##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR523127_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4886615
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.46616195464549	34.0	31.0	34.0	31.0	34.0
2	32.72977981690802	34.0	31.0	34.0	31.0	34.0
3	32.81763490678107	34.0	31.0	34.0	30.0	35.0
4	35.619684792028835	37.0	35.0	37.0	33.0	37.0
5	35.78606335878722	37.0	35.0	37.0	34.0	37.0
6	35.941853614414065	37.0	35.0	37.0	34.0	37.0
7	35.90453145991653	37.0	36.0	37.0	35.0	37.0
8	35.9036197858845	37.0	36.0	37.0	35.0	37.0
9	37.230134356809366	39.0	36.0	39.0	35.0	39.0
10-11	37.11183068443084	39.0	36.0	39.0	34.5	39.0
12-13	37.18093844102717	39.0	36.0	39.0	35.0	39.0
14-15	38.29456535863783	40.0	37.0	41.0	34.0	41.0
16-17	38.32482076038321	40.0	37.0	41.0	34.5	41.0
18-19	38.216757305414895	40.0	37.0	41.0	34.5	41.0
20-21	38.21829702155787	40.0	37.0	41.0	34.5	41.0
22-23	38.062277159137764	39.0	36.5	41.0	34.0	41.0
24-25	38.05516814809434	39.0	36.5	41.0	33.5	41.0
26-27	37.83940273584066	39.0	36.5	41.0	34.0	41.0
28-29	37.7461368452395	39.0	37.0	41.0	33.5	41.0
30-31	37.471614297422654	39.0	36.0	41.0	33.5	41.0
32-33	37.357698427234396	38.0	36.5	41.0	33.0	41.0
34-35	37.28468704819184	38.5	36.5	41.0	32.5	41.0
36-37	37.18536430637568	38.0	36.0	41.0	33.0	41.0
38-39	37.16510877161389	38.0	36.0	41.0	33.0	41.0
40-41	37.174136493257606	38.0	36.0	41.0	33.0	41.0
42-43	37.14467530181936	38.0	36.0	41.0	33.0	41.0
44-45	37.1747538940555	39.0	35.5	41.0	32.5	41.0
46-47	36.9611944055343	38.0	35.5	41.0	32.5	41.0
48-49	36.77709979198279	38.0	36.0	41.0	31.5	41.0
50-51	36.67726125344436	38.0	35.5	41.0	31.0	41.0
52-53	36.322297029743496	38.0	35.0	41.0	30.5	41.0
54-55	36.365774569922124	38.0	36.0	40.0	30.0	41.0
56-57	36.263411175220476	37.5	35.5	40.0	30.5	41.0
58-59	35.997722452045025	37.5	35.5	40.0	30.5	41.0
60-61	35.77731978066616	37.0	35.0	40.0	29.5	41.0
62-63	35.56010234078191	36.5	35.0	40.0	29.0	41.0
64-65	35.28117899609443	37.0	34.5	39.0	28.5	41.0
66-67	35.00567130825735	36.5	34.5	39.0	29.0	41.0
68-69	34.8212278847423	37.0	34.0	39.0	28.0	40.5
70-71	34.438747067243895	36.0	34.0	37.5	28.0	40.0
72-73	34.14921637984576	36.0	34.0	37.0	26.5	39.5
74-75	33.89012230347592	35.5	34.0	37.0	26.0	39.0
76-77	32.346760692217416	34.5	31.0	36.5	25.5	37.5
78-79	33.254333828222606	35.0	33.0	36.0	26.0	38.5
80-81	33.29671367603136	35.0	33.5	36.0	26.0	37.5
82-83	33.11775441691232	35.0	33.0	36.0	26.0	37.5
84-85	33.04695417993847	35.0	33.0	36.5	26.0	37.0
86-87	32.727785082311584	35.0	33.0	36.0	26.0	36.5
88-89	32.73950198654897	35.0	33.0	35.5	26.0	37.0
90-91	32.62447553163079	35.0	33.0	36.0	25.0	37.0
92-93	32.40352851616098	35.0	33.0	35.0	25.0	37.0
94-95	32.30934788191826	35.0	33.0	35.0	25.0	36.0
96-97	32.06029807954995	35.0	32.5	35.0	24.0	36.5
98-99	31.99498323481592	35.0	32.0	35.0	24.0	36.0
100	30.896132189665035	34.0	30.0	35.0	20.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.1605722925412465
1101	2	-0.16401415460462232
1101	3	-0.20946949940690018
1101	4	-0.13445247566477292
1101	5	-0.12671283416046464
1101	6	-0.16730860401045078
1101	7	-0.1376570713412235
1101	8	-0.07796761656635454
1101	9	-0.19618156228312955
1101	10-11	-0.21298549968530267
1101	12-13	-0.17104093867374104
1101	14-15	-0.2367061466166831
1101	16-17	-0.17993190604290987
1101	18-19	-0.24131143142388822
1101	20-21	-0.2287948444452823
