FastQCFastQC Report
Mon 6 Jun 2016
ERR523123_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523123_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5670850
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT335840.5922216246241745No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194280.3425941437350662No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163830.28889848964440956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96140.16953366779230628No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT90910.16031106447887No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC74750.13181445462320465No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC65880.11617306047594275No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG198250.027.7715951
TACATGG203800.026.7843632
ACATGGG205450.025.6552263
CATGGGG147600.024.6852684
ATGGGGG88350.023.4676675
GAGTACT165700.020.95310612-13
AGAGTAC247400.019.8516710-11
GTACTTT182750.018.81920614-15
TACCTGG39150.018.374392
AGTACTT175000.018.2422312-13
GAGTACA149800.018.0473381
AGTACAT149700.016.7087172
GTACCTG47600.016.6930961
TGGGGGG91550.016.3189186
GTATATA29550.016.22931
ACTTTTT226200.015.38022816-17
TACTTTT210400.015.09553814-15
ATTACGC2509.510434E-515.0461833
GTATAGC16100.014.6016261
CATGGGA68300.014.2485994
Produced by FastQC (version 0.11.5)