##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR523125_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3816493
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.462958794893638	33.0	31.0	34.0	28.0	34.0
2	31.783392763985155	34.0	31.0	34.0	28.0	35.0
3	31.780379002398274	34.0	31.0	34.0	27.0	36.0
4	34.08920126409245	35.0	34.0	37.0	32.0	37.0
5	34.183200126398766	35.0	34.0	37.0	31.0	37.0
6	34.41425727755822	35.0	35.0	37.0	30.0	37.0
7	34.31030032021545	35.0	34.0	37.0	31.0	37.0
8	34.424611547826764	36.0	34.0	37.0	30.0	37.0
9	35.601094774705466	37.0	35.0	39.0	31.0	39.0
10-11	35.562268815899834	37.0	34.5	39.0	30.0	39.0
12-13	35.58694159795394	37.5	35.0	39.0	30.5	39.0
14-15	36.35545394685644	38.0	35.0	41.0	31.0	41.0
16-17	36.5094812174423	38.0	36.0	41.0	31.0	41.0
18-19	36.381223809397795	38.0	35.5	41.0	29.5	41.0
20-21	36.4837367709046	38.0	35.5	41.0	30.0	41.0
22-23	36.32599588155932	38.0	35.5	40.5	30.0	41.0
24-25	36.3014000025678	38.0	35.0	40.5	30.0	41.0
26-27	36.17392407113023	38.0	36.0	40.0	29.5	41.0
28-29	35.938894686823744	38.0	35.0	40.0	29.0	41.0
30-31	35.73090583423054	37.0	35.5	40.0	29.0	41.0
32-33	35.666992707703116	37.5	34.5	40.0	27.5	41.0
34-35	35.49046664044714	37.5	34.5	40.0	27.5	41.0
36-37	35.29576760654349	37.5	34.5	40.0	27.5	41.0
38-39	35.11738014454632	36.5	35.0	40.0	27.0	41.0
40-41	35.057398899984875	37.0	34.0	40.0	26.0	41.0
42-43	34.8307533906128	36.0	35.0	40.0	25.5	41.0
44-45	34.73781191266433	37.0	33.5	40.0	25.0	41.0
46-47	34.47448076021625	36.5	33.5	40.0	23.5	41.0
48-49	34.30161512152649	37.0	33.0	40.0	23.0	41.0
50-51	33.23217873057805	36.5	31.5	38.5	21.5	40.0
52-53	33.44082564804914	36.5	32.5	38.5	22.0	39.5
54-55	34.283202667999134	37.0	33.0	39.5	23.0	41.0
56-57	34.41107975306126	37.0	34.0	40.0	23.5	41.0
58-59	34.33868396981208	37.0	33.0	40.0	23.0	41.0
60-61	34.07543692599462	36.5	33.5	39.0	23.0	41.0
62-63	33.89065196765722	36.0	33.0	39.0	22.5	41.0
64-65	33.48337570644044	36.0	33.0	39.0	22.0	41.0
66-67	33.27781840553618	36.0	33.0	38.0	21.5	40.5
68-69	32.9370568739416	35.0	32.5	37.5	20.0	40.0
70-71	32.72316259980039	35.0	32.0	37.0	20.0	39.5
72-73	32.39425173844155	35.0	32.0	37.0	20.0	39.0
74-75	32.017595211100875	35.0	32.0	36.5	19.5	39.0
76-77	31.902357609459784	35.0	31.0	37.0	18.5	38.0
78-79	31.637429703133215	35.0	31.0	36.0	18.0	38.0
80-81	31.380259180352226	35.0	30.0	36.0	18.0	37.5
82-83	31.076617984102157	34.5	30.0	35.5	17.5	37.0
84-85	30.84853961477199	35.0	30.0	35.0	17.5	37.0
86-87	30.613195674667814	34.0	29.5	35.0	17.0	37.0
88-89	30.460452305297036	34.0	29.0	35.0	17.0	36.5
90-91	30.254385636237245	34.0	29.0	35.0	17.0	36.0
92-93	30.002131407027342	34.0	29.0	35.0	15.5	36.0
94-95	29.901670591299393	34.0	29.0	35.0	15.0	36.0
96-97	29.712642470456515	34.0	29.0	35.0	14.0	36.0
98-99	29.62376270570914	34.0	28.0	35.0	12.0	36.0
100	28.391022595875324	33.0	25.0	35.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.03946280601527974
1101	2	0.010727974817804409
1101	3	0.03067280360181357
1101	4	-0.034928325545628525
1101	5	0.006549615956011223
1101	6	0.08052940886540227
1101	7	0.061833126378367353
1101	8	0.13888707064413808
1101	9	0.17471306229789008
1101	10-11	0.10279186562067366
1101	12-13	0.04416650859160853
1101	14-15	-0.14110602595334143
1101	16-17	0.014214240633023678