1101	22-23	-0.27121451407650454
1101	24-25	-0.3272268856759908
1101	26-27	-0.4659338694914794
1101	28-29	-0.3165473143634827
1101	30-31	-0.21523560392616758
1101	32-33	-0.2603238742538423
1101	34-35	-0.2018950124111356
1101	36-37	-0.3239157816263898
1101	38-39	-0.3146237147896116
1101	40-41	-0.17359022426217763
1101	42-43	-0.2716961841535337
1101	44-45	-0.38098507150911587
1101	46-47	-0.32620821119558485
1101	48-49	-0.3528654787498411
1101	50-51	-0.29255466096913807
1101	52-53	-0.3255094746557248
1101	54-55	-0.5081864982852977
1101	56-57	-0.38254969294400354
1101	58-59	-0.532637977439947
1101	60-61	-0.40489444971103694
1101	62-63	-0.37600140919771974
1101	64-65	-0.3307063035069646
1101	66-67	-0.526084943706536
1101	68-69	-0.7250881389176769
1101	70-71	-0.6206663082070207
1101	72-73	-0.49180619064896547
1101	74-75	-0.6417355947050396
1101	76-77	-0.7117557587460084
1101	78-79	-0.3873601113896754
1101	80-81	-0.3401645958806938
1101	82-83	-0.7641302784949175
1101	84-85	-0.4640181155032934
1101	86-87	-0.48155150327431073
1101	88-89	-0.469536870126241
1101	90-91	-0.5805993932274944
1101	92-93	-0.5586255286482995
1101	94-95	-0.9764016268188627
1101	96-97	-0.6719147901517957
1101	98-99	-0.647571347714976
1101	100	-0.549189180192208
1102	1	-0.06105499671993897
1102	2	-0.17195742068721387
1102	3	-0.14587246896504524
1102	4	-0.056618475863203344
1102	5	-0.08571188083050174
1102	6	-0.09494783979354082
1102	7	-0.21034999124856313
1102	8	-0.12393365041172189
1102	9	-0.17551026269163827
1102	10-11	-0.19385462602956238
1102	12-13	-0.08071357120170575
1102	14-15	-0.22052239981422161
1102	16-17	-0.19731055058886682
1102	18-19	-0.2788855733190658
1102	20-21	-0.1890526317707426
1102	22-23	-0.19467721558069684
1102	24-25	-0.18753557478257932
1102	26-27	-0.15769813385290377
1102	28-29	-0.11774825130135724
1102	30-31	-0.12838336144388762
1102	32-33	-0.16947799880791337
1102	34-35	-0.0398767999263967
1102	36-37	-0.13413409850291913
1102	38-39	-0.11707045125211124
1102	40-41	-0.1639607288368623
1102	42-43	-0.12274118046531868
1102	44-45	-0.177368025451635
1102	46-47	-0.2616099686011424
1102	48-49	-0.2604407853467947
1102	50-51	-0.17128504976384562
1102	52-53	-0.10966996193243261
1102	54-55	-0.22294154424788815
1102	56-57	-0.2873279898049219
1102	58-59	-0.2585141998375917
1102	60-61	0.11454787382586318
1102	62-63	-0.2138296286275292
1102	64-65	-0.22407785631325083
1102	66-67	-0.7397883071064228
1102	68-69	-0.31315771043683327
1102	70-71	-0.3683371203492527
1102	72-73	-0.25607486577913363
1102	74-75	-0.30578211375654263
1102	76-77	-0.32753531089660726
1102	78-79	-0.2606966368686088
1102	80-81	-0.3013200663123641
1102	82-83	-0.2462132829078456
1102	84-85	-0.25953142545632346
1102	86-87	-0.28940720103850737
1102	88-89	-0.26498245482809324
1102	90-91	-0.2935068770834306
1102	92-93	-0.3642954694210587
1102	94-95	-1.1277973277752125
1102	96-97	-0.449875613035168
1102	98-99	-0.6135029581524272
1102	100	-0.5969850899321365
1103	1	-0.1835535080436017
1103	2	-0.27893531438220975
1103	3	-0.27218076446622064
1103	4	-0.1986327799505858
1103	5	-0.1825003219393011
1103	6	-0.12199572771400113
1103	7	-0.26261470499121486
1103	8	-0.24391906980458344
1103	9	-0.1627984486267593
1103	10-11	-0.1849260277901834
1103	12-13	-0.20681558662335675
1103	14-15	-0.2587275594534546
1103	16-17	-0.319624780689594
1103	18-19	-0.28116216122199233
