##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR523125_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3816493
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.227409037564065	33.0	31.0	34.0	30.0	34.0
2	32.345162692555704	34.0	31.0	34.0	30.0	34.0
3	32.5368313265608	34.0	31.0	34.0	30.0	35.0
4	35.640259788240144	37.0	35.0	37.0	33.0	37.0
5	35.57880808375648	36.0	35.0	37.0	33.0	37.0
6	35.705135054616896	37.0	35.0	37.0	33.0	37.0
7	35.54998974189131	37.0	35.0	37.0	33.0	37.0
8	35.538797791585104	36.0	35.0	37.0	33.0	37.0
9	36.82764910088922	38.0	37.0	39.0	33.0	39.0
10-11	36.62769393262349	37.5	35.0	39.0	33.0	39.0
12-13	36.62287720690173	37.5	35.0	39.0	33.0	39.0
14-15	37.78561456813887	38.0	37.0	41.0	33.0	41.0
16-17	37.59763898427168	38.0	36.5	41.0	33.0	41.0
18-19	37.51499452507839	38.0	36.0	41.0	32.0	41.0
20-21	37.53169938998971	38.0	36.5	41.0	33.0	41.0
22-23	37.38071077295308	38.0	36.0	41.0	33.0	41.0
24-25	37.30426794965955	38.0	36.5	41.0	32.0	41.0
26-27	36.98539680277155	38.0	36.0	40.0	32.5	41.0
28-29	36.944997147905156	38.0	36.0	40.0	32.0	41.0
30-31	36.6850057107402	38.0	35.5	40.0	31.5	41.0
32-33	36.59764802398432	37.5	36.0	40.0	30.5	41.0
34-35	36.34814566671549	37.5	35.0	40.0	30.5	41.0
36-37	36.1193488367462	37.0	35.0	40.0	30.0	41.0
38-39	36.06769735985367	37.0	35.0	40.0	30.0	41.0
40-41	36.24665471677794	37.5	35.0	40.0	30.0	41.0
42-43	36.2759766623442	37.5	35.0	40.0	30.5	41.0
44-45	36.0746139191137	37.0	35.0	40.0	30.0	41.0
46-47	35.93375515165363	37.0	35.5	40.0	29.0	41.0
48-49	35.6937802584729	37.5	35.0	40.0	28.5	41.0
50-51	35.5136895312005	37.5	34.5	40.0	28.0	41.0
52-53	35.52283365906868	37.5	35.0	40.0	27.5	41.0
54-55	35.3292453307264	37.5	34.0	40.0	27.0	41.0
56-57	35.078458810221846	37.0	34.5	40.0	26.5	41.0
58-59	34.89181966271129	37.0	34.0	39.5	26.5	41.0
60-61	34.69798962031373	36.5	34.0	39.0	26.0	41.0
62-63	34.439350602765415	36.5	34.0	39.0	26.0	41.0
64-65	34.2511181076449	36.5	33.0	38.5	25.0	40.5
66-67	33.75119684485207	35.5	33.5	38.0	25.0	40.0
68-69	33.60165720204387	35.5	33.0	37.0	24.0	40.0
70-71	33.18242991668005	35.0	32.5	37.0	24.0	39.5
72-73	32.9441725689003	35.0	32.5	37.0	23.5	39.0
74-75	32.75314365832716	35.0	32.0	37.0	22.5	39.0
76-77	31.161379570197035	34.0	29.5	36.0	21.0	37.0
78-79	32.271219939352704	35.0	31.5	36.5	23.0	37.0
80-81	32.38863938699743	35.0	32.0	36.0	23.0	37.5
82-83	32.28939028579379	35.0	32.0	37.0	23.5	37.0
84-85	31.963580307889995	35.0	32.0	36.0	22.0	36.5
86-87	31.84142234768936	35.0	32.0	36.0	21.5	36.0
88-89	31.809622734798676	35.0	31.5	36.0	22.0	37.0
90-91	31.568941171908346	34.5	31.0	35.0	21.5	36.5
92-93	31.35365517505207	35.0	31.0	35.0	21.0	36.5
94-95	31.48985246402915	34.0	31.0	35.0	20.0	36.5
96-97	31.222175698999052	34.0	31.0	35.0	20.0	37.0
98-99	31.073745582659264	34.0	31.0	35.0	20.0	36.0
100	29.852069426041133	34.0	29.0	35.0	18.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.058195622419688675
1101	2	-0.17398595458235633
1101	3	-0.1554515797689291
1101	4	-0.10098899330510847
1101	5	-0.18280979711913403
1101	6	-0.09572447887317281
1101	7	-0.1372337985601817
1101	8	-0.23292437594288629
1101	9	-0.1818330951144489
1101	10-11	-0.19272436729430353
1101	12-13	-0.19463088204226864
1101	14-15	-0.3781622134460392
1101	16-17	-0.38197208324039167
1101	18-19	-0.5048414084020081
1101	20-21	-0.44090651004010084
