FastQCFastQC Report
Mon 6 Jun 2016
ERR523127_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523127_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4886615
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT453510.9280657469434362No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155410.31803201193464187No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125800.2574379197051538No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT118510.2425196173629394No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC94780.19395839451235672No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC88190.180472576620012No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78530.1607042912118102No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG74100.151638711050492No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA69360.1419387449185172No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC57160.1169725873636454No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG183050.028.8264661
TACATGG185650.028.2039242
ACATGGG190100.026.8720073
CATGGGG135200.026.591174
ATGGGGG86000.025.5194385
TACCTGG40100.023.7941932
GGGTTAG22250.021.7708571
TGGGGGG87100.020.287216
GAGTACT154600.019.64184812-13
TATAACG3850.019.5334342
GTACCTG51150.019.2162631
AGAGTAC235500.019.02222410-11
AGTACTT161200.018.57520912-13
GAGTACG5600.018.4757881
ACCTGGG51700.017.9981273
GTACTTT172500.017.35688814-15
GAGTACA137800.017.2690941
GTTAGAG29200.017.0598683
GGTTAGA28200.017.0008412
AGTACAT136450.016.396562
Produced by FastQC (version 0.11.5)