1103	20-21	-0.20250039571426015
1103	22-23	-0.3139802787484882
1103	24-25	-0.3350867364854082
1103	26-27	-0.3577498605411904
1103	28-29	-0.32835146517110303
1103	30-31	-0.3726931319781883
1103	32-33	-0.2710627128795693
1103	34-35	-0.3777084098418797
1103	36-37	-0.45386078000870356
1103	38-39	-0.35119018169366
1103	40-41	-0.3798591392921864
1103	42-43	-0.4237863937948205
1103	44-45	-0.3146833868288752
1103	46-47	-0.3779428597378782
1103	48-49	-0.43673583583907316
1103	50-51	-0.42665097561084764
1103	52-53	-0.2924247775604769
1103	54-55	-0.3824814300026702
1103	56-57	-0.44591520104055604
1103	58-59	-0.4235207035351607
1103	60-61	-0.5435893073881815
1103	62-63	-0.4238205953878449
1103	64-65	-0.3475843983293032
1103	66-67	-0.22159544854889646
1103	68-69	-0.404592334861519
1103	70-71	-0.5277228182724443
1103	72-73	-0.5253132575308612
1103	74-75	-0.5667814441296244
1103	76-77	-0.6239961452943099
1103	78-79	-0.472138810099743
1103	80-81	-0.44594994958525547
1103	82-83	-0.3139672153177031
1103	84-85	-0.3825156741444573
1103	86-87	-0.38984444952858865
1103	88-89	-0.4453100850394591
1103	90-91	-0.42096975352336585
1103	92-93	-0.361303559647439
1103	94-95	-0.9396427393947064
1103	96-97	-0.35165698513453236
1103	98-99	-0.5451015935183108
1103	100	-0.4389216734300696
1104	1	-0.08427572527865124
1104	2	-0.18023723477172382
1104	3	-0.19551953307489356
1104	4	-0.1574958293231674
1104	5	-0.15542872056069257
1104	6	-0.11400413117667796
1104	7	-0.1469219440901881
1104	8	-0.0634370229611747
1104	9	-0.13798090103907157
1104	10-11	-0.16195332519889405
1104	12-13	-0.1509993953981379
1104	14-15	-0.1839231199455682
1104	16-17	-0.24113659113649533
1104	18-19	-0.3206659612252949
1104	20-21	-0.16124694619019664
1104	22-23	-0.9664491531998749
1104	24-25	-0.25936549063276715
1104	26-27	-0.17196009091737352
1104	28-29	-0.17896975565419382
1104	30-31	-0.11289061067140693
1104	32-33	-0.17642626223494773
1104	34-35	-0.14624473083485867
1104	36-37	-0.27693716371502575
1104	38-39	-0.22696274333400623
1104	40-41	-0.20092945085360014
1104	42-43	-0.21860443712250088
1104	44-45	-0.28388366444326607
1104	46-47	-0.24673893061886787
1104	48-49	-0.21223799588090486
1104	50-51	-0.2853240806447559
1104	52-53	-0.24489711325951902
1104	54-55	-0.13178857754604678
1104	56-57	-0.49320508879340963
1104	58-59	-0.27298528201995964
1104	60-61	-0.2758719399193623
1104	62-63	-0.2950134574466361
1104	64-65	-0.3562690482989126
1104	66-67	-0.2940610895250657
1104	68-69	-0.4065341617839451
1104	70-71	-0.21479840790156146
1104	72-73	-0.36676058158447944
1104	74-75	-0.7602788539998571
1104	76-77	-0.44189222479492685
1104	78-79	-0.2537518810213797
1104	80-81	-0.20110564543671217
1104	82-83	-0.11395028870016688
1104	84-85	-0.1278801256572848
1104	86-87	-0.1493533677122869
1104	88-89	-0.08808525534400502
1104	90-91	-0.13744681081080756
1104	92-93	-0.17919550798151107
1104	94-95	-0.16949048469332695
1104	96-97	-0.40996288528892677
1104	98-99	-0.22783304938159787
1104	100	-0.44409867270253756
1105	1	-0.04411709371746042
1105	2	-0.18004405587310401
1105	3	-0.14403712429478333
1105	4	-0.09478079700356545
1105	5	-0.11562160211805406
1105	6	-0.119505223208904
1105	7	-0.16157389917864862
1105	8	-0.10998877032888998
1105	9	-0.07761356379043605
1105	10-11	-0.17122598202200834
1105	12-13	-0.13144398097643517
1105	14-15	-0.956715744395666