1101	22-23	-0.9482979256293831
1101	24-25	-0.4345560820216505
1101	26-27	-0.3196258110166994
1101	28-29	-0.4069409670809563
1101	30-31	-0.40868527733825744
1101	32-33	-0.44881427005905294
1101	34-35	-0.4982120173020945
1101	36-37	-0.5238853706816471
1101	38-39	-0.5475514040384368
1101	40-41	-0.5256633119496286
1101	42-43	-0.6303924940512573
1101	44-45	-0.6664628709811069
1101	46-47	-0.5368630600770317
1101	48-49	-0.6603384266165762
1101	50-51	-0.5248464330231499
1101	52-53	-0.4960770483709567
1101	54-55	-0.6529608154503492
1101	56-57	-0.7662408826956408
1101	58-59	-0.6335479950294527
1101	60-61	-0.7763304467419303
1101	62-63	-0.692327520675164
1101	64-65	-0.6443294893884683
1101	66-67	-0.5615321588714153
1101	68-69	-0.6299344157340485
1101	70-71	-0.7159263223118515
1101	72-73	-0.7212002296251896
1101	74-75	-0.6884857080123439
1101	76-77	-0.6890891410751543
1101	78-79	-0.6536023760764778
1101	80-81	-0.559493566641251
1101	82-83	-0.5588180828495481
1101	84-85	-0.4939475449670283
1101	86-87	-0.6461481453752604
1101	88-89	-0.5609033480749588
1101	90-91	-0.7036375360977232
1101	92-93	-0.509222987283529
1101	94-95	-0.5514843460796222
1101	96-97	-0.7764959203564139
1101	98-99	-0.8011544888393836
1101	100	-0.6611663743292908
1102	1	-0.016622706999939396
1102	2	-0.14555343034454893
1102	3	-0.11166718317753777
1102	4	-0.06454779627385676
1102	5	-0.1111999628134086
1102	6	-0.13812269832649093
1102	7	-0.10042627564678241
1102	8	-0.1097239095705902
1102	9	-0.12077164276420405
1102	10-11	-0.1613721694179091
1102	12-13	-0.1587248589459307
1102	14-15	-0.22689363255192774
1102	16-17	-0.235338708269893
1102	18-19	-0.252744502694739
1102	20-21	-0.29187623664376616
1102	22-23	-0.23931070059641257
1102	24-25	-0.1750604079358169
1102	26-27	-0.2669342851375234
1102	28-29	-0.36744382318296687
1102	30-31	-0.382661937532788
1102	32-33	-0.3669342360369612
1102	34-35	-0.35084170737236775
1102	36-37	-0.47180803241226954
1102	38-39	-0.37230290546733613
1102	40-41	-0.4695811864209034
1102	42-43	-0.44347589619749783
1102	44-45	-0.27122779008169573
1102	46-47	-0.28271642016455445
1102	48-49	-0.4552149815572193
1102	50-51	-0.30041118918198606
1102	52-53	-0.4277345027870538
1102	54-55	-0.28438783397922407
1102	56-57	-0.3949342084184053
1102	58-59	-0.40306600710400886
1102	60-61	-0.4660639215559499
1102	62-63	-0.589860648370049
1102	64-65	-0.4990671876581203
1102	66-67	-0.5375356719929769
1102	68-69	-0.4808599578005541
1102	70-71	-0.4315884557802576
1102	72-73	-0.4218629525964772
1102	74-75	-0.5216929125265608
1102	76-77	-0.4227432857087301
1102	78-79	-0.5368233206291677
1102	80-81	-0.39879812456931774
1102	82-83	-0.33253272423003466
1102	84-85	-0.5018289617847884
1102	86-87	-0.5217358729848023
1102	88-89	-0.6099287972412135
1102	90-91	-0.6016033791961775
1102	92-93	-0.5249523443692077
1102	94-95	-0.7283554992351746
1102	96-97	-0.7823286174098705
1102	98-99	-0.6606748251107604
1102	100	-0.7101873148693016
1103	1	-0.03188352390992577
1103	2	-0.06918681770932977
1103	3	-0.17199923124458394
1103	4	-0.11061528831125145
1103	5	-0.12064544340905314
1103	6	-0.08271473697543286
1103	7	-0.1344942092700805
1103	8	-0.08034034673823953
1103	9	-0.12112422337906281
1103	10-11	-0.26285816881117796
1103	12-13	-0.15066599099747435
1103	14-15	-0.2554610767209269
1103	16-17	-0.2575335607425018
1103	18-19	-0.3484073497630078
