##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR523122_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11024878
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55947140639561	34.0	31.0	34.0	30.0	35.0
2	32.649608458252324	34.0	31.0	34.0	30.0	34.0
3	32.905467797466784	34.0	31.0	34.0	31.0	36.0
4	35.69956184549162	37.0	35.0	37.0	32.0	37.0
5	35.77273036490744	37.0	35.0	37.0	35.0	37.0
6	35.85066474204975	37.0	35.0	37.0	35.0	37.0
7	35.678655763809815	37.0	35.0	37.0	35.0	37.0
8	35.71851498039253	37.0	35.0	37.0	33.0	37.0
9	36.93039034082735	39.0	35.0	39.0	34.0	39.0
10-11	37.03618969751865	38.0	36.0	39.0	33.5	39.0
12-13	36.902638242346086	38.0	36.0	39.0	33.5	39.0
14-15	37.9853826500393	39.0	36.0	41.0	33.5	41.0
16-17	38.00450490245788	39.0	37.0	41.0	34.0	41.0
18-19	37.992017281279665	39.0	37.0	41.0	33.5	41.0
20-21	37.87025606995379	39.0	36.5	41.0	34.0	41.0
22-23	37.7801818759355	39.0	36.5	41.0	34.0	41.0
24-25	37.63326591913307	39.0	36.0	41.0	33.0	41.0
26-27	37.501792808954434	38.0	36.5	41.0	33.0	41.0
28-29	37.472084906517786	38.0	36.5	41.0	33.5	41.0
30-31	37.32546174207098	38.0	36.0	41.0	33.5	41.0
32-33	37.18693966500128	38.0	36.5	40.0	32.5	41.0
34-35	37.01200230968543	38.0	36.0	40.0	32.0	41.0
36-37	37.03502070499102	38.0	35.5	40.0	33.0	41.0
38-39	37.17265188784856	38.0	36.0	40.0	32.0	41.0
40-41	37.089194773856	38.0	36.5	40.0	32.5	41.0
42-43	37.12799320772529	38.0	36.5	40.0	32.5	41.0
44-45	36.9620749998322	38.0	35.5	40.0	32.0	41.0
46-47	36.72635484038916	38.0	35.5	40.0	31.5	41.0
48-49	36.692062352073194	38.0	36.0	40.0	31.0	41.0
50-51	36.30579839522941	37.0	35.0	40.0	30.5	41.0
52-53	36.33883096030632	37.5	35.0	40.0	30.5	41.0
54-55	36.134788294255955	37.5	35.0	40.0	31.0	41.0
56-57	35.81885096596987	37.0	35.0	40.0	29.0	41.0
58-59	35.681478561486124	36.5	35.0	40.0	29.0	41.0
60-61	35.39541879737807	36.5	35.0	39.5	29.0	41.0
62-63	35.190450270742225	36.5	34.5	39.0	28.5	41.0
64-65	35.048010281837136	37.0	34.0	39.0	28.0	41.0
66-67	34.81095967683271	36.5	34.0	39.0	28.0	40.0
68-69	34.34467751026361	36.0	34.0	37.5	27.0	40.0
70-71	34.092787512025076	36.0	33.5	37.5	26.0	39.5
72-73	33.84359532141762	35.5	33.0	37.0	26.0	39.0
74-75	33.558321008178055	35.0	33.0	37.0	26.0	39.0
76-77	32.10014092672953	34.0	30.5	36.0	24.5	37.5
78-79	33.14389020903451	35.0	32.5	37.0	26.0	37.0
80-81	33.165736029006396	35.0	33.0	36.5	26.0	37.0
82-83	32.9848936196845	35.0	33.0	36.0	26.0	37.5
84-85	32.78639922364674	35.0	33.0	36.0	25.0	37.0
86-87	32.54812003361852	35.0	32.5	36.0	25.0	37.0
88-89	32.38591533620599	35.0	32.0	36.0	25.0	36.5
90-91	32.242402727721796	35.0	32.0	35.5	24.0	36.5
92-93	32.08682581340129	35.0	32.0	35.0	24.0	36.5
94-95	31.963073786394734	35.0	32.0	35.0	24.0	36.0
96-97	31.770465396533186	35.0	31.0	35.0	23.0	36.5
98-99	31.624749543713772	34.5	31.0	35.0	22.0	36.5
100	30.39382612669274	34.0	29.0	35.0	19.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.09302338147237776
1101	2	-0.09356875639085871
1101	3	-0.144582838692898
1101	4	-0.12940613550343727
1101	5	-0.09571870647614134
1101	6	-0.194630675859095
1101	7	-0.142277209653642
1101	8	-0.13447175564323288
1101	9	-0.19107029593710934
1101	10-11	-0.10335813600661226
1101	12-13	-0.13015042737808358
1101	14-15	-0.29194299779369715
1101	16-17	-0.1389779603325394
1101	18-19	-0.1594811371506708
