FastQCFastQC Report
Mon 6 Jun 2016
ERR523124_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523124_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13694467
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT431330.3149666211908795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT392850.2868676816702687No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224230.1637376613489229No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG194500.045.7172932
ACCTGGG226050.038.625653
GTACCTG234850.038.4405441
GGTATCA430400.029.441871
CCTGGGG213350.027.9734274
GTATCAA599600.026.9288311
TCAACGC694050.022.9058194
CAACGCA702950.022.6290975
ATCAACG707900.022.4377543
TATCAAC718200.022.3014532
AACGCAG723400.022.1258096
GTACATG642350.021.3010061
TACATGG660300.020.4561772
GAGTACT400550.020.20642512-13
CATGGGG370250.019.901494
ACGCAGA807450.019.7936367
ACATGGG669650.019.5723
CGCAGAG836000.019.0419648
CTGGGGG131050.018.8975985
ATGGGGG209450.018.6446275
Produced by FastQC (version 0.11.5)