1101	18-19	0.007630971678835863
1101	20-21	-0.012973614668744915
1101	22-23	0.010600884736447824
1101	24-25	-0.11893156593577459
1101	26-27	-0.05194206225943532
1101	28-29	-0.068799053482266
1101	30-31	-0.2051140355714196
1101	32-33	-0.28128850890438173
1101	34-35	-0.1795811338193758
1101	36-37	-0.23065066064508954
1101	38-39	-0.1685120408549281
1101	40-41	-0.2670695380522048
1101	42-43	-0.26648388911896603
1101	44-45	-0.2993236204912648
1101	46-47	-0.21320715389126121
1101	48-49	-0.33816880223835
1101	50-51	-0.31364002021278026
1101	52-53	-0.3024498973470102
1101	54-55	-0.1100849107177666
1101	56-57	-0.2282743983055866
1101	58-59	-0.25020122312234605
1101	60-61	-0.21897321728021524
1101	62-63	-0.2859606439856961
1101	64-65	-0.3129289959513457
1101	66-67	-0.3462974227381892
1101	68-69	-0.270970451995737
1101	70-71	-0.2544466990954888
1101	72-73	-0.3295195703192846
1101	74-75	-0.4466739515952618
1101	76-77	-0.2574104048755004
1101	78-79	-0.3884575342184142
1101	80-81	-0.4098990055194598
1101	82-83	-0.7732367809230958
1101	84-85	-0.5346422566963192
1101	86-87	-0.5341180784903372
1101	88-89	-0.739949355872195
1101	90-91	-0.5794482769223421
1101	92-93	-0.565324349358221
1101	94-95	-0.5223446869801975
1101	96-97	-0.4853008094319584
1101	98-99	-0.586926764107286
1101	100	-0.5555980500684257
1102	1	-0.058161576274745386
1102	2	-0.11265216031023328
1102	3	-0.06680414661010303
1102	4	0.046858605344574755
1102	5	-0.09009323840115968
1102	6	-0.007467478379041381
1102	7	0.02968955188087108
1102	8	0.049187500699453324
1102	9	-0.06069967200131998
1102	10-11	-0.048499483835961144
1102	12-13	-0.07455553933463932
1102	14-15	-0.14787369937371153
1102	16-17	-0.05994229120543082
1102	18-19	-0.08595582309592942
1102	20-21	-0.10343435343871477
1102	22-23	-0.013150558305738969
1102	24-25	-0.1457146092629955
1102	26-27	-0.16130059520656204
1102	28-29	-0.10556149013325467
1102	30-31	-0.26873334430433005
1102	32-33	-0.2492177487206959
1102	34-35	-0.18285180401088752
1102	36-37	-0.559573430357311
1102	38-39	-0.11880635270443207
1102	40-41	-0.8928187308390392
1102	42-43	-0.8249407080892794
1102	44-45	-0.29946271161082905
1102	46-47	-0.2393015045600464
1102	48-49	-0.3774176335460666
1102	50-51	-0.37984874571206007
1102	52-53	-0.38317143985624824
1102	54-55	-0.12886762221009462
1102	56-57	0.0966483182048492
1102	58-59	-0.06483694528366613
1102	60-61	-0.2562941600069095
1102	62-63	-0.4593264529538459
1102	64-65	-0.1899146231271729
1102	66-67	-0.19785135458715075
1102	68-69	-0.18316326520042026
1102	70-71	-0.2922998851500438
1102	72-73	-0.2161162360549227
1102	74-75	-0.1383345259807207
1102	76-77	-0.4320512533448557
1102	78-79	-0.5045685239723348
1102	80-81	-0.511796551045876
1102	82-83	-0.48954872014861905
1102	84-85	-0.616957824013376
1102	86-87	-0.5655826538758078
1102	88-89	-0.6006686485769421
1102	90-91	-0.5766495471827007
1102	92-93	-0.47887448449302283
1102	94-95	-0.5883539057682796
1102	96-97	-0.8060655581064786
1102	98-99	-0.5813460992607524
1102	100	-0.7707091166944231
1103	1	0.033852390524934606
1103	2	0.06586686946929277
1103	3	0.0769886843803711
1103	4	0.10624921679744403
1103	5	0.01551220997906455
1103	6	0.0878282405712767
1103	7	0.06998815490172916
1103	8	0.02551890014980529