1101	20-21	-0.19426606329412976
1101	22-23	-0.12781835467924196
1101	24-25	-0.2533206247076407
1101	26-27	-0.17693213640711036
1101	28-29	-0.2514399683771771
1101	30-31	-0.256417388456633
1101	32-33	-0.1578105032967443
1101	34-35	-0.14811054688262004
1101	36-37	-0.36954829592118443
1101	38-39	-0.3064760910279034
1101	40-41	-0.30549161874290576
1101	42-43	-0.289738697731444
1101	44-45	-0.3628805940728981
1101	46-47	-0.3255194989878234
1101	48-49	-0.36756068566747047
1101	50-51	-0.2794152408260473
1101	52-53	-0.40747421290316055
1101	54-55	-0.3676649811799635
1101	56-57	-0.432269152482732
1101	58-59	-0.5462609923188211
1101	60-61	-0.5378457441689193
1101	62-63	-0.3126714673370401
1101	64-65	-0.4000544911487154
1101	66-67	-0.4984093178072655
1101	68-69	-0.4465560285277945
1101	70-71	-0.5255731240962831
1101	72-73	-0.5832968268301642
1101	74-75	-0.5975936377184468
1101	76-77	-0.5150031694038475
1101	78-79	-0.4631839025504405
1101	80-81	-0.3657418158106367
1101	82-83	-0.5172642374606937
1101	84-85	-0.514418096017927
1101	86-87	-0.5514287094377721
1101	88-89	-0.5634841008918912
1101	90-91	-0.6214368105457311
1101	92-93	-0.8622858441004588
1101	94-95	-0.7815275922402343
1101	96-97	-0.6504998408013982
1101	98-99	-0.6979183027472864
1101	100	-0.6180952119013874
1102	1	-0.05415540302361421
1102	2	-0.11607797623852179
1102	3	-0.10827950050045132
1102	4	-0.08265506022052449
1102	5	-0.09482175844749463
1102	6	-0.14854582331336985
1102	7	-0.13252140636964072
1102	8	-0.08549132143843252
1102	9	-0.15690156437360514
1102	10-11	-0.14025543966759102
1102	12-13	-0.18189346151111607
1102	14-15	-0.21564989526957135
1102	16-17	-0.12812180282020336
1102	18-19	-0.16447045918970815
1102	20-21	-0.23948348280465126
1102	22-23	-0.15652855880265548
1102	24-25	-0.21615629421524574
1102	26-27	-0.1814553739919802
1102	28-29	-0.14549354789403424
1102	30-31	-0.1889798386900594
1102	32-33	-0.2125296326020134
1102	34-35	-0.21618680145311941
1102	36-37	-0.294457734861048
1102	38-39	-0.31509726153964124
1102	40-41	-0.3511931740568386
1102	42-43	-0.3391221231208945
1102	44-45	-0.45684504245927826
1102	46-47	-0.34869692134230235
1102	48-49	-0.33893822251314276
1102	50-51	-0.284023055206724
1102	52-53	-0.339778385330753
1102	54-55	-0.43818658840294233
1102	56-57	-0.3151971356220855
1102	58-59	-0.4563825636604122
1102	60-61	-0.4003603572894292
1102	62-63	-0.3461636191152735
1102	64-65	-0.36089358460304055
1102	66-67	-0.4420318331791009
1102	68-69	-0.4357499444899844
1102	70-71	-0.38747634285934396
1102	72-73	-0.5511753845453384
1102	74-75	-0.8588811602316326
1102	76-77	-0.4663470370250735
1102	78-79	-0.3943246793518682
1102	80-81	-0.3779294678163154
1102	82-83	-0.3922564221255769
1102	84-85	-0.3563702771638333
1102	86-87	-0.4472982045456817
1102	88-89	-0.4357525721079085
1102	90-91	-0.3524453041551041
1102	92-93	-0.5127526149900348
1102	94-95	-0.5948724394756759
1102	96-97	-0.6431295129453254
1102	98-99	-0.8216937936212219
1102	100	-0.649847310439899
1103	1	-0.15563976504449784
1103	2	-0.1199409343212281
1103	3	-0.16202016319115842
1103	4	-0.051826053222718826
1103	5	-0.14367432091175658
1103	6	-0.12098412850193085
1103	7	-0.10236184421162875
1103	8	-0.10041265159466661
1103	9	-0.11983496524828041
1103	10-11	-0.08186746705889192
1103	12-13	-0.10240473004863304
