FastQCFastQC Report
Mon 6 Jun 2016
ERR523121_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523121_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7799544
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT654280.838869554425233No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235150.301491984659616No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200120.25657910257317607No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT188730.2419756847323382No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC154010.19746026177940662No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC136170.17458712970912146No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA112320.14400841895372346No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG109830.14081592462328568No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105610.13540535190262404No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA103270.13240517650775482No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG282300.025.8214911
TACATGG291550.024.7787272
ACATGGG299500.023.3330273
CATGGGG210350.023.0118544
GAGTACT215350.021.08820212-13
ATGGGGG120200.020.7999045
GTACTTT232550.019.30305714-15
AGAGTAC355100.018.74642610-11
AGTACTT224950.018.16105512-13
TACCTGG54850.017.566912
TATACCG6800.016.5865655
ACTTTTT285300.015.81369416-17
GAGTACA229500.015.7990941
GTATAAT25600.015.4312051
AGTACAT222100.015.2582572
TACTTTT266650.015.22158214-15
GTATATA32050.015.1136621
TGGGGGG104950.015.04565056
GTACCTG70050.014.836951
GTATATG26500.014.0197961
Produced by FastQC (version 0.11.5)