1105	16-17	-0.22436849548181215
1105	18-19	-0.3281797051280577
1105	20-21	-0.2798361873613686
1105	22-23	-0.22399553495167623
1105	24-25	-0.20205268437707247
1105	26-27	-0.21099737425896592
1105	28-29	-0.15301839139210216
1105	30-31	-0.242975761774197
1105	32-33	-0.2703533922553092
1105	34-35	-0.16111620739902577
1105	36-37	-0.29660800694230716
1105	38-39	-0.2369536883635064
1105	40-41	-0.2087132642459366
1105	42-43	-0.3052605512718074
1105	44-45	-0.254745452328379
1105	46-47	-0.26802038781404036
1105	48-49	-0.22443254162012494
1105	50-51	-0.28503538086697233
1105	52-53	-0.2723680539589779
1105	54-55	-0.3076901836069794
1105	56-57	-0.35861547315808906
1105	58-59	-0.39275308637312634
1105	60-61	-0.32544899520950565
1105	62-63	-0.2863555769044126
1105	64-65	-0.41825624497017344
1105	66-67	-0.306172612009874
1105	68-69	-0.2614793418124677
1105	70-71	-0.17407633788605636
1105	72-73	-0.22133401535101171
1105	74-75	-0.36542954794324345
1105	76-77	-0.4427235723687346
1105	78-79	-0.29378820776039305
1105	80-81	-0.1742611888301866
1105	82-83	-0.2268470940935714
1105	84-85	-0.28809581886429214
1105	86-87	-0.3086882571315357
1105	88-89	-0.31923291361899686
1105	90-91	-0.1660784045274255
1105	92-93	-0.2611786022726079
1105	94-95	-0.27174702608486
1105	96-97	-0.12964961768969374
1105	98-99	-0.22471091971200607
1105	100	-0.304128574082803
1106	1	0.0646328473965525
1106	2	-0.05399393702411004
1106	3	-0.06879681255017545
1106	4	-0.026859685138049372
1106	5	-0.08194534072767112
1106	6	-0.006010647637211264
1106	7	-0.014719295292643153
1106	8	-0.08548869703882644
1106	9	-0.08658901415659415
1106	10-11	-0.04986758780479761
1106	12-13	-0.05739866074436151
1106	14-15	-0.06597230201803228
1106	16-17	-0.15252478878709042
1106	18-19	-0.22373680334568036
1106	20-21	-0.09006179818637605
1106	22-23	-0.08699533688460548
1106	24-25	-0.17899087396701674
1106	26-27	-0.08287050643485117
1106	28-29	-0.09205659627050267
1106	30-31	-0.08605903120128033
1106	32-33	-0.058868045978563543
1106	34-35	-0.07775050501485481
1106	36-37	-0.12923934195813302
1106	38-39	-0.051490016158027174
1106	40-41	-0.09689803385155926
1106	42-43	-0.1843560826440651
1106	44-45	-0.06642880875786261
1106	46-47	-0.13943957250973682
1106	48-49	-0.08957334173752685
1106	50-51	-0.051505397523165186
1106	52-53	-0.014801051337180127
1106	54-55	-0.15202485688475065
1106	56-57	-0.031240226953364925
1106	58-59	-0.122368355540317
1106	60-61	-0.10068632544054168
1106	62-63	-0.10910991489719635
1106	64-65	-0.10843598426767898
1106	66-67	-0.06327174466524355
1106	68-69	0.3353415022334474
1106	70-71	-0.08172559700839344
1106	72-73	-0.06716542157291627
1106	74-75	-0.112405128602866
1106	76-77	-0.11669716552776777
1106	78-79	-0.02535113643505582
1106	80-81	0.017911458055110074
1106	82-83	0.0954629283277626
1106	84-85	0.10089610469267996
1106	86-87	0.09089686153445342
1106	88-89	0.09972773075219976
1106	90-91	0.06705338695330454
1106	92-93	0.015085029724033916
1106	94-95	0.0858160230762266
1106	96-97	0.12481096302333583
1106	98-99	0.09804066005236578
1106	100	0.17823093533431233
1107	1	-0.04994664647354341
1107	2	-0.13075052082244554
1107	3	-0.1087158239060102
1107	4	-0.0663002320912156
1107	5	-0.1069778720350456
1107	6	-0.053273404053548745
1107	7	-0.07267419318748836
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2316	88-89	0.2119388603594885