1103	14-15	-0.2138836221349223
1103	16-17	-0.1757562746127661
1103	18-19	-0.13745972705487475
1103	20-21	-0.16947868927419307
1103	22-23	-0.114217941090196
1103	24-25	-0.1741548031024891
1103	26-27	-0.22018781847315694
1103	28-29	-0.21406889074879132
1103	30-31	-0.2066528874349487
1103	32-33	-0.13365279045540746
1103	34-35	-0.08705902164332002
1103	36-37	-0.1360774439741803
1103	38-39	-0.23907806242142016
1103	40-41	-0.33504960416389196
1103	42-43	-0.37281736035134116
1103	44-45	-0.39716824102111303
1103	46-47	-0.3636146168155534
1103	48-49	-0.3485510067687372
1103	50-51	-0.16657385617181575
1103	52-53	-0.2352897516433714
1103	54-55	-0.28453757923877987
1103	56-57	-0.15229580923060837
1103	58-59	-0.23800490930031515
1103	60-61	-0.34667058866271816
1103	62-63	-0.2866187375396265
1103	64-65	-0.39290484982470986
1103	66-67	-0.39106059062084597
1103	68-69	-0.28840909556747363
1103	70-71	-0.3701417022304412
1103	72-73	-0.38840975723645954
1103	74-75	-0.40739954441566795
1103	76-77	-0.3029313753588809
1103	78-79	-0.33860161379817555
1103	80-81	-0.3437875259212504
1103	82-83	-0.3166599770240808
1103	84-85	-0.2630379649426118
1103	86-87	-0.4171344423095533
1103	88-89	-0.36788105691867656
1103	90-91	-0.3877446141349843
1103	92-93	-0.48808037412253213
1103	94-95	-0.1779623893349047
1103	96-97	-0.9191637821818937
1103	98-99	-1.258964555653126
1103	100	-0.5035148968726624
1104	1	-0.053755039547858985
1104	2	-0.08523740412987735
1104	3	-0.08570819900095472
1104	4	-0.12815238499164394
1104	5	-0.1281249102085127
1104	6	-0.09494585106941145
1104	7	-0.15381629442529743
1104	8	-0.062204961011161686
1104	9	-0.10159419799985159
1104	10-11	-0.11219653803794216
1104	12-13	-0.10322628348801288
1104	14-15	-0.19750639888979293
1104	16-17	-0.02981901572486123
1104	18-19	-0.12256018626668919
1104	20-21	-0.21832618835794193
1104	22-23	-0.09466122116896258
1104	24-25	-0.12631593188127965
1104	26-27	-0.1237019983609926
1104	28-29	-0.1995251776782183
1104	30-31	-0.2853459284718909
1104	32-33	-0.189489956307483
1104	34-35	-0.11076056610050244
1104	36-37	-0.16171278397660416
1104	38-39	-0.19207304091977306
1104	40-41	-0.1450352809701343
1104	42-43	-0.15737045837693842
1104	44-45	-0.25810609047568533
1104	46-47	-0.07845155013971095
1104	48-49	-0.1597090097772167
1104	50-51	-0.05974664249822581
1104	52-53	-0.06940520565927244
1104	54-55	-0.21618697974169976
1104	56-57	-0.09857779137453804
1104	58-59	-0.10565139058741124
1104	60-61	-0.20169935217722212
1104	62-63	-0.0913371802584777
1104	64-65	-0.25032087531351976
1104	66-67	-0.3169247063682974
1104	68-69	-0.18996564353804501
1104	70-71	-0.2256333352117892
1104	72-73	-0.15302796307931743
1104	74-75	-0.2698027101817644
1104	76-77	-0.27931766382288004
1104	78-79	-0.292200289515101
1104	80-81	-0.10759078264366195
1104	82-83	-0.11567017989545292
1104	84-85	-0.15755173213243978
1104	86-87	-0.25436290185277954
1104	88-89	-0.25027718109171104
1104	90-91	-0.21534193824741976
1104	92-93	-0.2791678601011931
1104	94-95	-0.3481926018378054
1104	96-97	-0.1066659531411247
1104	98-99	-0.9769249044236332
1104	100	-0.30304545185490994
1105	1	-0.15492054048954884
1105	2	-1.0575830630281722
1105	3	-0.1979825952182992
1105	4	-0.10501487465930381
1105	5	-0.14250040071179626
1105	6	-0.13650788517458068
1105	7	-0.1485388768012541
1105	8	-0.1602787921005202
