100 0.3673913332644376 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4886615.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.02770079417581 #Duplication Level Percentage of deduplicated Percentage of total 1 76.7137946564428 46.049527124227914 2 14.026637163275907 16.839735575711863 3 4.281075847804622 7.7094942020756525 4 1.8033213828756747 4.329969456280015 5 0.910317201575336 2.732212430197785 6 0.5585449036794636 2.0116899814909552 7 0.3602621219224663 1.5137994803565684 8 0.2466447958707771 1.184441600717726 9 0.19222769206349144 1.0385087745188006 >10 0.8494188524424906 8.728184613255866 >50 0.0388324071303121 1.6003514964678007 >100 0.016707080050704974 1.84277847696774 >500 0.0012266561624116748 0.520160213697249 >1k 5.935432221691511E-4 0.7558818527832953 >5k 2.3741728886761672E-4 1.066804564436199 >10k+ 1.5827819257841115E-4 2.076460156814564 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 44854 0.9178951073493615 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17314 0.3543147966434843 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13219 0.27051445632610716 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 12033 0.2462440769326006 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9036 0.18491327841460806 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8582 0.1756225935540246 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8414 0.17218463087433736 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6844 0.14005605107011704 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6257 0.12804364575478117 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5780 0.11828228743209768 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 2.0464063569567073E-5 0.0 6 0.0 0.0 0.0 2.0464063569567073E-5 2.0464063569567073E-5 7 0.0 0.0 0.0 2.0464063569567073E-5 2.0464063569567073E-5 8 0.0 0.0 0.0 2.0464063569567073E-5 2.0464063569567073E-5 9 0.0 0.0 0.0 2.0464063569567073E-5 4.0928127139134146E-5 10-11 0.0 0.0 0.0 2.0464063569567073E-5 4.0928127139134146E-5 12-13 0.0 0.0 0.0 6.139219070870122E-5 4.0928127139134146E-5 14-15 0.0 0.0 0.0 1.0232031784783537E-4 7.162422249348475E-5 16-17 0.0 0.0 0.0 1.0232031784783537E-4 1.432484449869695E-4 18-19 0.0 0.0 0.0 1.125523496326189E-4 1.8417657212610366E-4 20-21 0.0 1.0232031784783537E-5 0.0 1.5348047677175305E-4 2.1487266748045428E-4 22-23 0.0 2.0464063569567073E-5 0.0 2.558007946195884E-4 2.558007946195884E-4 24-25 0.0 2.0464063569567073E-5 0.0 4.502093985304756E-4 2.9672892175872253E-4 26-27 0.0 2.0464063569567073E-5 0.0 9.413469242000854E-4 3.069609535435061E-4 28-29 0.0 2.0464063569567073E-5 0.0 0.0025989360733350182 3.069609535435061E-4 30-31 0.0 2.0464063569567073E-5 0.0 0.0083288738728138 3.1719298532828966E-4 32-33 0.0 2.0464063569567073E-5 0.0 0.02582564822479365 3.2742501711307317E-4 34-35 0.0 2.0464063569567073E-5 0.0 0.05162060035423294 3.683531442522073E-4 36-37 0.0 2.0464063569567073E-5 0.0 0.08945865389436246 3.683531442522073E-4 38-39 0.0 2.0464063569567073E-5 0.0 0.15185358371797247 3.888172078217744E-4 40-41 0.0 2.0464063569567073E-5 0.0 0.22532980396450303 3.888172078217744E-4 42-43 0.0 2.0464063569567073E-5 0.0 0.29807954995431396 4.092812713913414E-4 44-45 0.0 2.0464063569567073E-5 0.0 0.3895846102056332 4.297453349609085E-4 46-47 0.0 2.0464063569567073E-5 0.0 0.4949950016524731 4.297453349609085E-4 48-49 0.0 2.0464063569567073E-5 0.0 0.6077519919207877 5.116015892391769E-4 50-51 0.0 2.0464063569567073E-5 0.0 0.7296871147000531 5.320656528087439E-4 52-53 0.0 2.0464063569567073E-5 0.0 0.8608310660856237 6.139219070870122E-4 54-55 0.0 2.0464063569567073E-5 0.0 1.0126027935493178 6.855461295804969E-4 56-57 0.0 2.0464063569567073E-5 0.0 1.1898829762524774 7.264742567196311E-4 58-59 0.0 2.0464063569567073E-5 0.0 1.389335971833263 7.571703520739817E-4 60-61 0.0 2.0464063569567073E-5 0.0 1.6011697258736364 7.776344156435488E-4 62-63 0.0 2.0464063569567073E-5 0.0 1.8292417143564617 7.980984792131158E-4 64-65 0.0 2.0464063569567073E-5 0.0 2.061079090536087 7.980984792131158E-4 66-67 0.0 2.0464063569567073E-5 0.0 2.3351747579868682 7.980984792131158E-4 68-69 0.0 2.0464063569567073E-5 0.0 2.661320771126843 8.083305109978994E-4 70-71 0.0 2.0464063569567073E-5 0.0 3.006928108721477 8.3902660635225E-4 72-73 0.0 2.0464063569567073E-5 0.0 3.337709232259959 9.618109877696524E-4 74-75 0.0 2.0464063569567073E-5 0.0 3.683940723793464 9.618109877696524E-4 76-77 0.0 2.0464063569567073E-5 0.0 4.063886760057831 9.618109877696524E-4 78-79 0.0 2.0464063569567073E-5 0.0 4.518485700223979 9.618109877696524E-4 80-81 0.0 2.0464063569567073E-5 0.0 4.976717011673725 9.720430195544359E-4 82-83 0.0 2.0464063569567073E-5 0.0 5.440340603874052 0.0010232031784783535 84-85 0.0 2.0464063569567073E-5 0.0 5.92871548096177 0.0010436672420479206 86-87 0.0 2.0464063569567073E-5 0.0 6.436950731743753 0.0010538992738327042 88 0.0 2.0464063569567073E-5 0.0 6.8435716748710504 0.0010641313056174878 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 18250 0.0 30.011286 1 TATCACG 365 0.0 29.623047 2 TACATGG 18535 0.0 29.446548 2 ACATGGG 18950 0.0 28.133606 3 CATGGGG 13490 0.0 27.912994 4 ATGGGGG 8815 0.0 27.571665 5 TATAACG 480 0.0 24.48463 2 TACCTGG 3380 0.0 24.200666 2 TGGGGGG 9510 0.0 21.391169 6 ATAACGC 595 0.0 21.333807 3 GGGTTAG 1950 0.0 20.010826 1 GAGTACT 14705 0.0 19.632679 12-13 AGAGTAC 22425 0.0 19.53515 10-11 GTACCTG 4535 0.0 19.385895 1 AGTACTT 15255 0.0 18.539883 12-13 TAACGCA 600 0.0 18.020317 4 GAGTACA 13585 0.0 17.649488 1 GTACTTT 16505 0.0 17.350325 14-15 ACCTGGG 4530 0.0 17.227863 3 AGTACAT 13095 0.0 16.836899 2 >>END_MODULE