1103	20-21	-0.2840173314178003
1103	22-23	-0.3809576813874216
1103	24-25	-0.26747781551541294
1103	26-27	-0.28520325642786304
1103	28-29	-0.2894704780979396
1103	30-31	-0.46792055249680686
1103	32-33	-0.2998597381614232
1103	34-35	-0.4742048327953228
1103	36-37	-0.4896859413893324
1103	38-39	-0.3871602068030384
1103	40-41	-0.3669353508811355
1103	42-43	-0.3445090456563733
1103	44-45	-0.29385057848725893
1103	46-47	-0.3851103482985465
1103	48-49	-0.47566757419900796
1103	50-51	-0.3845988236996405
1103	52-53	-0.35317211711024044
1103	54-55	-1.0832000920568632
1103	56-57	-0.44028864842106685
1103	58-59	-0.3980092351926956
1103	60-61	-0.3924263681453013
1103	62-63	-0.40185731972183447
1103	64-65	-0.322988436077722
1103	66-67	-0.21568001942414838
1103	68-69	-0.2600962505372948
1103	70-71	-0.40129890886056074
1103	72-73	-0.4183039377518938
1103	74-75	-0.549941134365433
1103	76-77	-0.3666062289326284
1103	78-79	-0.30385710045491265
1103	80-81	-1.1738295905567284
1103	82-83	-0.17021131072432638
1103	84-85	-0.24281515684010202
1103	86-87	-0.23622706340585964
1103	88-89	-0.187694363593085
1103	90-91	-0.3155105378541663
1103	92-93	-0.3020806702500316
1103	94-95	-0.2989907696227725
1103	96-97	-0.37917512262842124
1103	98-99	-0.3233101505470053
1103	100	-0.2968578229743173
1104	1	-0.02795413286546733
1104	2	-0.144057246391732
1104	3	-0.07320080507651738
1104	4	-0.0222847124453196
1104	5	0.004132921442426607
1104	6	-0.09255622707523514
1104	7	-0.1504999732664487
1104	8	-0.046056724908588365
1104	9	-0.1049076465524621
1104	10-11	-0.1254418564642492
1104	12-13	-0.17167507581479668
1104	14-15	-0.2688705962100002
1104	16-17	-0.24786863750684063
1104	18-19	-0.23349486229021466
1104	20-21	-0.24124241488307518
1104	22-23	-0.2775424245871534
1104	24-25	-0.10040583079118903
1104	26-27	-0.19833947531739682
1104	28-29	-0.2812183862772457
1104	30-31	-0.28109516837863424
1104	32-33	-0.16108166684679048
1104	34-35	-0.23742318270311102
1104	36-37	-0.2569207797661548
1104	38-39	-0.13585204054851374
1104	40-41	-0.026766759917329352
1104	42-43	-0.03484516017725525
1104	44-45	-0.08128356906443202
1104	46-47	-0.07021705932188382
1104	48-49	-0.08690484685620703
1104	50-51	-0.03291261755968122
1104	52-53	0.009581836742214023
1104	54-55	0.02133067890729734
1104	56-57	-0.0362831305212481
1104	58-59	-0.07084091701060657
1104	60-61	-0.1597559388698926
1104	62-63	-0.18978628380360618
1104	64-65	-0.41235965938513885
1104	66-67	-0.2535900815116463
1104	68-69	-0.1290170726776907
1104	70-71	0.012348331003522617
1104	72-73	-0.07007108890261549
1104	74-75	-0.14836189127541388
1104	76-77	-0.09613722227319599
1104	78-79	0.02349957054067886
1104	80-81	-0.10435468054504327
1104	82-83	-0.0485906907982212
1104	84-85	-0.7151074276293343
1104	86-87	-0.09180819224778247
1104	88-89	-0.16053582997273708
1104	90-91	-0.17410449633204195
1104	92-93	-0.10653005145062622
1104	94-95	-0.27117281584148145
1104	96-97	-0.09619388526681405
1104	98-99	-0.14234779442454126
1104	100	-1.0501267561012853
1105	1	-0.05133463109352476
1105	2	-0.14958153240382188
1105	3	-0.16323363809715374
1105	4	-0.1289763947467577
1105	5	-0.08333059742353299
1105	6	-0.0864990559266019
1105	7	-0.1458173591326144
1105	8	-0.1458052656632276
1105	9	-0.1581258037340305
1105	10-11	-0.19853702010819063
1105	12-13	-0.14825483920858318
1105	14-15	-0.23303569939949398