1103	9	0.015213088455077184
1103	10-11	-0.018821493003947865
1103	12-13	0.07629283065691084
1103	14-15	0.06033240875260759
1103	16-17	-0.07568725201019788
1103	18-19	0.014504035070025623
1103	20-21	-0.0948535330462974
1103	22-23	-0.1170067535824515
1103	24-25	-0.06799664952151119
1103	26-27	-0.09825422240394488
1103	28-29	-0.30870125267013293
1103	30-31	-0.2613632576206655
1103	32-33	-0.18195701288779276
1103	34-35	-0.2838364568064904
1103	36-37	-0.3125221606183217
1103	38-39	-0.40067753856602906
1103	40-41	-0.2102026155863186
1103	42-43	-0.2052785946041027
1103	44-45	-0.25572173555040933
1103	46-47	-0.2220094224107072
1103	48-49	-0.29087304174675666
1103	50-51	-0.4060167867998885
1103	52-53	-0.18854851113647442
1103	54-55	-0.11638661249819648
1103	56-57	-0.01522856249935245
1103	58-59	-0.11318274833276121
1103	60-61	-0.03958325995551348
1103	62-63	-0.19376028806758683
1103	64-65	-0.24290437061543457
1103	66-67	-0.14231396159544119
1103	68-69	-0.1076106021920964
1103	70-71	-0.32173023185480787
1103	72-73	-0.18940481393888575
1103	74-75	-0.05152968720445372
1103	76-77	-0.13638783783944675
1103	78-79	-0.21651801504158996
1103	80-81	-0.1707543499735067
1103	82-83	-0.12410441023429541
1103	84-85	-0.2517507618102002
1103	86-87	-0.876980970344512
1103	88-89	-0.3638409451647
1103	90-91	-0.8721483403009032
1103	92-93	-0.4024261964162612
1103	94-95	-0.7476019068142001
1103	96-97	-0.5212253727121983
1103	98-99	-0.4510094842500614
1103	100	-0.559111907062583
1104	1	0.1344397607312402
1104	2	-0.04082666026688031
1104	3	0.03202580641461239
1104	4	0.057059556410685275
1104	5	0.03996471927440126
1104	6	0.06780680348600754
1104	7	7.069347372237189E-4
1104	8	0.05421983650271045
1104	9	0.11010717798678371
1104	10-11	0.10161156280363315
1104	12-13	0.10551108864912351
1104	14-15	0.09720745693416433
1104	16-17	0.034799722662612
1104	18-19	0.06505204465296544
1104	20-21	0.003406075096826555
1104	22-23	-0.061513092792445434
1104	24-25	-0.03772447562909065
1104	26-27	0.031675343576615944
1104	28-29	0.0202938135387285
1104	30-31	-0.06363475845383704
1104	32-33	0.025748848361189403
1104	34-35	0.05037215004981732
1104	36-37	0.0949681437704939
1104	38-39	-0.012655991775069708
1104	40-41	0.07648504341280926
1104	42-43	-0.07670078065309127
1104	44-45	0.010087077078196671
1104	46-47	-0.0451972029756007
1104	48-49	0.03222141485425567
1104	50-51	-0.13550678875679978
1104	52-53	-0.11308122418788713
1104	54-55	0.014273608217791889
1104	56-57	0.049282980318487546
1104	58-59	-0.051469886674610166
1104	60-61	-0.1319680541535675
1104	62-63	0.057739492521029945
1104	64-65	-0.057500787093822225
1104	66-67	-0.038766094333389844
1104	68-69	0.006056873924677575
1104	70-71	0.04922110922315426
1104	72-73	0.10727681124716781
1104	74-75	0.03054618169428025
1104	76-77	0.11653959925768476
1104	78-79	0.10092800679280955
1104	80-81	0.05688651806536882
1104	82-83	-0.010991837368962365
1104	84-85	0.025182652764648594
1104	86-87	0.03322062127753256
1104	88-89	0.09613656516838631
1104	90-91	0.046070884287406244
1104	92-93	0.053149076513712146
1104	94-95	-0.0061173664217513135
1104	96-97	-0.0704012457619605
1104	98-99	0.08197316149542999
1104	100	0.017155341933754897
1105	1	0.11222665879252958
1105	2	0.056176675151089484
1105	3	0.06074036759868662