2316	90-91	0.243121403800302
2316	92-93	0.21183345031737133
2316	94-95	0.3179881151529784
2316	96-97	0.40056485547090404
2316	98-99	0.2855875625330988
2316	100	0.10784177002124906
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	0.0
11	0.0
12	17.0
13	89.0
14	323.0
15	927.0
16	2205.0
17	4445.0
18	7826.0
19	12035.0
20	16781.0
21	23022.0
22	29902.0
23	34954.0
24	36935.0
25	38820.0
26	43002.0
27	49417.0
28	58833.0
29	72471.0
30	89591.0
31	113092.0
32	144118.0
33	194377.0
34	302823.0
35	604189.0
36	959883.0
37	855078.0
38	975678.0
39	215662.0
40	118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.46231158659993	18.302237904398243	12.86653741976331	23.368913089238514
2	16.14437793331381	21.1858452252083	38.99738819815964	23.67238864331825
3	20.732920436744045	25.559287973372157	29.53803399694881	24.16975759293499
4	13.079362727603131	16.17750342980443	37.820981398836324	32.92215244375612
5	13.262212497695232	38.13193309967456	33.924131090389196	14.681723312241015
6	30.600422052488067	36.24506711829667	18.089054220975093	15.065456608240174
7	27.323699534340236	32.61073360598287	22.203017835454606	17.86254902422229
8	25.932902672879298	33.82216644159552	20.298149859933375	19.946781025591807
9	26.66569394091448	16.721483105268025	20.775068697903578	35.837754255913914
10-11	25.552281872155813	25.313036382565635	28.21149620519327	20.923185540085285
12-13	26.63813155598721	23.158440158379687	27.72590986617209	22.477518419461013
14-15	23.301193133167555	24.87839587656845	26.855724821568316	24.96468616869568
16-17	22.464041121821225	27.49158200236813	27.170414979554096	22.873961896256546
18-19	22.143620890943936	27.66824274062925	28.31782327848623	21.87031308994058
20-21	23.483185535736997	26.016786991069317	27.867641212982942	22.632386260210744
22-23	22.74294042645629	25.904972062734753	28.222782282922786	23.129305227886164
24-25	23.24684500503684	25.695879308236623	28.197967971947186	22.859307714779348
26-27	22.80423976691965	25.26243232999305	29.20708311469089	22.72624478839641
28-29	22.216387264037223	27.554173357198902	28.097718131134297	22.131721247629578
30-31	23.487933542003724	25.820612567559586	28.07265855417798	22.618795336258703
32-33	23.246600315655737	25.97384829860247	28.33033790553904	22.449213480202754
34-35	23.794813989627816	26.02200029489805	28.092405618043177	22.090780097430958
36-37	23.27739073614493	25.832441931305798	28.6915759899167	22.198591342632565
38-39	22.85450176087215	26.658656842045808	28.359363216896188	22.127478180185854
40-41	23.030246473591358	26.527278185739572	27.97144451627519	22.471030824393885
42-43	22.57026902664031	26.899062288352855	27.11831837092362	23.412350314083213
44-45	22.66955705921181	26.211157664221478	27.76776215695646	23.351523119610253
46-47	23.201367365617013	26.416391605391855	27.487350046580318	22.894890982410814
48-49	23.09959880817251	26.47263311762604	27.543255732466736	22.884512341734716
50-51	22.19139800769533	26.809609882378034	28.40780486986204	22.5911872400646
52-53	22.890439784798357	27.374755638728438	26.86299887356412	22.871805702909086
54-55	22.29417353953754	26.266468132601712	27.723722767479188	23.715635560381568
56-57	23.116451023823018	26.252552049636208	27.16608682243485	23.464910104105925
58-59	23.793631610554357	26.33018414325443	27.13754205596671	22.738642190224507