1105	16-17	-0.1348950342131161
1105	18-19	-0.22906045015763254
1105	20-21	-0.17160318724079815
1105	22-23	-0.2744499970282632
1105	24-25	-0.19458774136029433
1105	26-27	-0.256691700666579
1105	28-29	-0.21910990701937294
1105	30-31	-0.3157874239550367
1105	32-33	-0.21282624679258788
1105	34-35	-0.28771242598016045
1105	36-37	-0.34347943621811083
1105	38-39	-0.25740131613741823
1105	40-41	-0.3349033557063663
1105	42-43	-0.2649460044549343
1105	44-45	-0.38937000329415383
1105	46-47	-0.4911455041832582
1105	48-49	-0.50158329171758
1105	50-51	-0.3866213157043532
1105	52-53	-0.49282722100220866
1105	54-55	-1.3663022166524286
1105	56-57	-0.46625433648330983
1105	58-59	-0.3896892857827581
1105	60-61	-0.4030414558596789
1105	62-63	-0.37861458795202196
1105	64-65	-0.3324594613447047
1105	66-67	-0.4504018909758898
1105	68-69	-0.3135933703637406
1105	70-71	-0.301048062575795
1105	72-73	-0.33826866030140224
1105	74-75	-0.3965678012191276
1105	76-77	-0.4175685856252933
1105	78-79	-0.4126330383815642
1105	80-81	-0.30483219253170546
1105	82-83	-0.17358417630998701
1105	84-85	-0.29096864297225267
1105	86-87	-0.18449177052587373
1105	88-89	-0.325861163514535
1105	90-91	-0.30716487260888314
1105	92-93	-0.28102583006225856
1105	94-95	-0.4127567485077286
1105	96-97	-0.34892541017821443
1105	98-99	-0.16995814985501667
1105	100	-0.164123785494656
1106	1	-0.07711005436098617
1106	2	-0.12161321621894672
1106	3	-0.0857355434812277
1106	4	-0.07597949780081592
1106	5	-0.13584774588017012
1106	6	-0.1002875719046088
1106	7	-0.09370850726261892
1106	8	-0.20667718302399862
1106	9	-0.09155644775385241
1106	10-11	-0.18004458720493233
1106	12-13	-0.15405860506410107
1106	14-15	-0.21146743221020614
1106	16-17	-0.16991242084276337
1106	18-19	-0.25685438967529706
1106	20-21	-0.3195486047921108
1106	22-23	-0.1860699477604726
1106	24-25	-0.1867475962789058
1106	26-27	-0.12114318822713699
1106	28-29	-0.09324823109798075
1106	30-31	-0.1981316588072346
1106	32-33	-0.06528997644580414
1106	34-35	-0.043190405184262204
1106	36-37	-0.15595306706355672
1106	38-39	-0.11677461490164376
1106	40-41	-0.2654470026114808
1106	42-43	-0.1772421964087627
1106	44-45	-0.19753100144320257
1106	46-47	-0.25522323024346605
1106	48-49	-0.2085352215994405
1106	50-51	-0.19805208901809124
1106	52-53	-0.29201505104195036
1106	54-55	-0.23123090084862952
1106	56-57	-0.020449754034615353
1106	58-59	-0.11806572843070029
1106	60-61	-0.13042097434190225
1106	62-63	-0.17392615227235098
1106	64-65	-0.09568997198763185
1106	66-67	-0.16377598591845555
1106	68-69	-0.03856819207423001
1106	70-71	-0.06733229143964081
1106	72-73	-0.016954557737300036
1106	74-75	-0.06864609889456119
1106	76-77	-0.16579419670918227
1106	78-79	0.034986762641899816
1106	80-81	-0.012606669912074153
1106	82-83	-0.02165871765682681
1106	84-85	0.14454941734676652
1106	86-87	0.08746182656705415
1106	88-89	0.004325734248002533
1106	90-91	-1.0457400797747596E-4
1106	92-93	0.060002192693470846
1106	94-95	0.1685189401525129
1106	96-97	0.20370146798608246
1106	98-99	0.006394502711806638
1106	100	0.016001357409148653
1107	1	-0.02779882221180685
1107	2	-0.11450795647060374
1107	3	-0.14900218288334344
1107	4	-0.05888366798117062
1107	5	-0.18053935510257446
1107	6	-0.20021045378996405
1107	7	-0.23232733253234983
1107	8	-0.2046419886598727
