FastQCFastQC Report
Mon 6 Jun 2016
ERR523117_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523117_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3672094
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123870.3373279660052275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104520.28463323651300865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59010.16069850063751093No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT56780.15462567134719318No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG58350.051.7975122
ACCTGGG66100.044.7270433
GTACCTG68100.044.6190151
TATAACG3200.042.597762
CCTGGGG57200.033.1154024
ATAACGC5150.031.9434493
TAACGCA4600.029.6320154
TATCACG2409.094947E-1225.4607322
GGTATCA144350.024.0803181
GTACATG139550.023.8637121
CTGGGGG34800.023.6363185
TACATGG141550.023.2116532
GTATCAA189650.022.6935711
TGGGGGG53350.022.5516996
TACCGGG5650.022.4622922
CATGGGG87400.022.3181234
ACATGGG143850.022.3167953
GTACCGG6600.020.6675321
TACCGTA2551.3491444E-820.2736597
ATCAACG215750.019.7813573
Produced by FastQC (version 0.11.5)