FastQCFastQC Report
Mon 6 Jun 2016
ERR523120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523120_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8910472
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186790.2096297480088597No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168180.18874421018325405No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101800.1142475954135763No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112600.053.022941
GTATCAA198900.040.1646041
TCAACGC245700.032.0177654
CAACGCA250750.031.4291635
ATCAACG256000.030.7127463
AACGCAG260050.030.3771276
TATCAAC264750.029.8983522
GTACATG299500.027.9931321
TACATGG310500.026.644242
ACGCAGA305900.025.7928757
ACATGGG318950.025.2994083
CGCAGAG323600.024.3878258
CATGGGG221250.023.725244
GCAGAGT365250.021.4781579
GAGTACA234150.020.253821
GAGTACT209350.019.41021212-13
AGTACAT229850.019.3982682
AGAGTAC330700.019.26653110-11
CAGAGTA353500.019.1208710-11
ATGGGGG121750.018.4887375
Produced by FastQC (version 0.11.5)