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2315	100	0.09532163020075757
2316	1	-0.0859100535506272
2316	2	-0.17991403387181748
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2316	4	-0.031825533521470106
2316	5	-0.07900000435247989
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2316	14-15	0.08841602794441172
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2316	20-21	4.1759308150801644E-4
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2316	54-55	0.16964945360268047
2316	56-57	0.09074576953874214
2316	58-59	0.11545890042458495
2316	60-61	-1.131762626016915
2316	62-63	0.24029424123828846
2316	64-65	0.23185004964897615
2316	66-67	0.010345482939428052
2316	68-69	0.18410166943530726
2316	70-71	0.05871636457889906
2316	72-73	0.15953240155022996
2316	74-75	0.10996071055748047
2316	76-77	-0.12857285912956584
2316	78-79	-0.14693973571306884
2316	80-81	-0.056706947865436064
2316	82-83	-0.14746401254669905
2316	84-85	-0.6387902975687183
2316	86-87	-0.10136640352645898
2316	88-89	0.016702205050744823
2316	90-91	0.13118432325462948
2316	92-93	0.30482348813438875
2316	94-95	0.22113483171018444
2316	96-97	0.2945774768838305
2316	98-99	0.32171809957409536
2316	100	0.09111207279463684
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	0.0
10	1.0
11	1.0
12	22.0
13	143.0
14	551.0
15	1535.0
16	3474.0
17	6631.0
18	10852.0
19	15424.0
20	20875.0
21	26276.0
22	33391.0
23	37968.0
24	40977.0
25	44357.0
26	48797.0
27	57864.0
28	67769.0
29	81778.0
30	99612.0
31	122942.0
32	154008.0
33	198895.0
34	284335.0
35	499471.0
36	773079.0
37	566891.0
38	519627.0
39	98864.0
40	78.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.89845829921138	17.30455065446001	12.299082403346171	23.497908642982445
2	17.14879216621839	20.346805419581518	38.69336870638203	23.81103370781806
3	19.494991868188936	26.3263682129117	29.690005981931577	24.488633936967787
4	13.268423642506372	16.08263840888022	35.68252123290507	34.96641671570834
5	13.370356978157883	36.03074036808787	33.03179893514442	17.56710371860982
6	30.511134308679278	34.204526041191755	19.62126476355774	15.663074886571229
7	28.215668153983252	32.07814084815562	21.579497198082116	18.126693799779012
8	27.83371632049344	32.98934361153835	20.404036169359806	18.772903898608405
9	28.512851982173164	15.602177182035968	20.114146678639262	35.7708241571516
10-11	26.125922410967345	24.99667888818347	27.798085834298664	21.07931286655052
12-13	26.169493879176624	23.143407013792814	27.81957458181361	22.867524525216954
14-15	23.723705978125427	24.27710848109383	26.51884983054374	25.480335710237
16-17	22.982420743993732	26.593504924759525	27.191105299979302	23.23296903126744
18-19	22.62874753651293	25.815654460557415	29.157952836513022	22.397645166416634
20-21	23.58819503508747	25.28050849189642	28.903523734809884	22.227772738206223
22-23	23.257311442403594	24.997664724687088	28.851740215720483	22.893283617188832
24-25	22.938945254499476	24.909097269360974	28.97674962781403	23.17520784832552
26-27	22.704568959993544	24.840254973568772	29.17524329136405	23.279932775073632
28-29	22.66939591849142	25.77372769292615	28.95604807410595	22.60082831447648
30-31	23.780115185721122	25.093668978102762	29.001587485436698	22.12462835073942
32-33	23.400202897612697	25.15984115656094	28.935510763884746	22.504445181941616
34-35	23.37953412257043	25.436669193221146	28.780106560765383	22.40369012344304
