FastQCFastQC Report
Mon 6 Jun 2016
ERR523118_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523118_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7390482
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242020.32747525804135647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225040.30449976063807477No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131510.17794509207924464No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG80750.039.6349032
GTACCTG97000.033.5413861
ACCTGGG99600.031.7062573
GGTATCA280800.025.3331071
GTATCAA375750.023.5111791
GTACATG345400.021.5887491
TACATGG353600.020.7341182
CATGGGG171900.020.4758034
TCAACGC426700.020.286264
CAACGCA429850.020.1265325
GAGTACT231700.020.10488912-13
ACATGGG354650.019.9951343
ATCAACG433700.019.9696733
AACGCAG439300.019.7581656
TATCAAC443600.019.6634462
CCTGGGG94500.018.8967384
ACGCAGA485650.017.853117
GTACTTT260550.017.82255414-15
CGCAGAG497300.017.42018
AGTACTT253800.017.4096612-13
Produced by FastQC (version 0.11.5)