FastQCFastQC Report
Mon 6 Jun 2016
ERR523119_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523119_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7435931
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT586610.7888857494777721No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255970.3442339634404891No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206370.27753081624883286No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT155660.20933491717446007No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC124500.16743027873712116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121010.1627368516464179No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC118220.15898479961688725No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA93320.12549874386946303No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA77080.10365884245025943No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG296500.029.2268771
TACATGG304800.027.9200442
ACATGGG303250.027.2424953
CATGGGG181900.024.5537014
ATGGGGG110550.021.7739525
GAGTACT219050.021.68289812-13
CATGGGA113600.020.4444144
GTACTTT238400.019.79489114-15
GAGTACA221800.019.3761021
AGAGTAC348600.019.18932710-11
AGTACAT215950.018.528012
AGTACTT235100.018.44414312-13
ATCGCGT2304.7011832E-516.3398518
GTGTAGC35850.016.263511
ACTTTTT293000.016.1378916-17
CGTATAC5600.015.9529683
CATGGGT49300.015.830234
CCTATAC21650.015.4197153
GTATAGG24800.014.788521
TACTTTT287850.014.72958114-15
Produced by FastQC (version 0.11.5)