FastQCFastQC Report
Mon 6 Jun 2016
ERR523122_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523122_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11024878
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181560.16468209444131718No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153960.13964780381243222No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA174550.038.677311
GTATCAA281150.034.3203541
GTACATG394900.028.2348481
TCAACGC335700.028.1923794
CAACGCA341250.027.7063275
ATCAACG345300.027.4228123
TACATGG408350.026.6971632
AACGCAG355700.026.6465956
TATCAAC359550.026.4488092
ACATGGG413200.025.39583
CATGGGG295150.023.1337724
ACGCAGA410500.023.020817
CGCAGAG431150.021.9122928
ATGGGGG161700.019.8488035
GCAGAGT485250.019.275599
AGAGTAC394250.019.09927410-11
GAGTACA305700.018.990831
GAGTACT215350.018.91253512-13
AGTACAT293950.018.5276052
AGTACTT235150.017.54993812-13
Produced by FastQC (version 0.11.5)