60-61	23.273760604562376	26.522231517528837	28.169325515452947	22.034682362455836
62-63	22.749881051656835	26.679586417889833	27.838649770033204	22.73188276042013
64-65	22.46342222832052	27.2753587687657	27.19381959901228	23.0673994039015
66-67	22.79251588267134	26.37930346466828	26.851521963567826	23.976658689092552
68-69	22.09300731284808	26.66538424939196	27.39500123296235	23.846607204797603
70-71	23.007319725276755	26.381594692659434	27.258473980989407	23.35261160107441
72-73	22.669808143890187	26.335282158401956	27.69239254235464	23.302517155353218
74-75	22.160331620384298	27.74654084575068	26.88377323583645	23.209354298028572
76-77	22.37903304201189	26.367097765103026	26.89100566782397	24.362863525061115
78-79	22.359377807501623	26.557169584046697	27.42604976770514	23.657402840746535
80-81	22.91298502623052	26.4674966338922	26.959671271013043	23.659847068864238
82-83	22.79110967587198	26.300402371746305	27.545080083478528	23.36340786890318
84-85	22.489349203787945	27.09837593839406	27.028265934652197	23.3840089231658
86-87	22.121807284698754	27.346438867415824	26.849929271044104	23.68182457684132
88-89	21.648364601450453	26.980448806298522	26.698279603823266	24.67290698842776
90-91	22.643987133407556	26.126668424532067	26.773221141001986	24.45612330105839
92-93	22.885953066450348	26.74327872629927	26.7764305364188	23.59433767083158
94-95	22.39299399286601	26.50694300840471	26.845552225250795	24.254510773478486
96-97	21.338292420083178	26.5001479581166	27.79908924923637	24.36247037256386
98-99	21.88315312094486	26.877076235191748	26.608123647179227	24.63164699668416
100	21.566360353741803	25.991366211579997	26.73529631452447	25.706977120153724
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	760.0
1	599.5
2	944.5
3	1365.0
4	1051.5
5	982.0
6	1399.5
7	2498.0
8	3994.5
9	4825.5
10	4568.5
11	4075.5
12	4260.0
13	4668.5
14	5110.0
15	5654.5
16	6051.0
17	6424.5
18	6957.5
19	7447.5
20	7991.0
21	8837.0
22	10203.0
23	12300.0
24	14830.5
25	18117.5
26	22718.0
27	28071.5
28	33896.0
29	40612.0
30	48008.0
31	55137.5
32	62912.5
33	70962.5
34	78936.0
35	86622.5
36	96408.0
37	106317.0
38	113529.5
39	120841.5
40	126909.5
41	132195.5
42	137156.5
43	142731.0
44	149351.5
45	157035.0
46	166899.5
47	201318.0
48	268138.0
49	269311.5
50	217074.5
51	196846.0
52	188642.5
53	182514.0
54	174681.0
55	161690.5
56	146335.0
57	132014.5
58	118002.5
59	100995.5
60	83697.0
61	69174.5
62	56663.0
63	44550.5
64	33732.5
65	25956.5
66	19770.5
67	15621.5
68	12518.5
69	9680.5
70	7735.0
71	6169.5
72	4963.0
73	4133.5
74	3286.5
75	2539.5
76	1888.0
77	1257.0
78	862.0
79	640.0
80	469.0
81	314.0
82	212.5
83	147.5
84	92.0
85	65.5
86	48.0
87	25.5
88	13.0
89	11.0
90	10.0
91	4.5
92	3.5
93	4.0
94	3.0
95	1.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.060143882830957625
2	0.014652269515810026
3	0.0
4	7.980984792131158E-4
5	4.092812713913414E-5
6	3.8881720782177434E-4
7	0.0
8	2.4556876283480486E-4
9	6.139219070870122E-5
10-11	5.1160158923917684E-5
12-13	7.162422249348475E-5
14-15	0.015992665679616668
16-17	0.0012483078777435915
18-19	0.0
20-21	6.855461295804969E-4
22-23	8.492586381370335E-4
24-25	0.002404527469424131
26-27	0.00824701761853553
28-29	0.007305670694335445
30-31	0.005740169831263564
32-33	0.005709473735909213
34-35	0.003693763474306857
36-37	0.0024147595012089145
38-39	0.0023738313740697807
40-41	0.002619400136904585