1105	4	0.10415279176611847
1105	5	0.09496654333110399
1105	6	0.13091395378827997
1105	7	0.09062511276516005
1105	8	-6.738470495051274E-4
1105	9	-0.003467712569815262
1105	10-11	0.1030128292201411
1105	12-13	0.12038496815713984
1105	14-15	0.1277584523479689
1105	16-17	0.11100620588129573
1105	18-19	0.11757836318537329
1105	20-21	0.025219838907538872
1105	22-23	0.1589241596569977
1105	24-25	0.07172478953663841
1105	26-27	0.12227689141901976
1105	28-29	0.07394453273823842
1105	30-31	-0.034817268355858744
1105	32-33	0.016071576107698604
1105	34-35	0.05483262013529355
1105	36-37	0.0294779936766858
1105	38-39	-0.06690148374921989
1105	40-41	-0.06751771873019408
1105	42-43	-0.028244062912840207
1105	44-45	-0.06436114576941065
1105	46-47	-0.10191559467874356
1105	48-49	-0.09547623471839017
1105	50-51	-0.1799766397053162
1105	52-53	-0.04812982227002749
1105	54-55	0.08457796510751336
1105	56-57	0.08967801888425697
1105	58-59	0.04959848939377309
1105	60-61	0.01489456831881597
1105	62-63	-0.0026387386651265388
1105	64-65	-0.05034331888452215
1105	66-67	0.07848687627323869
1105	68-69	0.08882214388506782
1105	70-71	0.05771860765968029
1105	72-73	0.08859651627114573
1105	74-75	0.009184523366155162
1105	76-77	0.0021758041774262438
1105	78-79	0.025131936332101645
1105	80-81	0.10372829417543983
1105	82-83	0.03299333565584561
1105	84-85	-0.027118264475298304
1105	86-87	-0.023091917809395568
1105	88-89	-0.08604826389144193
1105	90-91	-0.13169972755332893
1105	92-93	-0.12285415467244576
1105	94-95	-0.1407496887065136
1105	96-97	-0.11594468436942051
1105	98-99	-0.0634315185129033
1105	100	-0.17966949650693564
1106	1	0.031875591777449586
1106	2	0.003356706726023617
1106	3	0.003941208689653308
1106	4	-8.219618949425467E-4
1106	5	5.700737355880392E-4
1106	6	0.03591694796325129
1106	7	0.08865508173462189
1106	8	-1.0978144099544025
1106	9	0.016366995362027126
1106	10-11	-0.017927171596447522
1106	12-13	-0.05761873373190696
1106	14-15	-0.11957486057054467
1106	16-17	-0.11871166117733623
1106	18-19	-0.021523180175719858
1106	20-21	-0.02852304992691046
1106	22-23	0.003968800083804069
1106	24-25	0.0017929410418915381
1106	26-27	0.041531988321409585
1106	28-29	-0.1061278718564651
1106	30-31	-0.10947089675152455
1106	32-33	-0.0903755971189355
1106	34-35	-0.054086448676294197
1106	36-37	0.05312346975050275
1106	38-39	-0.017602222766036846
1106	40-41	-0.06997940690047955
1106	42-43	-0.07077410673667117
1106	44-45	-0.1371187468908701
1106	46-47	-0.04991817375218943
1106	48-49	-0.08606414777843696
1106	50-51	-0.1605255604413358
1106	52-53	-0.01735361643411437
1106	54-55	0.011261508033101109
1106	56-57	-0.10851935168572169
1106	58-59	0.06945191091917025
1106	60-61	0.06808200367185435
1106	62-63	-0.013466253772101311
1106	64-65	0.014859094148299334
1106	66-67	0.012084241399257678
1106	68-69	-0.02856652724563702
1106	70-71	-0.028152969453941523
1106	72-73	0.04266229965619317
1106	74-75	0.016311850656702376
1106	76-77	0.152491043167025
1106	78-79	0.11220288466897088
1106	80-81	-0.020788554863703013
1106	82-83	0.17535359614906554
1106	84-85	0.14103868473261016
1106	86-87	-0.07051386511191637
1106	88-89	0.0642805849843846
1106	90-91	0.038302211855459234
1106	92-93	0.060021886483848874
1106	94-95	-0.046982676348736874
