FastQCFastQC Report
Mon 6 Jun 2016
ERR523116_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR523116_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9032682
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT646480.7157121218260535No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279390.3093101251654824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234300.25939139670808736No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT184960.20476753194676842No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC154650.17121160691807816No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC134630.14904764719935895No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129650.14353433454205516No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA113340.12547768204393778No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG108290.11988687302398114No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA96090.10638036410448193No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG311050.025.779691
TACATGG319150.024.4180532
ACATGGG323950.023.2398743
GAGTACT262900.021.1649312-13
CATGGGG210300.021.117584
AGAGTAC412850.019.70094110-11
GTACTTT281350.019.5309714-15
AGTACTT280400.018.46909712-13
ATGGGGG112150.017.557555
TATACCG7200.016.9703225
GAGTACA250400.016.9416521
TACCTGG64800.016.2477912
TATAACG8400.016.2270682
ACTTTTT346450.015.98695916-17
TACTTTT326050.015.64954914-15
GTATAGG24700.015.6135041
AGTACAT245500.015.4122392
TAACGCA8350.015.1959874
CATGGGA117500.015.0783944
GTACCTG75200.014.4469991
Produced by FastQC (version 0.11.5)