FastQCFastQC Report
Mon 6 Jun 2016
ERR523123_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523123_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5670850
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT343560.6058351040849256No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181940.32083373744676724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161140.28415493268204944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91860.16198629835033548No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT91190.1608048176199335No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC77990.13752788382693953No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC68440.12068737490852342No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG57090.10067273865469904No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA57080.10065510461394675No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG201200.026.9971661
TACATGG206600.025.957272
ACATGGG206350.025.1658553
CATGGGG144900.023.5787034
ATGGGGG85900.021.6097745
GAGTACT172200.020.9387212-13
AGAGTAC261550.019.17766410-11
GTACTTT189800.018.92011814-15
TACCTGG40750.018.9155922
TATAACG5450.018.1103342
AGTACTT182750.018.08364312-13
GTACCTG48850.016.7513141
GAGTACA155950.016.616151
TATCACG4851.8189894E-1216.4744472
GGGTTAG20250.016.256881
AGTACAT152800.015.6258652
ACTTTTT232600.015.54770316-17
ATAACGC5450.015.5213653
GTATAGC16750.015.1615331
TACTTTT219750.014.97255214-15
Produced by FastQC (version 0.11.5)