FastQCFastQC Report
Mon 6 Jun 2016
ERR523119_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523119_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7435931
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT587040.7894640227296353No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236060.3174585670577094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198700.26721603522141346No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT159160.2140417924803229No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC126600.17025440392063887No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC119940.16129789262433986No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115460.15527309223283542No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA98200.1320614728673518No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG96770.1301383780995278No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC76630.10305367276807706No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG299500.027.7614961
TACATGG307200.026.5605852
ACATGGG307450.025.9240023
CATGGGG181750.024.2279034
ATGGGGG108800.022.3310645
GAGTACT216700.020.99864412-13
GTACTTT234500.019.2917414-15
GAGTACA226800.019.1595971
AGAGTAC349700.018.9202810-11
AGTACAT218700.018.7618032
CATGGGA121450.018.5348344
AGTACTT231850.017.89297112-13
GTATAGG25700.017.7499071
GTGTAGC37600.016.384791
ACTTTTT284700.015.96241116-17
CATGGGT49200.015.2828934
TACCTGG45650.015.2380732
TGGGGGG109050.015.0831856
TACTTTT280900.014.56567114-15
GTATAGT27100.014.056371
Produced by FastQC (version 0.11.5)