42-43	0.001872461816615387
44-45	0.0016678211809197164
46-47	0.008820011398483409
48-49	0.013772314782318638
50-51	0.013618834305546886
52-53	0.008328873872813797
54-55	0.002701256391182854
56-57	0.002077102452311058
58-59	0.002465919660132832
60-61	7.980984792131158E-4
62-63	8.18562542782683E-4
64-65	1.3301641320218597E-4
66-67	0.0
68-69	2.0464063569567074E-4
70-71	5.013695574543933E-4
72-73	0.003407266584332918
74-75	0.006241539388717958
76-77	0.005320656528087438
78-79	0.0031310017261437623
80-81	7.469383202891981E-4
82-83	0.0018213016576914692
84-85	1.7394454034132012E-4
86-87	5.1160158923917684E-5
88-89	1.6371250855653656E-4
90-91	1.3301641320218597E-4
92-93	8.185625427826828E-5
94-95	1.7394454034132012E-4
96-97	0.001985014166248006
98-99	0.001350628195591427
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4886615.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.53947927066554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.25824582605019	45.403762465774314
2	14.219301906936138	16.93219662262718
3	4.43742020917929	7.92605065678988
4	1.8271894655074414	4.35159637220635
5	0.9709816956201218	2.890587226928496
6	0.5606421342103866	2.00282044368486
7	0.3643704369189431	1.5186098253045133
8	0.24223428052510027	1.1538002339175033
9	0.1786764654833924	0.9574473341523806
>10	0.8803912434903232	9.0151946173171
>50	0.04125061399246838	1.6987293073084064
>100	0.017173303966743877	1.874759097095051
>500	0.001121277563183704	0.45871465677482803
>1k	6.006843338138367E-4	0.6795480593400466
>5k	2.4027373352550314E-4	1.101830634652118
>10k+	1.6018248901700208E-4	2.0343524461270373
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	45351	0.9280657469434362	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15541	0.31803201193464187	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12580	0.2574379197051538	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	11851	0.2425196173629394	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	9478	0.19395839451235672	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	8819	0.180472576620012	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7853	0.1607042912118102	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	7410	0.151638711050492	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	6936	0.1419387449185172	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	5716	0.1169725873636454	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0464063569567073E-5
2	0.0	0.0	0.0	0.0	2.0464063569567073E-5
3	0.0	0.0	0.0	0.0	2.0464063569567073E-5
4	0.0	0.0	0.0	0.0	2.0464063569567073E-5
5	0.0	0.0	0.0	0.0	2.0464063569567073E-5
6	0.0	0.0	0.0	0.0	2.0464063569567073E-5
7	0.0	0.0	0.0	0.0	2.0464063569567073E-5
8	0.0	0.0	0.0	0.0	4.0928127139134146E-5
9	0.0	0.0	0.0	0.0	8.185625427826829E-5
10-11	2.0464063569567073E-5	0.0	0.0	2.0464063569567073E-5	8.185625427826829E-5
12-13	2.0464063569567073E-5	0.0	0.0	2.0464063569567073E-5	8.185625427826829E-5
14-15	2.0464063569567073E-5	0.0	0.0	6.139219070870122E-5	1.5348047677175305E-4
16-17	2.0464063569567073E-5	0.0	0.0	6.139219070870122E-5	1.6371250855653658E-4
18-19	2.0464063569567073E-5	0.0	0.0	7.162422249348475E-5	2.0464063569567074E-4
20-21	2.0464063569567073E-5	0.0	0.0	1.3301641320218597E-4	2.0464063569567074E-4
22-23	2.0464063569567073E-5	0.0	0.0	2.4556876283480486E-4	2.0464063569567074E-4