1105	9	-0.19335033769097976
1105	10-11	-0.12385158883972025
1105	12-13	-0.12400565756306747
1105	14-15	-0.12411427835837685
1105	16-17	-0.6252461631465565
1105	18-19	-0.11531687361148357
1105	20-21	-0.1813113366522643
1105	22-23	-0.20978862509546303
1105	24-25	-0.21555694612268184
1105	26-27	-0.20062004798295163
1105	28-29	-0.2674742088569957
1105	30-31	-0.18743682139864148
1105	32-33	-0.16385112650279154
1105	34-35	-0.14067249489038858
1105	36-37	-0.1997915112217541
1105	38-39	-0.2716007596225509
1105	40-41	-0.27484526735005943
1105	42-43	-0.2658945576788696
1105	44-45	-0.36403487709234383
1105	46-47	-0.2923036070408003
1105	48-49	-0.25575769104398915
1105	50-51	-0.16298985198934446
1105	52-53	-0.28636205967895734
1105	54-55	-0.35604755625514173
1105	56-57	-0.20555424309499415
1105	58-59	-0.2801729658785632
1105	60-61	-0.26002337406303866
1105	62-63	-0.13230800731780334
1105	64-65	-0.18981208233784486
1105	66-67	-0.21304217583461593
1105	68-69	-0.230137611057188
1105	70-71	-0.2166937248247507
1105	72-73	-0.3275037295084644
1105	74-75	-0.3151275808041163
1105	76-77	-0.2309639951732514
1105	78-79	-0.15596272675149692
1105	80-81	-0.10236537332829698
1105	82-83	-0.08836532949637643
1105	84-85	-0.1339251566390729
1105	86-87	-0.21024123291935837
1105	88-89	-0.15705008403698884
1105	90-91	-0.14841316714180408
1105	92-93	-0.20845776173890584
1105	94-95	-0.1979288985651415
1105	96-97	-0.17847659399721394
1105	98-99	-0.19875004983294176
1105	100	-0.16447080115565882
1106	1	-0.09973746492838131
1106	2	-0.11481380500261906
1106	3	-0.06951320026350771
1106	4	-0.0879831138528715
1106	5	-0.1068446145783497
1106	6	-0.05613923377426744
1106	7	-0.05204194270373108
1106	8	-0.027582193917346842
1106	9	-0.145789973872553
1106	10-11	-0.13300824366666575
1106	12-13	-0.06440429797684288
1106	14-15	-0.08358864199828986
1106	16-17	-0.0221263198258157
1106	18-19	-0.0716939619518584
1106	20-21	-0.11568262062072421
1106	22-23	-0.0011745715776001475
1106	24-25	-0.04197991015183078
1106	26-27	-0.06627533728740076
1106	28-29	-0.08170859355271176
1106	30-31	-0.015378068850331772
1106	32-33	0.10896700892791955
1106	34-35	0.10233473978275498
1106	36-37	-0.0343879255650279
1106	38-39	-0.02427733121796649
1106	40-41	-0.07759624723744452
1106	42-43	-0.13470498277781218
1106	44-45	-0.2011813346110074
1106	46-47	-0.1392761133337217
1106	48-49	-0.1428719682666113
1106	50-51	-0.025784419485212595
1106	52-53	-0.1135359096292845
1106	54-55	-0.10288113974247892
1106	56-57	-0.11034610121011212
1106	58-59	-0.22158936494952286
1106	60-61	-0.11126885671365017
1106	62-63	-0.11276297987647865
1106	64-65	-0.09288678961616625
1106	66-67	-0.04162698393630393
1106	68-69	-0.06288941602971221
1106	70-71	-0.14243329216471778
1106	72-73	-0.19581768777278796
1106	74-75	-0.10864695349708597
1106	76-77	-0.1572141909498903
1106	78-79	-0.11620431503973805
1106	80-81	0.01711360614607571
1106	82-83	-9.752315124060829E-4
1106	84-85	0.10810851213559403
1106	86-87	0.04742838784405734
1106	88-89	0.008962290001299777
1106	90-91	-0.012823700601487076
1106	92-93	-0.050770607093092224
1106	94-95	-0.057105656199805566
1106	96-97	-0.08898496118696997
1106	98-99	-0.016780657528560283
1106	100	-0.04677317918866564
1107	1	-0.03464351283917466
1107	2	-0.05264646532289419
1107	3	-0.022346343984125383