36-37	23.363936810316307	25.43381493139602	28.684827859536266	22.517420398751405
38-39	23.3291810041684	25.791322662703347	28.34843771453846	22.531058618589785
40-41	23.36015566973584	25.487447280201465	28.562427670159284	22.589969379903412
42-43	23.05624814115557	25.727730224843203	28.057315953110606	23.15870568089062
44-45	22.787425220310205	25.748189958099786	28.367070994520745	23.097313827069264
46-47	22.917836947527523	25.524498752901543	28.05531915172409	23.50234514784685
48-49	22.963411490904402	25.77113768122304	28.065586684957406	23.199864142915157
50-51	22.80438949790076	26.106746716505196	28.007362565466465	23.081501220127578
52-53	22.876051899725173	26.367602307382377	27.316930958690516	23.439414834201934
54-55	22.628795203769066	25.926793542487154	27.87386048900383	23.57055076473995
56-57	23.003424478958543	26.050723374997087	27.323891145118615	23.621961000925754
58-59	22.875967970466206	26.166510704768715	27.65507115505006	23.302450169715023
60-61	23.425059440865446	26.14183583578023	27.729327496765276	22.70377722658905
62-63	23.12278281047466	26.218705841261876	27.68158256532775	22.976928782935722
64-65	22.93876625924526	25.93699827000386	27.465565478159537	23.658669992591346
66-67	22.88035880319107	25.976941230046492	27.34045414533421	23.80224582142823
68-69	22.5128134718515	25.991611142277026	27.56199346467737	23.933581921194104
70-71	22.720376807356296	25.904287347051714	27.60891825621789	23.7664175893741
72-73	22.580818014680595	26.418388960167547	27.032698498335538	23.968094526816316
74-75	22.627866617439242	26.56810875242807	26.86995863271791	23.934065997414773
76-77	22.41655461481348	25.997405611462476	27.302526377877502	24.283513395846544
78-79	22.375539615472796	26.19856539255183	27.256008646949002	24.169886345026367
80-81	22.556131419183593	26.31408702760914	26.815935584791262	24.313845968416008
82-83	22.603988013504132	26.399336138986335	26.92401063131385	24.07266521619568
84-85	22.596138666538103	26.66140352646988	26.523619477059945	24.218838329932073
86-87	22.294085942476357	26.55311185717321	26.717883978521584	24.43491822182884
88-89	21.97446813639563	26.259588008445466	26.94878220436286	24.817161650796045
90-91	22.78836781536914	26.023822999203457	26.91700205424894	24.27080713117847
92-93	22.55009338467569	26.2456923567106	26.812734378766567	24.391479879847147
94-95	22.205218535081624	26.02373776445257	26.824617105889764	24.94642659457604
96-97	21.918626420603797	26.001101030991585	27.130181848290924	24.95009070011369
98-99	22.052251599086226	26.05014075155448	26.80011261696489	25.097495032394406
100	21.846976027954504	26.196447151529668	26.957592152154586	24.998984668361242
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2108.0
1	1625.5
2	3281.0
3	4607.5
4	2744.0
5	1857.0
6	2518.5
7	4475.5
8	6702.0
9	7598.5
10	6490.5
11	5110.5
12	5142.5
13	5507.0
14	6023.5
15	6542.0
16	6882.5
17	7341.5
18	7871.5
19	8297.5
20	8785.0
21	9357.0
22	10273.0
23	11645.0
24	13089.5
25	15172.5
26	18242.5
27	21300.0
28	24062.5
29	27561.0
30	32354.0
31	36547.5
32	41225.0
33	47160.5
34	52832.5
35	58077.5
36	64941.0
37	72243.0
38	77298.0
39	81798.0
40	85625.5
41	89319.0
42	94203.5
43	100550.5
44	107026.5
45	114154.0
46	122869.5
47	134305.0
48	152198.0
49	164196.5
50	170728.5
51	172292.5
52	164105.5
53	159243.5
54	155202.0
