FastQCFastQC Report
Mon 6 Jun 2016
ERR523117_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523117_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3672094
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130350.35497457309099384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105060.28610378710348916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60390.16445657436873892No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT54730.1490430255870356No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG55200.047.8810352
ACCTGGG62350.041.7108463
GTACCTG64200.041.389631
TATAACG3200.039.6807672
ATAACGC4600.030.6707063
CCTGGGG56000.029.5607174
TAACGCA4200.029.112834
GTACATG139400.027.4624791
TACATGG142400.026.0905572
GTATCAA192400.025.1284751
ACATGGG144950.024.8525453
CATGGGG90200.023.462074
GGTATCA140600.022.9130731
ATGGGGG59150.022.7380855
TACCGGG4500.021.9468942
TCAACGC217100.021.9003814
CAACGCA218150.021.7509655
ATCAACG219200.021.6691153
GTACCGG5700.021.4524361
GTATAAC9900.021.3774281
Produced by FastQC (version 0.11.5)