FastQCFastQC Report
Mon 6 Jun 2016
ERR523126_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523126_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11039856
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT358590.32481401931329534No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279990.25361743848832813No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244500.22147028004713107No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148080.13413218433283913No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG135650.037.9373362
GTACCTG152650.034.1282651
ACCTGGG162950.031.0877783
CCTGGGG160500.021.3148174
GTACATG468200.021.2197041
TACATGG473250.020.6956752
CATGGGG257550.020.6177944
ACATGGG474300.019.845093
GAGTACT303300.019.00839212-13
TATCACG8700.017.8265462
GTACTTT336350.017.01985714-15
AGTACTT320200.016.9848712-13
ATGGGGG137700.016.7558695
GTATAGG31550.015.4990541
GTATAGC38000.015.4666991
AGAGTAC573750.015.17258610-11
GTATCAA807750.014.5989561
GTGTAGC57850.014.5485911
GTACACG21800.014.4507221
CTGGGGG100150.014.0763395
Produced by FastQC (version 0.11.5)