55	146781.0
56	135395.0
57	124480.5
58	113968.0
59	100334.0
60	83183.5
61	66657.5
62	54308.5
63	42994.0
64	32460.5
65	24582.0
66	18423.0
67	14247.0
68	11085.0
69	8469.5
70	6616.5
71	5077.0
72	4163.5
73	3373.0
74	2580.5
75	1885.0
76	1335.0
77	851.5
78	553.0
79	367.5
80	231.5
81	145.5
82	94.0
83	57.0
84	38.5
85	30.5
86	17.0
87	14.5
88	13.5
89	9.0
90	5.5
91	3.0
92	0.5
93	0.5
94	1.5
95	1.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04572260449580282
2	0.010611836573524436
3	0.0
4	2.882227217500464E-4
5	3.406268529773276E-4
6	2.6202065613640586E-5
7	0.0
8	7.860619684092176E-5
9	0.0
10-11	0.0
12-13	3.406268529773276E-4
14-15	0.01215775844472923
16-17	0.001650730133659357
18-19	6.550516403410146E-5
20-21	8.122640340228581E-4
22-23	6.943547387614755E-4
24-25	0.00493908936817125
26-27	0.01693963541921864
28-29	0.014437338153115964
30-31	0.010349815917388032
32-33	0.007402083535853465
34-35	0.009445844653717431
36-37	0.00538452448360314
38-39	0.0042185325637961344
40-41	0.005463130680444062
42-43	0.007284174240592083
44-45	0.007415184568660286
46-47	0.016651412697468594
48-49	0.01882618414340076
50-51	0.01843315315919615
52-53	0.0125900925273543
54-55	0.004270936695023416
56-57	0.003825501579591525
58-59	0.010218805589319828
60-61	0.0024891962332958557
62-63	1.5721239368184352E-4
64-65	2.7512168894322617E-4
66-67	7.205568043751161E-4
68-69	5.240413122728117E-5
70-71	1.8341445929548412E-4
72-73	0.0024760952004890353
74-75	0.005030796597818993
76-77	0.004703270777648485
78-79	0.003091843742409589
80-81	7.860619684092176E-5
82-83	8.253650668296785E-4
84-85	3.5372788578414793E-4
86-87	2.6202065613640586E-4
88-89	1.8341445929548412E-4
90-91	3.406268529773276E-4
92-93	5.502433778864523E-4
94-95	1.3101032806820293E-5
96-97	0.001834144592954841
98-99	4.3233408262506964E-4
100	1.5721239368184352E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3816493.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.95166383237375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.42603049899911	42.95637933189367
2	14.992753640397408	17.077245304990218
3	4.337209188281838	7.410338389851296
4	1.7483243033164508	3.982799119697902
5	0.9106190463279399	2.5930634902912804
6	0.5315365129958352	1.816313328166258
7	0.36997693432893586	1.4749561392743642
8	0.27084061773914203	1.2339859050905648
9	0.20419179118853661	1.0466156024189814
>10	1.1083582686751887	11.298108657163727
>50	0.06754453154952564	2.6229819088236215
>100	0.02979998856920892	3.046391827033076
>500	0.0014614672333349312	0.5647820812451412
>1k	0.001082568318100797	1.1567536037041648
>5k	1.082568318100797E-4	0.4054371441188886
>10k+	1.6238524771511955E-4	1.3138481662368486
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16981	0.44493727618523077	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15525	0.40678706865177006	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10114	0.2650076916163609	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7469	0.19570322806828153	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	5683	0.14890633888231944	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	4349	0.1139527833537229	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4018	0.10527989963560788	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.6202065613640586E-5	0.0
2	0.0	0.0	0.0	2.6202065613640586E-5	0.0
