FastQCFastQC Report
Mon 6 Jun 2016
ERR523121_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523121_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7799544
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT633970.8128295705492526No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249130.319416109454604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203080.26037419623506197No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT187660.24060380965861594No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC141920.1819593555725822No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107580.13793114058975756No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG105930.1358156322985036No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA93990.12050704502724774No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG286050.026.04921
TACATGG293150.025.1286662
ACATGGG305900.023.268153
CATGGGG217700.022.0690064
GAGTACT219550.020.69479612-13
TATAACG5000.020.6844042
ATGGGGG126300.019.3924675
TACCTGG53500.019.155482
AGAGTAC360950.018.92570110-11
GTACTTT238300.018.72107114-15
AGTACTT232300.017.69811212-13
GTATATG26250.017.3719881
GAGTACA230000.016.6380961
GTACCTG69050.015.8635111
AGTACAT225250.015.569132
ACTTTTT296300.015.47548816-17
TAACGCA7300.015.4553454
GTATAGG19100.015.2603841
TACTTTT268200.015.15364414-15
GTATTAG19450.014.2606561
Produced by FastQC (version 0.11.5)