FastQCFastQC Report
Mon 6 Jun 2016
ERR523124_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523124_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13694467
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT464940.3395093799561531No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT389710.2845747848382854No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226780.16559972724750807No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG190600.045.39472
GTACCTG219400.040.2405241
ACCTGGG227900.036.995813
GGTATCA409300.027.9944231
GTATCAA571200.027.3556731
CCTGGGG218350.025.9265864
GTACATG634100.024.0338421
CATGGGG364400.023.7545854
TATAACG11500.023.7159422
TACATGG650250.023.2131942
TCAACGC668550.023.1736014
CAACGCA675250.022.9565855
ATCAACG681700.022.6875293
TATCAAC683200.022.623622
ACATGGG656100.022.390183
AACGCAG696500.022.3728896
ATGGGGG214400.021.161945
GAGTACT388100.020.1834412-13
ACGCAGA772700.020.0630477
CGCAGAG800650.019.3389038
Produced by FastQC (version 0.11.5)