1107	4	-0.0864054980659077
1107	5	-0.02123293927795089
1107	6	-0.07974419476175143
1107	7	-0.07527841779299393
1107	8	-0.053165338113785765
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2316	82-83	-0.08394548643922661
2316	84-85	0.026305573320676956
2316	86-87	-0.09460201931417345
2316	88-89	-0.047480549789774784
2316	90-91	-0.04184944848672956
2316	92-93	-0.17055948623126937
2316	94-95	-0.09464464398012495
2316	96-97	0.07192976647338156
2316	98-99	0.17238234961353172
2316	100	-0.04443334056888304
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	38.0
13	212.0
14	891.0
15	2236.0
16	4787.0
17	8645.0
18	14216.0
19	21719.0
20	30733.0
21	43437.0
22	57102.0
23	67480.0
24	75821.0
25	86783.0
26	103495.0
27	125731.0
28	155843.0
29	192558.0
30	240609.0
31	301943.0
32	388757.0
33	524509.0
34	796775.0
35	1518529.0
36	2332182.0
37	1867206.0
38	1758857.0
39	303698.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.44760839493815	18.72314476472278	13.199025476714166	24.630221363624898
2	16.290000780635875	20.33747070346185	38.83402047444507	24.53850804145721
3	19.170708389155518	24.918241857520325	29.426899322333078	26.484150430991082
4	13.486209415306854	16.380518349547117	36.73687243857687	33.396399796569156
5	14.210901835803059	37.306607193004226	34.20831310604758	14.274177865145141
6	32.46508980173495	36.03288662175514	17.266945837593052	14.23507773891686
7	28.4222853461313	31.72977802836258	21.67288578366906	18.175050841837056
8	26.458803880728578	34.84267386447527	19.936331031470125	18.76219122332602
9	26.930948980201773	15.35456586046266	19.62720309714113	38.08728206219443
10-11	25.620140817923104	25.41228812118051	27.914810825519567	21.05276023537682
12-13	26.00615916503694	23.03279104127386	27.848534463577852	23.112515330111346
14-15	23.38607273419995	24.458131117501384	25.16781570616036	26.987980442138305
16-17	23.373570922049502	27.418885806737304	25.80700579041768	23.40053748079551
18-19	23.367264536855217	26.654885410305095	27.298866552192013	22.67898350064767
20-21	24.00443870045017	25.83033227168609	27.19076819967186	22.97446082819188
22-23	23.889021344466798	25.594747339385187	27.04672402383729	23.469507292310723
24-25	23.67552447831539	25.864110681125542	27.0522468526267	23.408117987932375
26-27	23.544482543433066	25.984282288726558	27.096000607035858	23.375234560804522
28-29	23.43607023602729	25.971052885928298	27.193993686774597	23.39888319126981
30-31	23.73617492714491	25.87496297204771	27.020197679259628	23.368664421547752
32-33	23.564456066506278	26.033368615888364	26.919015628053742	23.483159689551613
34-35	23.789523162949695	25.964261746359018	27.01347478555646	23.232740305134822
36-37	23.826757355339918	25.86924806678678	26.942012969074714	23.361981608798583
38-39	23.698297622665628	26.0368004926458	26.903429694454296	23.36147219023428
40-41	23.70994850882962	25.878507179206988	27.050037545881285	23.36150676608211
42-43	23.608001928761364	25.926270774730032	26.852378279592752	23.613349016915848
44-45	23.546705689649816	25.981328926693347	26.860221388758305	23.61174399489853
46-47	23.578431103501295	25.971158861499294	26.73559468308727	23.71481535191214
48-49	23.644804028427647	26.0850324573161	26.619240385085124	23.65092312917113
50-51	23.49623468448286	26.13328907588136	26.592608310029075	23.777867929606703
