FastQCFastQC Report
Mon 6 Jun 2016
ERR523125_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523125_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3816493
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193470.5069313634271044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160890.42156503365786335No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105670.27687722733934006No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88390.23160005795896912No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT54800.1435873195627504No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA45120.11822372004874633No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44000.11528908870001857No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG85950.061.2311132
ACCTGGG94600.054.9355353
GTACCTG100550.052.7688751
CCTGGGG85550.041.720254
TATAACG4950.038.9553072
GTACCCG8900.035.410791
CTGGGGG54500.035.2014245
ATAACGC6750.030.6571063
TAACGCA7000.028.2147644
TGGGGGG86800.027.825146
TACCCGG10650.026.9381792
TATCACG4300.025.1563362
CATGGGG107500.025.0214614
GTATAAC10800.024.3901861
ATGGGGG71700.024.0681935
GTATAGG8650.023.3831581
GTACATG195700.022.521621
GGTATCA202250.022.071331
GTATCAA261450.022.0393241
TACATGG199700.021.9024582
Produced by FastQC (version 0.11.5)