3	0.0	0.0	0.0	2.6202065613640586E-5	0.0
4	0.0	0.0	0.0	2.6202065613640586E-5	0.0
5	0.0	0.0	0.0	2.6202065613640586E-5	0.0
6	0.0	0.0	0.0	2.6202065613640586E-5	0.0
7	0.0	0.0	0.0	2.6202065613640586E-5	0.0
8	0.0	0.0	0.0	2.6202065613640586E-5	0.0
9	0.0	0.0	0.0	2.6202065613640586E-5	5.240413122728117E-5
10-11	0.0	0.0	0.0	2.6202065613640586E-5	5.240413122728117E-5
12-13	0.0	0.0	0.0	2.6202065613640586E-5	6.550516403410146E-5
14-15	0.0	0.0	0.0	2.6202065613640586E-5	1.834144592954841E-4
16-17	0.0	0.0	0.0	2.6202065613640586E-5	3.0132375455686674E-4
18-19	0.0	0.0	0.0	9.170722964774205E-5	3.406268529773276E-4
20-21	0.0	0.0	0.0	1.834144592954841E-4	3.5372788578414793E-4
22-23	0.0	0.0	0.0	3.7992995139778845E-4	3.799299513977885E-4
24-25	0.0	0.0	0.0	9.301733292842408E-4	4.323340826250697E-4
26-27	0.0	0.0	0.0	0.0018210435601480207	4.716371810455305E-4
28-29	0.0	0.0	0.0	0.00534522138518268	4.716371810455305E-4
30-31	0.0	0.0	0.0	0.016756220959923154	4.716371810455305E-4
32-33	0.0	0.0	0.0	0.04371814647635932	5.764454435000929E-4
34-35	0.0	0.0	0.0	0.08743629295271863	5.764454435000929E-4
36-37	0.0	0.0	0.0	0.15153964647649032	5.764454435000929E-4
38-39	0.0	0.0	0.0	0.2520900732688361	6.026475091137335E-4
40-41	0.0	0.0	0.0	0.38024437618515217	6.288495747273741E-4
42-43	0.0	0.0	0.0	0.4938434316530909	6.68152673147835E-4
44-45	0.0	0.0	0.0	0.6324654597820565	7.467588699887567E-4
46-47	0.0	0.0	0.0	0.7884463563800589	7.991630012160379E-4
48-49	0.0	0.0	0.0	0.9598209665260751	8.646681652501393E-4
50-51	0.0	0.0	0.0	1.138440447814263	8.646681652501393E-4
52-53	0.0	0.0	0.0	1.3176232735131441	9.563753948978813E-4
54-55	0.0	0.0	0.0	1.5248816125170412	0.0010480826245456234
56-57	0.0	0.0	0.0	1.7578572789207265	0.0010480826245456234
58-59	0.0	0.0	0.0	2.024974236818985	0.0010480826245456234
60-61	0.0	0.0	0.0	2.2977770429554045	0.0011266888213865452
62-63	0.0	0.0	0.0	2.586484502919303	0.0011528908870001857
64-65	0.0	0.0	0.0	2.86452772217845	0.0011528908870001857
66-67	0.0	0.0	0.0	3.186406997209218	0.0011528908870001857
68-69	0.0	0.0	0.0	3.560913121024983	0.0011528908870001857
70-71	0.0	0.0	0.0	3.9629052116694568	0.0011528908870001857
72-73	0.0	0.0	0.0	4.318139716226389	0.0012708001822615686
74-75	0.0	0.0	0.0	4.68038327333497	0.0012839012150683887
76-77	0.0	0.0	0.0	5.090144276433888	0.0012839012150683887
78-79	0.0	0.0	0.0	5.547396523457531	0.0012839012150683887
80-81	0.0	0.0	0.0	6.001203198852979	0.00133630534629567
82-83	0.0	0.0	0.0	6.469761637188906	0.0014411136087502322
84-85	0.0	0.0	0.0	6.949901388526063	0.0014411136087502322
86-87	1.3101032806820293E-5	0.0	0.0	7.446744956691916	0.0014411136087502322
88	2.6202065613640586E-5	0.0	0.0	7.852654256145629	0.0014673156743638728
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	8615	0.0	62.57293	2
ACCTGGG	9610	0.0	55.157833	3
GTACCTG	10235	0.0	53.381428	1
CCTGGGG	8365	0.0	44.71658	4
CTGGGGG	5015	0.0	39.7298	5
TATAACG	495	0.0	38.927547	2
TAACGCA	555	0.0	37.254707	4
ATAACGC	530	0.0	37.23873	3
GTACCCG	925	0.0	35.073513	1
TGGGGGG	7615	0.0	31.163265	6
TACCGTA	345	0.0	29.965742	7
TACCCGG	1080	0.0	27.850601	2
GCGTAAT	70	0.008821288	26.867908	1
GTATAAC	1005	0.0	26.199553	1
GTACACG	810	0.0	26.121576	1
ATGGGGG	7030	0.0	24.999868	5
CATGGGG	10830	0.0	24.515541	4
TATCACG	405	0.0	24.369276	2
GTACATG	19420	0.0	22.662016	1
ACCGTAT	450	0.0	21.935223	8
>>END_MODULE