52-53	23.641865571074746	26.112199538899294	26.50123965262876	23.7446952373972
54-55	23.47600227633197	26.014430798183795	26.652803318786074	23.856763606698163
56-57	23.464046504322898	26.11077970607485	26.46100072750876	23.96417306209349
58-59	23.52299212619852	25.98565381669159	26.61568945991826	23.87566459719163
60-61	23.573378512673763	26.09979964638785	26.621909113767256	23.704912727171134
62-63	23.373648188647962	26.068914514912606	26.656890573901514	23.900546722537914
64-65	23.299265442279832	25.91591585871304	26.68693795984691	24.097880739160217
66-67	23.286617734203645	26.014380770683115	26.52003254907691	24.178968946036324
68-69	23.19047484494106	26.05939461169421	26.571159071324956	24.178971472039773
70-71	23.246851731643403	25.848492636689517	26.582531132791566	24.322124498875517
72-73	23.240514136952008	26.06029861306387	26.35014468038787	24.349042569596254
74-75	23.112876302986958	26.219639605079642	26.353370003779464	24.314114088153936
76-77	23.16940546003737	25.969311702131424	26.41075215229999	24.450530685531213
78-79	23.062058467153427	25.997688939911168	26.38855906428462	24.551693528650777
80-81	23.10469672639649	26.205472306918598	26.185127337692975	24.504703628991937
82-83	23.173488811612145	26.05995754636801	26.261495288684518	24.505058353335325
84-85	23.036770549999265	26.07358191711178	26.168177844697688	24.72146968819127
86-87	22.917416211353935	26.127865864695238	26.313194355408655	24.641523568542176
88-89	22.884931473797934	26.01156614642258	26.299957885708363	24.80354449407112
90-91	23.01322771059448	25.954585457432344	26.236391725997066	24.795795105976108
92-93	23.002488380332345	25.943863866711197	26.178279924555838	24.875367828400623
94-95	22.85151811303208	25.773845959667767	26.29309103498939	25.081544892310763
96-97	22.844196178541193	25.736475003831217	26.326516719330144	25.09281209829744
98-99	22.72354075200924	25.712005858221943	26.266847070827804	25.297606318941018
100	22.758146190356328	25.64978808226354	26.265726019506864	25.32633970787327
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	787.0
1	573.5
2	555.0
3	728.0
4	609.5
5	634.0
6	991.5
7	2017.0
8	3335.5
9	4017.5
10	3800.0
11	3475.0
12	3822.5
13	4337.5
14	4812.5
15	5418.5
16	6043.0
17	6697.0
18	7431.0
19	8177.0
20	9239.5
21	10652.0
22	12455.0
23	15331.0
24	19081.5
25	24086.5
26	31921.5
27	41433.5
28	51530.5
29	65315.0
30	83033.0
31	100354.5
32	120669.5
33	144349.5
34	168009.5
35	191265.0
36	220518.5
37	248062.5
38	267451.5
39	286401.5
40	299074.5
41	309461.5
42	322790.5
43	342659.0
44	363410.5
45	381889.0
46	397163.0
47	413090.5
48	435889.5
49	448633.0
50	446635.0
51	441495.5
52	435664.5
53	432058.5
54	428222.5
55	411872.0
56	382258.0
57	347667.5
58	315156.5
59	279632.5
60	241055.0
61	204202.0
62	169723.5
63	134191.0
64	102059.0
65	79184.0
66	60624.5
67	47497.0
68	37826.0
69	29319.0
70	23342.0
71	18418.5
72	14608.0
73	12297.5
74	10240.5
75	8028.0
76	5866.0
77	4038.0
78	2747.5
79	1898.0
80	1347.0
81	911.0
82	576.5
83	363.0
84	249.5
85	174.0
86	118.0
87	76.0
88	49.5
89	31.0
90	21.5
91	14.0
92	9.5
93	8.0
94	4.5
95	3.0
96	2.0
97	0.5
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029787177690310952
2	0.07454050738702052
3	0.0026122738047532133
4	9.070395155393102E-6
5	0.001505685595795255
6	8.254059591407723E-4