1106	96-97	-0.01365703261918938
1106	98-99	0.07943315838839382
1106	100	0.1168882230205277
1107	1	-0.1059575469298153
1107	2	-0.19292389336444415
1107	3	-0.15466248869864074
1107	4	-0.05497011655253203
1107	5	-0.13373191156296116
1107	6	-0.07696300239724962
1107	7	0.006036175347226447
1107	8	0.016719791563289732
1107	9	-0.06524991068625496
1107	10-11	-0.013292867217053583
1107	12-13	0.001145241461856017
1107	14-15	-0.0848786096150107
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2316	94-95	0.4832634906501809
2316	96-97	0.5338654953787376
2316	98-99	0.5377604140244436
2316	100	0.08051269831266339
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	6715.0
1	603.0
2	369.0
3	282.0
4	236.0
5	260.0
6	293.0
7	1667.0
8	3355.0
9	3947.0
10	4606.0
11	6041.0
12	7920.0
13	9465.0
14	11009.0
15	12999.0
16	15299.0
17	19151.0
18	24245.0
19	29184.0
20	33938.0
21	40325.0
22	46192.0
23	50114.0
24	51279.0
25	52670.0
26	55502.0
27	61271.0
28	70091.0
29	82968.0
30	101132.0
31	125512.0
32	160617.0
33	214948.0
34	313336.0
35	502519.0
36	564548.0
37	545679.0
38	484865.0
39	101205.0
40	136.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.19254819740271	16.644983192902448	11.84786231037894	23.31460629931591
2	17.070078558467845	19.962923339941057	39.24443850099116	23.722559600599936
3	19.538324501729043	26.441957291323614	29.379607943528896	24.640110263418443
4	13.188693115559122	15.713243045555386	35.85931128520129	35.23875255368421
5	13.334696546619792	35.92861633500984	33.054225050042895	17.682462068327474
6	30.327089607815584	33.974894628101886	19.911657041233283	15.78635872284925
7	28.22133980331562	31.994889022220775	21.78317853641519	18.000592638048413
8	27.73560203235139	32.54441631366006	20.76902991524164	18.950951738746912
9	28.784222237985276	15.483754863557667	20.188105728029594	35.54391717042747
10-11	26.081470367099293	24.81886682185457	27.97395732442042	21.125705486625716
12-13	26.265490189382547	23.151008249613525	27.80033072005821	22.78317084094572
14-15	23.836346184400405	24.185204005929847	26.55886319200705	25.4195866176627
16-17	22.99756773175031	26.5002730097015	27.22418863328901	23.27797062525919
18-19	22.721293424447975	25.736235090904508	29.169910629481134	22.372560855166384
20-21	23.680928463712807	25.20275273530678	28.96995437587356	22.14636442510685
22-23	23.355439193257588	24.93300150248407	28.880735385909638	22.830823918348706
24-25	23.026033092148047	24.853163967821885	28.976746824790638	23.14405611523943
26-27	22.754065198132363	24.769638115879715	29.21424423468218	23.26205245130574
28-29	22.717468879918737	25.6683767859127	28.975515025557225	22.638639308611335
30-31	23.85087244475996	25.046467266479826	28.967918479198655	22.134741809561554
32-33	23.417141723891337	25.093922441504095	28.992651032736426	22.49628480186814
34-35	23.3671986864263	25.41219888077302	28.796431794282423	22.42417063851826
36-37	23.394316798576227	25.426048372047333	28.677317925214783	22.502316904161653
38-39	23.384250701774544	25.762581873697	28.332399822709586	22.520767601818868
40-41	23.355473702723607	25.466648050350216	28.50172058634041	22.676157660585762
42-43	23.059386636555594	25.71891374243316	28.033019746340983	23.188679874670264
44-45	22.85208928060256	25.688408175913985	28.363819243284127	23.09568330019933
