FastQCFastQC Report
Mon 6 Jun 2016
ERR523120_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523120_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8910472
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217550.24415092713382636No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183120.20551099874394982No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109960.1234053594467274No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117450.054.443151
GTATCAA212850.040.6743771
TCAACGC263850.032.0078744
CAACGCA268550.031.4459235
ATCAACG273550.030.8935433
AACGCAG275400.030.6899576
TATCAAC282700.030.1810232
GTACATG308700.030.0723421
TACATGG323100.028.3277552
ACATGGG330200.026.988333
ACGCAGA320350.026.3542087
CGCAGAG335650.025.025948
CATGGGG230750.024.7253724
GCAGAGT379350.021.9807939
ATGGGGG125100.020.3226995
GAGTACT221200.020.13525612-13
GAGTACA236200.019.9004271
AGAGTAC345100.019.77867510-11
CAGAGTA366200.019.77432810-11
AGTACAT228950.019.1568532
Produced by FastQC (version 0.11.5)