FastQCFastQC Report
Mon 6 Jun 2016
ERR523126_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523126_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11039856
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT352420.3192251782994271No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323160.29272120940707924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260600.2360538036003368No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161500.14628813999023174No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG135450.039.2654342
GTACCTG154150.035.180931
ACCTGGG168000.031.070513
GTACATG476550.022.1283321
CATGGGG265350.022.0265884
TACATGG481050.021.3495712
CCTGGGG166000.021.2729174
ACATGGG483400.020.7208523
GAGTACT304450.019.81047212-13
ATGGGGG150400.018.9135535
GTACACG23250.017.8025111
AGTACTT321400.017.6033612-13
GTACTTT344450.017.48384514-15
AGAGTAC576450.015.52761910-11
TATCACG10600.015.5270512
GTATTAG30250.015.3932881
GTATCAA825950.015.312941
GTGTAGC64750.015.1093261
GTATAGG30150.014.8203291
ACTTTTT422800.014.59403116-17
Produced by FastQC (version 0.11.5)