46-47	22.979354890557993	25.453372213130837	28.087935470671678	23.4793374256395
48-49	23.0289375284327	25.651338661433126	28.100123028884997	23.219600781249177
50-51	22.78904123260526	26.06579830310607	28.132983326526674	23.012177137761995
52-53	22.894721565308437	26.318203345834178	27.478583209677687	23.308491879179698
54-55	22.589650477219035	25.937697111781244	28.004394024693863	23.46825838630586
56-57	22.978675728189515	26.001055764958718	27.476854408856344	23.543414097995427
58-59	22.905467163513173	26.099467283561207	27.75731225846736	23.237753294458262
60-61	23.41894744361868	26.099885200594297	27.87578289333898	22.605384462448043
62-63	23.175792139254785	26.068513487561713	27.90363051182038	22.85206386136312
64-65	22.966954447601147	25.910266463341753	27.68678506271155	23.43599402634555
66-67	22.94137072153595	25.858911008788443	27.5696398533342	23.63007841634141
68-69	22.57163747606567	25.915521006513504	27.772966974301198	23.73987454311963
70-71	22.748806199040754	25.83073358188639	27.828110005224026	23.592350213848825
72-73	22.659730920302604	26.33751436880538	27.336024796077286	23.66672991481473
74-75	22.730788234841256	26.479135029636495	27.24922287695739	23.54085385856485
76-77	22.649135365573134	25.915070832984654	27.44639066870326	23.98940313273895
78-79	22.61576522865095	26.08113250847859	27.49632146766642	23.80678079520404
80-81	22.813378826249657	26.14543679228444	27.16297237023794	23.878212011227962
82-83	22.9707444397884	26.25388009568385	27.204492044079725	23.570883420448023
84-85	22.984399119781916	26.50745229415049	26.799119781916115	23.709028804151476
86-87	22.693856242944833	26.4656848120989	26.98190002034857	23.8585589246077
88-89	22.44465893730393	26.125282835635893	27.271089852757964	24.158968374302216
90-91	23.29661506351124	25.865571511821294	27.23261460950296	23.605198815164506
92-93	23.13094021144306	26.029025907442144	27.17928423846494	23.660749642649854
94-95	22.939471674595502	25.799343649292595	27.132657014388563	24.128527661723346
96-97	22.767398360110246	25.78562888866907	27.332498636096307	24.114474115124377
98-99	22.883531289104567	25.823124251566416	27.006485012414295	24.286859446914725
100	22.87142847008022	25.90010003082116	27.12616732184621	24.102304177252407
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4130.0
1	4207.5
2	6034.5
3	6111.5
4	3201.0
5	2164.0
6	2893.5
7	4957.5
8	7537.0
9	8634.5
10	7377.5
11	5866.5
12	5863.0
13	6241.0
14	6716.0
15	7084.0
16	7335.0
17	7745.0
18	8280.0
19	8740.5
20	9018.0
21	9550.0
22	10508.5
23	11823.5
24	13380.5
25	15302.5
26	18185.5
27	21186.0
28	23803.0
29	27413.0
30	32140.5
31	36513.5
32	40891.0
33	46236.0
34	52323.0
35	57828.5
36	64431.0
37	71600.0
38	76567.5
39	81402.5
40	85726.5
41	89273.5
42	93824.0
43	100605.0
44	107499.5
45	114612.5
46	122953.5
47	133778.0
48	151029.0
49	163860.0
50	170264.5
51	168847.0
52	160295.5
53	155715.5
54	151637.0
55	143875.0
56	133272.5
57	122820.5
58	112226.5
59	99340.5
60	82782.0
61	66733.5
62	54957.5
63	43941.0
64	33399.0
65	25372.0
66	19264.0
67	15184.0
68	11927.0
69	9130.5
70	7322.0
71	5823.0
72	4687.5
73	3828.5
74	3022.0
75	2289.5
76	1722.5
77	1214.0
78	838.5
79	613.0
80	449.5
81	320.0
82	237.0
83	184.0
84	149.0
85	112.5
86	79.5
87	62.5
88	52.5
89	46.5
