FastQCFastQC Report
Mon 6 Jun 2016
ERR523118_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523118_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7390482
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278280.3765383638036058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236340.31978969707253196No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136240.1843452159141988No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG82900.039.19662
GTACCTG95750.034.4346431
ACCTGGG104250.030.2220293
GTATCAA383000.025.8505421
GGTATCA275550.024.648081
TCAACGC429250.022.6862264
CAACGCA432150.022.5647895
GTACATG339000.022.462781
ATCAACG436650.022.324963
AACGCAG441050.022.1622776
TATCAAC445450.022.0528342
CATGGGG171100.021.5730744
GAGTACT233550.021.43750212-13
TACATGG352850.021.309992
ACATGGG353800.020.6945343
ACGCAGA486950.020.0342467
CGCAGAG498450.019.5713628
GTACTTT260500.019.1298214-15
ATGGGGG92500.018.196945
CCTGGGG96350.018.1544744
Produced by FastQC (version 0.11.5)