FastQCFastQC Report
Mon 6 Jun 2016
ERR523116_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523116_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9032682
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT635730.7038108947043635No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT305500.33821626843500086No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243420.2694880656708606No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT187090.20712563555320557No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC147160.16291949611422166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137010.15168252352955633No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC128760.14254902364546876No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA110370.12218962208566625No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG103080.11411892946081796No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG318800.026.2224791
TACATGG321200.025.439182
ACATGGG328400.024.2954253
CATGGGG213550.022.4990064
GAGTACT260650.020.9370412-13
AGAGTAC406550.019.41428410-11
GTACTTT282650.019.04255714-15
ATGGGGG116150.018.4576725
AGTACTT279500.018.11295312-13
GAGTACA246250.016.1530461
ACTTTTT347850.015.77018416-17
CATGGGA121300.015.42532354
AGTACAT239050.015.2419972
TACTTTT327050.015.23616614-15
GTATAGG24950.014.6988841
GTATTAC20200.014.6638991
GTGTAGC42200.014.484091
GTATAGA30000.014.4187251
TATCACG8350.014.0761042
GTATAAT41550.013.6922441
Produced by FastQC (version 0.11.5)