90	34.5
91	24.0
92	18.5
93	14.0
94	9.5
95	8.5
96	8.5
97	7.5
98	5.5
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1468101736332282
2	0.07265832794662534
3	0.30881754532236794
4	0.3064593594171403
5	0.30805768541957235
6	0.25515571494563205
7	0.2563872120294731
8	0.2534001765495181
9	0.26524351020688364
10-11	0.24316826992739146
12-13	0.25279752904040437
14-15	0.22633344277062736
16-17	0.2341416583234923
18-19	0.24684966014610793
20-21	0.24933885637940378
22-23	0.239264162150959
24-25	0.24066597266128878
26-27	0.2388056260027203
28-29	0.25012491834781303
30-31	0.23513733681681062
32-33	0.24880171403432416
34-35	0.2322813116649238
36-37	0.23973579933200453
38-39	0.24054806336602738
40-41	0.25668853578403
42-43	0.24252631931985727
44-45	0.22856061834778682
46-47	0.23215030133685557
48-49	0.2425525213854709
50-51	0.23445608311085595
52-53	0.2502297266102676
54-55	0.26097257351186026
56-57	0.2561120903405299
58-59	0.2537277023696886
60-61	0.23534695334171976
62-63	0.2635927800732243
64-65	0.25620379757017764
66-67	0.26243988918622413
68-69	0.2684401622117478
70-71	0.2882489238156601
72-73	0.2980484963551617
74-75	0.278488654374579
76-77	0.27413911148271464
78-79	0.26955375000032755
80-81	0.25537843250334796
82-83	0.2674706857840431
84-85	0.27821353268563576
86-87	0.2706280346904868
88-89	0.27817422958721527
90-91	0.2723704720537939
92-93	0.25291543833566577
94-95	0.27377228256412367
96-97	0.27047082229680497
98-99	0.25658372752157543
100	0.2794712318350905
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3816493.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.93367394754766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.42760894258655	44.27732177070487
2	14.380516832251331	16.66232346713739
3	4.1584699740460795	7.227463307911576
4	1.6684821471550382	3.866452028023369
5	0.8605339272301449	2.492694598047696
6	0.5300904886591657	1.8426053719605793
7	0.35972315574351404	1.4588058811359355
8	0.26234737601453834	1.215899787441676
9	0.18258254964683002	0.9519910109776245
>10	1.0767551575549998	11.065409641008012
>50	0.06206150628513425	2.435991409424915
>100	0.028008464541725387	2.873966795835193
>500	0.0014895356997919112	0.5953072234293351
>1k	0.0010639540682367608	1.174680822768365
>5k	1.0639540682367609E-4	0.4413027601002287
>10k+	1.5959311023551413E-4	1.4177841240932203
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19347	0.5069313634271044	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16089	0.42156503365786335	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10567	0.27687722733934006	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8839	0.23160005795896912	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	5480	0.1435873195627504	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	4512	0.11822372004874633	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4400	0.11528908870001857	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	2.6202065613640586E-5	0.0
7	0.0	0.0	0.0	2.6202065613640586E-5	0.0
8	0.0	0.0	0.0	2.6202065613640586E-5	0.0
9	0.0	0.0	0.0	2.6202065613640586E-5	5.240413122728117E-5
10-11	0.0	0.0	0.0	5.240413122728117E-5	5.240413122728117E-5
12-13	0.0	0.0	0.0	5.240413122728117E-5	5.240413122728117E-5