7	9.070395155393102E-6
8	3.718862013711172E-4
9	9.070395155393102E-6
10-11	2.7211185466179304E-5
12-13	6.349276608775172E-5
14-15	0.010684925493053075
16-17	0.0010158842574040274
18-19	1.9047829826325516E-4
20-21	2.675766570840965E-4
22-23	0.001972810946298
24-25	8.118003664076826E-4
26-27	0.007415048039533862
28-29	0.007959271748857447
30-31	0.010081744215219434
32-33	0.009220056675457089
34-35	0.004285761710923241
36-37	0.0038231715579981926
38-39	0.002943343227925062
40-41	0.003324299824451572
42-43	0.0016870934989031171
44-45	0.005492124266590524
46-47	0.017936706419789863
48-49	0.018290451830850193
50-51	0.01359652233793426
52-53	0.007455864817733131
54-55	0.0011247289992687448
56-57	0.002435401099223048
58-59	0.003111145538299834
60-61	0.003356046207495448
62-63	0.003306159034140786
64-65	0.0038186363604204962
66-67	0.002675766570840965
68-69	0.006925246701142634
70-71	4.3084376988117236E-4
72-73	0.005242688399817213
74-75	0.0075511039668647575
76-77	0.00672116281014629
78-79	0.0045351975776965515
80-81	3.5828060863802756E-4
82-83	0.0013061369023766067
84-85	8.208707615630759E-4
86-87	0.0011746161726234067
88-89	0.001061236233180993
90-91	9.569266888939723E-4
92-93	3.2653422559415166E-4
94-95	0.001111123406535655
96-97	0.0028889208569927035
98-99	0.0033787221953839306
100	0.005015928520932385
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.1024878E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.07681443639871
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.1542780267086	32.76640577736794
2	16.07187098026462	15.453687175278471
3	6.410565762922336	9.245987418490436
4	3.140649634134535	6.039697186801182
5	1.7952031024524286	4.315382321612633
6	1.0724599160112047	3.0936273823527864
7	0.7043004777632139	2.37023663638223
8	0.5077260918289659	1.95278824811033
9	0.354989499291384	1.5360087855871765
>10	1.6528663942767494	13.691520320171268
>50	0.08259096307649372	2.7185433082717254
>100	0.048255970276338406	4.384434014673939
>500	0.0029016446821589882	0.9204015945816151
>1k	0.001247940755252494	0.8695067539043178
>5k	3.1198518602014436E-5	0.1385181097221333
>10k+	6.239703720402887E-5	0.5032549666916099
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18156	0.16468209444131718	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15396	0.13964780381243222	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.5351975776965515E-5
2	0.0	0.0	0.0	0.0	4.5351975776965515E-5
3	0.0	0.0	0.0	0.0	4.5351975776965515E-5
4	0.0	0.0	0.0	0.0	6.349276608775172E-5
5	0.0	0.0	0.0	0.0	7.256316124314482E-5
6	0.0	0.0	0.0	0.0	1.1791513702011033E-4
7	0.0	0.0	0.0	0.0	1.1791513702011033E-4
8	0.0	0.0	0.0	0.0	1.1791513702011033E-4
9	9.070395155393102E-6	0.0	0.0	9.070395155393102E-6	1.5419671764168276E-4
10-11	9.070395155393102E-6	0.0	0.0	9.070395155393102E-6	1.587319152193793E-4
12-13	9.070395155393102E-6	0.0	0.0	1.8140790310786204E-5	2.585062619287034E-4
14-15	9.070395155393102E-6	0.0	0.0	1.8140790310786204E-5	3.764213989488138E-4
16-17	9.070395155393102E-6	0.0	0.0	1.8140790310786204E-5	5.306181165904964E-4
18-19	9.070395155393102E-6	0.0	0.0	2.2675987888482757E-5	5.941108826782482E-4
20-21	9.070395155393102E-6	0.0	0.0	5.8957568510055165E-5	6.757444390767863E-4
22-23	9.070395155393102E-6	0.0	0.0	1.678023103747724E-4	8.254059591407724E-4