24-25	2.0464063569567073E-5	0.0	0.0	4.2974533496090855E-4	2.0464063569567074E-4
26-27	2.0464063569567073E-5	0.0	0.0	9.208828606305183E-4	2.251046992652378E-4
28-29	2.0464063569567073E-5	0.0	0.0	0.0025273118508415335	2.251046992652378E-4
30-31	2.0464063569567073E-5	0.0	0.0	0.008206089491396396	2.251046992652378E-4
32-33	2.0464063569567073E-5	0.0	0.0	0.02563123962088276	2.3533673105002132E-4
34-35	2.0464063569567073E-5	0.0	0.0	0.05144665581389162	2.6603282640437194E-4
36-37	2.0464063569567073E-5	0.0	0.0	0.08944842186257768	2.6603282640437194E-4
38-39	2.0464063569567073E-5	0.0	0.0	0.15181265559083335	3.4788908068264025E-4
40-41	2.0464063569567073E-5	0.0	0.0	0.22518655551951605	3.683531442522073E-4
42-43	2.0464063569567073E-5	0.0	0.0	0.2978749093186183	3.683531442522073E-4
44-45	2.0464063569567073E-5	0.0	0.0	0.3892981133156592	3.683531442522073E-4
46-47	2.0464063569567073E-5	0.0	0.0	0.4945550242857275	3.7858517603699083E-4
48-49	2.0464063569567073E-5	0.0	0.0	0.6071380700137007	3.888172078217744E-4
50-51	2.0464063569567073E-5	0.0	0.0	0.7284285747905248	3.888172078217744E-4
52-53	2.0464063569567073E-5	0.0	0.0	0.8588153558240212	4.2974533496090855E-4
54-55	2.0464063569567073E-5	0.0	0.0	1.010372210620235	4.8090549388482616E-4
56-57	2.0464063569567073E-5	0.0	0.0	1.1869463831302445	4.911375256696097E-4
58-59	2.0464063569567073E-5	0.0	0.0	1.3857547607085885	5.116015892391769E-4
60-61	2.0464063569567073E-5	0.0	0.0	1.5963402068712185	5.320656528087439E-4
62-63	2.0464063569567073E-5	0.0	0.0	1.8238187375105261	5.320656528087439E-4
64-65	2.0464063569567073E-5	0.0	0.0	2.0548375511473687	5.422976845935274E-4
66-67	2.0464063569567073E-5	0.0	0.0	2.3288206662485176	5.52529716378311E-4
68-69	2.0464063569567073E-5	0.0	0.0	2.6539946363689384	5.52529716378311E-4
70-71	2.0464063569567073E-5	0.0	0.0	2.9984969145308153	5.72993779947878E-4
72-73	2.0464063569567073E-5	2.0464063569567073E-5	0.0	3.3279478739372754	7.367062885044145E-4
74-75	2.0464063569567073E-5	2.0464063569567073E-5	0.0	3.6735654435636937	7.674023838587652E-4
76-77	2.0464063569567073E-5	2.0464063569567073E-5	0.0	4.052774773539556	7.776344156435488E-4
78-79	2.0464063569567073E-5	2.0464063569567073E-5	0.0	4.5075169621506905	7.776344156435488E-4
80-81	2.0464063569567073E-5	2.0464063569567073E-5	0.0	4.96515481575692	7.980984792131159E-4
82-83	2.0464063569567073E-5	2.0464063569567073E-5	0.0	5.4287886399890315	8.18562542782683E-4
84-85	2.0464063569567073E-5	2.0464063569567073E-5	0.0	5.917961615555963	8.3902660635225E-4
86-87	2.0464063569567073E-5	2.0464063569567073E-5	0.0	6.425654568653352	8.492586381370335E-4
88	2.0464063569567073E-5	2.0464063569567073E-5	0.0	6.832643864924902	8.799547334913841E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	18305	0.0	28.826466	1
TACATGG	18565	0.0	28.203924	2
ACATGGG	19010	0.0	26.872007	3
CATGGGG	13520	0.0	26.59117	4
ATGGGGG	8600	0.0	25.519438	5
TACCTGG	4010	0.0	23.794193	2
GGGTTAG	2225	0.0	21.770857	1
TGGGGGG	8710	0.0	20.28721	6
GAGTACT	15460	0.0	19.641848	12-13
TATAACG	385	0.0	19.533434	2
GTACCTG	5115	0.0	19.216263	1
AGAGTAC	23550	0.0	19.022224	10-11
AGTACTT	16120	0.0	18.575209	12-13
GAGTACG	560	0.0	18.475788	1
ACCTGGG	5170	0.0	17.998127	3
GTACTTT	17250	0.0	17.356888	14-15
GAGTACA	13780	0.0	17.269094	1
GTTAGAG	2920	0.0	17.059868	3
GGTTAGA	2820	0.0	17.000841	2
AGTACAT	13645	0.0	16.39656	2
>>END_MODULE