FastQCFastQC Report
Mon 6 Jun 2016
ERR523125_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523125_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3816493
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169810.44493727618523077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155250.40678706865177006No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101140.2650076916163609No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74690.19570322806828153No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT56830.14890633888231944No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA43490.1139527833537229No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40180.10527989963560788No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG86150.062.572932
ACCTGGG96100.055.1578333
GTACCTG102350.053.3814281
CCTGGGG83650.044.716584
CTGGGGG50150.039.72985
TATAACG4950.038.9275472
TAACGCA5550.037.2547074
ATAACGC5300.037.238733
GTACCCG9250.035.0735131
TGGGGGG76150.031.1632656
TACCGTA3450.029.9657427
TACCCGG10800.027.8506012
GCGTAAT700.00882128826.8679081
GTATAAC10050.026.1995531
GTACACG8100.026.1215761
ATGGGGG70300.024.9998685
CATGGGG108300.024.5155414
TATCACG4050.024.3692762
GTACATG194200.022.6620161
ACCGTAT4500.021.9352238
Produced by FastQC (version 0.11.5)