FastQCFastQC Report
Mon 6 Jun 2016
ERR523122_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523122_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11024878
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209010.18958032914287126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163600.14839166474223114No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA177550.037.1275181
GTATCAA290400.034.9811821
TCAACGC340500.029.121414
CAACGCA344200.028.7129255
GTACATG391200.028.421261
ATCAACG350850.028.3445033
AACGCAG357100.027.8003376
TATCAAC363450.027.403772
TACATGG403650.026.9801982
ACATGGG411400.025.772083
ACGCAGA409750.024.262487
CGCAGAG432200.022.9460748
CATGGGG290900.022.8914414
GCAGAGT485850.020.2183119
ATGGGGG157250.020.0829545
GAGTACT211350.019.49476612-13
AGAGTAC386750.019.40960910-11
GTACTTT224000.018.17509814-15
GAGTACA298100.017.8832471
CAGAGTA465500.017.23103510-11
Produced by FastQC (version 0.11.5)