24-25	9.070395155393102E-6	0.0	0.0	3.628158062157241E-4	9.750674792047586E-4
26-27	9.070395155393102E-6	0.0	0.0	9.569266888939723E-4	0.0010294898501371171
28-29	9.070395155393102E-6	0.0	0.0	0.0034376797638939857	0.0010430954428702068
30-31	9.070395155393102E-6	0.0	0.0	0.013220100938985447	0.0010793770234917791
32-33	9.070395155393102E-6	0.0	0.0	0.0405038495664079	0.001161010579890317
34-35	9.070395155393102E-6	0.0	0.0	0.08367439530850138	0.0012154329508226758
36-37	9.070395155393102E-6	0.0	0.0	0.15340759326316356	0.001233573741133462
38-39	1.3605592733089654E-5	0.0	0.0	0.255059511769654	0.0012698553217550344
40-41	1.8140790310786204E-5	0.0	0.0	0.3657228678630276	0.0013106720999543034
42-43	1.8140790310786204E-5	0.0	0.0	0.4798601853009167	0.0013424184829981794
44-45	1.8140790310786204E-5	0.0	0.0	0.624678114351923	0.0013968408539305377
46-47	1.8140790310786204E-5	0.0	0.0	0.7968977071673718	0.0014421928297075034
48-49	1.8140790310786204E-5	0.0	0.0	0.979276142556861	0.001537431978839131
50-51	1.8140790310786204E-5	0.0	0.0	1.17163654781486	0.0015601079667276136
52-53	1.8140790310786204E-5	0.0	0.0	1.366214664688353	0.001718839881946993
54-55	1.8140790310786204E-5	0.0	0.0	1.583087812853802	0.001850360611700193
56-57	1.8140790310786204E-5	0.0	0.0	1.8375577489383557	0.0018957125874771584
58-59	1.8140790310786204E-5	0.0	0.0	2.116749047018933	0.001954670155987214
60-61	1.8140790310786204E-5	0.0	0.0	2.410797652364044	0.001995486934186483
62-63	1.8140790310786204E-5	0.0	0.0	2.711190999120353	0.0020589797002742343
64-65	1.8140790310786204E-5	0.0	0.0	3.0256253175772105	0.002113402071206593
66-67	1.8140790310786204E-5	0.0	0.0	3.3759647952566914	0.0021587540469835582
68-69	1.8140790310786204E-5	0.0	0.0	3.7651437049915657	0.0021905004300274344
70-71	1.8140790310786204E-5	0.0	0.0	4.183397766397052	0.00222224681307131
72-73	1.8140790310786204E-5	0.0	0.0	4.60509404276401	0.002517034655621586
74-75	1.8140790310786204E-5	0.0	0.0	5.033810804981243	0.002598668212020124
76-77	1.8140790310786204E-5	9.070395155393102E-6	0.0	5.490786383305103	0.0026032034095978206
78-79	1.8140790310786204E-5	1.3605592733089654E-5	0.0	5.993581062756432	0.0026122738047532137
80-81	1.8140790310786204E-5	1.8140790310786204E-5	0.0	6.528008745312193	0.002716583349040234
82-83	1.8140790310786204E-5	1.8140790310786204E-5	0.0	7.072676903998394	0.002993230401279724
84-85	1.8140790310786204E-5	1.8140790310786204E-5	0.0	7.613989016477099	0.003002300796435117
86-87	1.8140790310786204E-5	1.8140790310786204E-5	0.0	8.173088173855529	0.003034047179478993
88	1.8140790310786204E-5	1.8140790310786204E-5	0.0	8.610362853901876	0.003093004747989048
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17455	0.0	38.67731	1
GTATCAA	28115	0.0	34.320354	1
GTACATG	39490	0.0	28.234848	1
TCAACGC	33570	0.0	28.192379	4
CAACGCA	34125	0.0	27.706327	5
ATCAACG	34530	0.0	27.422812	3
TACATGG	40835	0.0	26.697163	2
AACGCAG	35570	0.0	26.646595	6
TATCAAC	35955	0.0	26.448809	2
ACATGGG	41320	0.0	25.3958	3
CATGGGG	29515	0.0	23.133772	4
ACGCAGA	41050	0.0	23.02081	7
CGCAGAG	43115	0.0	21.912292	8
ATGGGGG	16170	0.0	19.848803	5
GCAGAGT	48525	0.0	19.27559	9
AGAGTAC	39425	0.0	19.099274	10-11
GAGTACA	30570	0.0	18.99083	1
GAGTACT	21535	0.0	18.912535	12-13
AGTACAT	29395	0.0	18.527605	2
AGTACTT	23515	0.0	17.549938	12-13
>>END_MODULE