14-15	0.0	0.0	0.0	5.240413122728117E-5	5.240413122728117E-5
16-17	0.0	0.0	0.0	6.550516403410146E-5	7.860619684092176E-5
18-19	0.0	0.0	0.0	1.1790929526138264E-4	1.3101032806820293E-4
20-21	0.0	0.0	0.0	2.0961652490912466E-4	1.834144592954841E-4
22-23	0.0	0.0	0.0	4.0613201701142907E-4	1.834144592954841E-4
24-25	0.0	0.0	0.0	9.301733292842408E-4	2.096165249091247E-4
26-27	0.0	0.0	0.0	0.0018079425273412003	2.3581859052276526E-4
28-29	0.0	0.0	0.0	0.005305918286762219	2.7512168894322617E-4
30-31	0.0	0.0	0.0	0.016743119927116336	3.1442478736368705E-4
32-33	0.0	0.0	0.0	0.0437050454435525	3.406268529773276E-4
34-35	0.0	0.0	0.0	0.08764590947762776	3.406268529773276E-4
36-37	0.0	0.0	0.0	0.15186717229666083	3.406268529773276E-4
38-39	0.0	0.0	0.0	0.25237829599058614	3.668289185909682E-4
40-41	0.0	0.0	0.0	0.38034918444760674	4.0613201701142907E-4
42-43	0.0	2.6202065613640586E-5	0.0	0.4941971595388751	4.4543511543188995E-4
44-45	0.0	2.6202065613640586E-5	0.0	0.6325309649460906	4.716371810455305E-4
46-47	0.0	2.6202065613640586E-5	0.0	0.7886952760033885	4.716371810455305E-4
48-49	0.0	2.6202065613640586E-5	0.0	0.9606725336585185	5.240413122728117E-4
50-51	0.0	2.6202065613640586E-5	0.0	1.1396981469637177	5.633444106932726E-4
52-53	0.0	2.6202065613640586E-5	0.0	1.3199814594183719	6.288495747273741E-4
54-55	0.0	2.6202065613640586E-5	0.0	1.52847129550611	6.943547387614755E-4
56-57	0.0	2.6202065613640586E-5	0.0	1.7631893992731023	7.205568043751161E-4
58-59	0.0	2.6202065613640586E-5	0.0	2.0320487945346684	7.59859902795577E-4
60-61	0.0	3.930309842046088E-5	0.0	2.30566386470511	7.860619684092176E-4
62-63	0.0	5.240413122728117E-5	0.0	2.595917246540214	8.646681652501393E-4
64-65	0.0	5.240413122728117E-5	0.0	2.87589941865477	8.646681652501393E-4
66-67	0.0	5.240413122728117E-5	0.0	3.1990101907693793	8.646681652501393E-4
68-69	0.0	5.240413122728117E-5	0.0	3.5753373581452923	8.646681652501393E-4
70-71	0.0	5.240413122728117E-5	0.0	3.9790456840874597	8.908702308637799E-4
72-73	0.0	5.240413122728117E-5	0.0	4.336022626007699	9.694764277047017E-4
74-75	0.0	5.240413122728117E-5	0.0	4.698646113067678	9.694764277047017E-4
76-77	0.0	5.240413122728117E-5	0.0	5.109022864708516	9.694764277047017E-4
78-79	0.0	5.240413122728117E-5	0.0	5.566799153044431	9.694764277047017E-4
80-81	0.0	5.240413122728117E-5	0.0	6.021391890408289	9.956784933183422E-4
82-83	0.0	6.550516403410146E-5	0.0	6.490042035973865	0.001074284690159264
84-85	0.0	7.860619684092176E-5	0.0	6.970378302803123	0.001074284690159264
86-87	0.0	7.860619684092176E-5	0.0	7.4678900236421235	0.0010873857229660843
88	0.0	7.860619684092176E-5	0.0	7.873615908636541	0.0011004867557729047
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	8595	0.0	61.231113	2
ACCTGGG	9460	0.0	54.935535	3
GTACCTG	10055	0.0	52.768875	1
CCTGGGG	8555	0.0	41.72025	4
TATAACG	495	0.0	38.955307	2
GTACCCG	890	0.0	35.41079	1
CTGGGGG	5450	0.0	35.201424	5
ATAACGC	675	0.0	30.657106	3
TAACGCA	700	0.0	28.214764	4
TGGGGGG	8680	0.0	27.82514	6
TACCCGG	1065	0.0	26.938179	2
TATCACG	430	0.0	25.156336	2
CATGGGG	10750	0.0	25.021461	4
GTATAAC	1080	0.0	24.390186	1
ATGGGGG	7170	0.0	24.068193	5
GTATAGG	865	0.0	23.383158	1
GTACATG	19570	0.0	22.52162	1
GGTATCA	20225	0.0	22.07133	1
GTATCAA	26145	0.0	22.039324	1
TACATGG	19970	0.0	21.902458	2
>>END_MODULE